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Electrochemistry and electrocatalytic properties of hemoglobin in layer-by-layer films of SiO2 with vapor-surface sol-gel deposition.
Anal Chem. 2007 May 15; 79(10):3581-8.AC

Abstract

In this paper, layer-by-layer {Hb/SiO2}n films assembled by alternate adsorption of positively charged hemoglobin (Hb) and vapor-surface sol-gel deposition of silica at 50 degrees C onto a glassy carbon electrode were reported. The result films were characterized with cyclic voltametery, electrochemical impedance spectroscopy, UV-vis spectroscopy, and SEM, and the direct electrochemical and electrocatalytic properties of Hb in these layer-by-layer films were investigated. A pair of well-defined quasi-reversible cyclic voltammetric peaks were observed, and the formal potential of the heme FeIII/FeII redox couple was found to be -0.330 V(vs SCE). The electron-transfer behavior of Hb in {Hb/SiO2}n films was dependent on the vapor temperature, the number of layers, and the pH of the Hb solution, based on which a set of optimized conditions for film fabrication was inferred. The hemoglobin in{Hb/SiO2}n films displayed good electrocatalytic activity to the reduction of hydrogen peroxide, and H2O2 had linear current response from 1.0 x 10(-6) to 2.0 x 10(-4) M with a detection limit of 5.0 x 10(-7) M (S/N = 3). The apparent heterogeneous electron-transfer rate constant (ks) was 1.02 +/- 0.03 s(-1), and the apparent Michaeli-Menten constant (Kmapp) was 0.155 mM, indicating a potential application in the third-generation biosensor.

Authors+Show Affiliations

Department of Chemistry, East China Normal University, 3663 Zhongshan Road(N), Shanghai, 200062, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17437331

Citation

Shi, Guoyue, et al. "Electrochemistry and Electrocatalytic Properties of Hemoglobin in Layer-by-layer Films of SiO2 With Vapor-surface Sol-gel Deposition." Analytical Chemistry, vol. 79, no. 10, 2007, pp. 3581-8.
Shi G, Sun Z, Liu M, et al. Electrochemistry and electrocatalytic properties of hemoglobin in layer-by-layer films of SiO2 with vapor-surface sol-gel deposition. Anal Chem. 2007;79(10):3581-8.
Shi, G., Sun, Z., Liu, M., Zhang, L., Liu, Y., Qu, Y., & Jin, L. (2007). Electrochemistry and electrocatalytic properties of hemoglobin in layer-by-layer films of SiO2 with vapor-surface sol-gel deposition. Analytical Chemistry, 79(10), 3581-8.
Shi G, et al. Electrochemistry and Electrocatalytic Properties of Hemoglobin in Layer-by-layer Films of SiO2 With Vapor-surface Sol-gel Deposition. Anal Chem. 2007 May 15;79(10):3581-8. PubMed PMID: 17437331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemistry and electrocatalytic properties of hemoglobin in layer-by-layer films of SiO2 with vapor-surface sol-gel deposition. AU - Shi,Guoyue, AU - Sun,Zhiyu, AU - Liu,Meichuan, AU - Zhang,Li, AU - Liu,Ye, AU - Qu,Yunhe, AU - Jin,Litong, Y1 - 2007/04/17/ PY - 2007/4/18/pubmed PY - 2007/7/7/medline PY - 2007/4/18/entrez SP - 3581 EP - 8 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 10 N2 - In this paper, layer-by-layer {Hb/SiO2}n films assembled by alternate adsorption of positively charged hemoglobin (Hb) and vapor-surface sol-gel deposition of silica at 50 degrees C onto a glassy carbon electrode were reported. The result films were characterized with cyclic voltametery, electrochemical impedance spectroscopy, UV-vis spectroscopy, and SEM, and the direct electrochemical and electrocatalytic properties of Hb in these layer-by-layer films were investigated. A pair of well-defined quasi-reversible cyclic voltammetric peaks were observed, and the formal potential of the heme FeIII/FeII redox couple was found to be -0.330 V(vs SCE). The electron-transfer behavior of Hb in {Hb/SiO2}n films was dependent on the vapor temperature, the number of layers, and the pH of the Hb solution, based on which a set of optimized conditions for film fabrication was inferred. The hemoglobin in{Hb/SiO2}n films displayed good electrocatalytic activity to the reduction of hydrogen peroxide, and H2O2 had linear current response from 1.0 x 10(-6) to 2.0 x 10(-4) M with a detection limit of 5.0 x 10(-7) M (S/N = 3). The apparent heterogeneous electron-transfer rate constant (ks) was 1.02 +/- 0.03 s(-1), and the apparent Michaeli-Menten constant (Kmapp) was 0.155 mM, indicating a potential application in the third-generation biosensor. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17437331/Electrochemistry_and_electrocatalytic_properties_of_hemoglobin_in_layer_by_layer_films_of_SiO2_with_vapor_surface_sol_gel_deposition_ L2 - https://doi.org/10.1021/ac062034g DB - PRIME DP - Unbound Medicine ER -