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Bombesin stimulates enterocyte turnover following massive small bowel resection in a rat.
Pediatr Surg Int. 2007 May; 23(5):397-404.PS

Abstract

Recent evidence suggests that bombesin (BBS) is involved in modulation of growth and differentiation of normal small intestine. The purpose of the present study was to evaluate the effects of BBS on enterocyte turnover after massive small bowel resection in a rat. Male Sprague-Dawley rats were divided into three experimental groups: Sham rats underwent bowel transection and re-anastomosis, short bowel syndrome (SBS) rats underwent a 75% small bowel resection, and SBS-BBS rats underwent bowel resection and were treated with BBS given subcutaneously at a dose of 20 mug/kg, once daily, from postoperative day 3 through 14. Parameters of intestinal adaptation (bowel and mucosal weights, mucosal DNA and protein, villus height and crypt depth), enterocyte proliferation and enterocyte apoptosis were determined in jejunum and ileum on day 15 following operation. RT-PCR technique was used to determine Bax and Bcl-2 gene expression in ileal mucosa. Statistical analysis was performed using the non-parametric Kruskal-Wallis ANOVA test, with P less than 0.05 considered statistically significant. Treatment with BBS resulted in a significant increase in ileal bowel and mucosal weight, ileal mucosal DNA and protein, jejunal and ileal villus height, jejunal crypt depth, and jejunal and ileal proliferation index compared to SBS-animals. SBS rats showed a significant increase in Bax and Bcl-2 expression in ileum that was accompanied by a significant increase in cell apoptosis compared to sham animals. SBS-BBS rats demonstrated a significant decrease in Bax and Bcl-2 expression in ileum and a decrease in apoptotic index compared to SBS-animals. In conclusion, in a rat model of SBS, BBS enhances enterocyte turnover and stimulates structural intestinal adaptation. Decreased Bax expression may be responsible for the inhibitory effect of BBS on enterocyte apoptosis.

Authors+Show Affiliations

Department of Pediatric Surgery, Pediatrics and Pathology, Bnai Zion Medical Center, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, 47 Golomb St., P.O.B. 4940, Haifa 31048, Israel. igor-dr@internet-zahav.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17440764

Citation

Sukhotnik, Igor, et al. "Bombesin Stimulates Enterocyte Turnover Following Massive Small Bowel Resection in a Rat." Pediatric Surgery International, vol. 23, no. 5, 2007, pp. 397-404.
Sukhotnik I, Slijper N, Karry R, et al. Bombesin stimulates enterocyte turnover following massive small bowel resection in a rat. Pediatr Surg Int. 2007;23(5):397-404.
Sukhotnik, I., Slijper, N., Karry, R., Shaoul, R., Coran, A. G., Lurie, M., Shiloni, E., & Mogilner, J. G. (2007). Bombesin stimulates enterocyte turnover following massive small bowel resection in a rat. Pediatric Surgery International, 23(5), 397-404.
Sukhotnik I, et al. Bombesin Stimulates Enterocyte Turnover Following Massive Small Bowel Resection in a Rat. Pediatr Surg Int. 2007;23(5):397-404. PubMed PMID: 17440764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bombesin stimulates enterocyte turnover following massive small bowel resection in a rat. AU - Sukhotnik,Igor, AU - Slijper,Nadav, AU - Karry,Rachel, AU - Shaoul,Ron, AU - Coran,Arnold G, AU - Lurie,Michael, AU - Shiloni,Eitan, AU - Mogilner,Jorge G, PY - 2007/4/19/pubmed PY - 2007/7/28/medline PY - 2007/4/19/entrez SP - 397 EP - 404 JF - Pediatric surgery international JO - Pediatr Surg Int VL - 23 IS - 5 N2 - Recent evidence suggests that bombesin (BBS) is involved in modulation of growth and differentiation of normal small intestine. The purpose of the present study was to evaluate the effects of BBS on enterocyte turnover after massive small bowel resection in a rat. Male Sprague-Dawley rats were divided into three experimental groups: Sham rats underwent bowel transection and re-anastomosis, short bowel syndrome (SBS) rats underwent a 75% small bowel resection, and SBS-BBS rats underwent bowel resection and were treated with BBS given subcutaneously at a dose of 20 mug/kg, once daily, from postoperative day 3 through 14. Parameters of intestinal adaptation (bowel and mucosal weights, mucosal DNA and protein, villus height and crypt depth), enterocyte proliferation and enterocyte apoptosis were determined in jejunum and ileum on day 15 following operation. RT-PCR technique was used to determine Bax and Bcl-2 gene expression in ileal mucosa. Statistical analysis was performed using the non-parametric Kruskal-Wallis ANOVA test, with P less than 0.05 considered statistically significant. Treatment with BBS resulted in a significant increase in ileal bowel and mucosal weight, ileal mucosal DNA and protein, jejunal and ileal villus height, jejunal crypt depth, and jejunal and ileal proliferation index compared to SBS-animals. SBS rats showed a significant increase in Bax and Bcl-2 expression in ileum that was accompanied by a significant increase in cell apoptosis compared to sham animals. SBS-BBS rats demonstrated a significant decrease in Bax and Bcl-2 expression in ileum and a decrease in apoptotic index compared to SBS-animals. In conclusion, in a rat model of SBS, BBS enhances enterocyte turnover and stimulates structural intestinal adaptation. Decreased Bax expression may be responsible for the inhibitory effect of BBS on enterocyte apoptosis. SN - 0179-0358 UR - https://www.unboundmedicine.com/medline/citation/17440764/Bombesin_stimulates_enterocyte_turnover_following_massive_small_bowel_resection_in_a_rat_ DB - PRIME DP - Unbound Medicine ER -