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Renal tissue NO and intrarenal haemodynamics during experimental variations of NO content in anaesthetised rats.
J Physiol Pharmacol. 2007 Mar; 58(1):149-63.JP

Abstract

Direct renal nitric oxide (NO) measurements were infrequent and no simultaneous measurements of renal cortical and medullary NO and local perfusion. Large-surface NO electrodes were placed in renal cortex and medulla of anaesthetised rats; simultaneously, renal blood flow (RBF, index of cortical perfusion) and medullary laser-Doppler flux (MBF) were determined. NO synthase inhibitors: nonselective (L-NAME) or selective for neuronal NOS (nNOS) (S-methyl-thiocitrulline, SMTC), and NO donor (SNAP), were used to manipulate tissue NO. Baseline tissue NO was significantly higher in medulla (703+/-49 NM) than in cortex (231+/-17 nM). Minimal cortical and medullary NO current measured after maximal L-NAME dose (2.4 mg kg(-1) i.v.) was taken as tissue NO zero kevel. This dose decreased RBF and MBF significantly (-43%). SMTC, 1.2 mg kg(-1) h(-1) i.v., significantly decreased tissue NO by 105+/-32 nM in cortex and 546+/-64 nM in medulla, RBF and MBF decreased 30% and 20%, respectively. Renal artery infusion of SNAP, 0.24 mg kg(-1) min(-1) significantly increased tissue NO by 139+/-18 nM in cortex and 948+/-110 nM in medulla. Since inhibition of nNOS decreased medullary NO by 80% and MBF by 20% only, this isoform has probably minor role in the maintenance of medullary perfusion.

Authors+Show Affiliations

Laboratory of Renal and Body Fluid Physiology, M. Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17440233

Citation

Grzelec-Mojzesowicz, M, and J Sadowski. "Renal Tissue NO and Intrarenal Haemodynamics During Experimental Variations of NO Content in Anaesthetised Rats." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 58, no. 1, 2007, pp. 149-63.
Grzelec-Mojzesowicz M, Sadowski J. Renal tissue NO and intrarenal haemodynamics during experimental variations of NO content in anaesthetised rats. J Physiol Pharmacol. 2007;58(1):149-63.
Grzelec-Mojzesowicz, M., & Sadowski, J. (2007). Renal tissue NO and intrarenal haemodynamics during experimental variations of NO content in anaesthetised rats. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 58(1), 149-63.
Grzelec-Mojzesowicz M, Sadowski J. Renal Tissue NO and Intrarenal Haemodynamics During Experimental Variations of NO Content in Anaesthetised Rats. J Physiol Pharmacol. 2007;58(1):149-63. PubMed PMID: 17440233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Renal tissue NO and intrarenal haemodynamics during experimental variations of NO content in anaesthetised rats. AU - Grzelec-Mojzesowicz,M, AU - Sadowski,J, PY - 2006/07/20/received PY - 2007/02/02/accepted PY - 2007/4/19/pubmed PY - 2007/5/11/medline PY - 2007/4/19/entrez SP - 149 EP - 63 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 58 IS - 1 N2 - Direct renal nitric oxide (NO) measurements were infrequent and no simultaneous measurements of renal cortical and medullary NO and local perfusion. Large-surface NO electrodes were placed in renal cortex and medulla of anaesthetised rats; simultaneously, renal blood flow (RBF, index of cortical perfusion) and medullary laser-Doppler flux (MBF) were determined. NO synthase inhibitors: nonselective (L-NAME) or selective for neuronal NOS (nNOS) (S-methyl-thiocitrulline, SMTC), and NO donor (SNAP), were used to manipulate tissue NO. Baseline tissue NO was significantly higher in medulla (703+/-49 NM) than in cortex (231+/-17 nM). Minimal cortical and medullary NO current measured after maximal L-NAME dose (2.4 mg kg(-1) i.v.) was taken as tissue NO zero kevel. This dose decreased RBF and MBF significantly (-43%). SMTC, 1.2 mg kg(-1) h(-1) i.v., significantly decreased tissue NO by 105+/-32 nM in cortex and 546+/-64 nM in medulla, RBF and MBF decreased 30% and 20%, respectively. Renal artery infusion of SNAP, 0.24 mg kg(-1) min(-1) significantly increased tissue NO by 139+/-18 nM in cortex and 948+/-110 nM in medulla. Since inhibition of nNOS decreased medullary NO by 80% and MBF by 20% only, this isoform has probably minor role in the maintenance of medullary perfusion. SN - 0867-5910 UR - https://www.unboundmedicine.com/medline/citation/17440233/Renal_tissue_NO_and_intrarenal_haemodynamics_during_experimental_variations_of_NO_content_in_anaesthetised_rats_ L2 - http://www.jpp.krakow.pl/journal/archive/03_07/pdf/149_03_07_article.pdf DB - PRIME DP - Unbound Medicine ER -