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[The effect of glucocorticoid on IL-8 expressing in lung tissue of rat with chronic obstructive pulmonary disease].
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Mar; 38(2):287-90.SD

Abstract

OBJECTIVE

To explore the role of IL-8 in the pathogenesis of chronic obstructive pulmonary disease (COPD) and the effect of glucocorticoid on COPD.

METHODS

24 health males of Wistar rats were randomly divided into three groups (group A: normal control group, group B: COPD model group, group C: group pretreated with prednisone), of which each got 8 rats for this study project. Rat COPD model was established by exposing rat to cigarette smoke daily for 120 days. The prednisone was, via stomachal injection, given to the rats of group C with a dose of 5 mg/kg on every other day just before rats exposed to cigarette smoke. After COPD model was set up, the bronchoalveolar lavage (BAL) was performed. The total cells and neutrophils in bronchoalveolar lavage fluid (BALF) were counted for examination, and the levels of IL-8 and TNF-alpha in supernatants of BALF and serum were detected by ELISA. The lung tissue section stained by HE was observed in order to study the alternation of morphology, and also measured in terms of mean lining interval (MLI), mean alveolus number (MAN) and percentage of alveolar area in total area (PAA).

RESULTS

MLI and PAA in group B were higher than those in group A (P < 0.01), and decreased after pretreatment with prednisone (P < 0.01), in which however MAN was just on the contrary. The statistic analysis showed that levels of IL-8 [(114.5 +/- 15.7) pg/mL vs (259.4 +/- 20.1) pg/mL, respectively, P < 0.01], TNF-alpha [(80.5 +/- 9.5) pg/mL vs (145.9 +/- 17.3) pg/ mL, P < 0.01], total cell counts [(1.64 +/- 0.12) x 10(8)/L vs (5.76 +/- 0.29) x 10(8)/L, P < 0.01], neutrophil counts [(0.099 +/- 0.065) x 10(8)/L vs (1.26 +/- 0.25) x 10(8)/L P < 0.01], neutrophil proportion [(5.9 +/- 3. 6)% vs (21.8 +/- 3.7)%, P < 0.05] in BALF of group B were higher than those of group A. After pretreatment with prednisone, the above measured values were significant reduction (P < 0.01 or 0.05). The level of IL-8 in serum of group B was higher than that of group A [(45.2 +/- 13.5) pg/mL vs (85.7 +/- 7.0) pg/mL, P < 0.01], but which, after pretreatment, there was reduction but no significance (P > 0.05). The positive correlation was demonstrated among the levels of IL-8, TNF-alpha and counted neutrophils in BALF of group B, but not between the level of IL-8 and TNF-alpha in serum.

CONCLUSION

There is a close correlation between IL-8 in BALF and airway inflammation with COPD. The IL-8 cooperating with TNF-alpha may be responsible for the persistence and amplification of airway inflammation with COPD. By modulating the expression of IL-8 gene, inhibiting granulocyte chemotaxis and degranulation, the prednisone may relieve airway inflammation, and relieve the lesion of airway resulting from inflammatory cytokines and cells, hence postpone the progress of COPD.

Authors+Show Affiliations

Department of Respiratory Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17441351

Citation

Zhang, Yong, et al. "[The Effect of Glucocorticoid On IL-8 Expressing in Lung Tissue of Rat With Chronic Obstructive Pulmonary Disease]." Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, vol. 38, no. 2, 2007, pp. 287-90.
Zhang Y, Yang SR, Cheng DY. [The effect of glucocorticoid on IL-8 expressing in lung tissue of rat with chronic obstructive pulmonary disease]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2007;38(2):287-90.
Zhang, Y., Yang, S. R., & Cheng, D. Y. (2007). [The effect of glucocorticoid on IL-8 expressing in lung tissue of rat with chronic obstructive pulmonary disease]. Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, 38(2), 287-90.
Zhang Y, Yang SR, Cheng DY. [The Effect of Glucocorticoid On IL-8 Expressing in Lung Tissue of Rat With Chronic Obstructive Pulmonary Disease]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2007;38(2):287-90. PubMed PMID: 17441351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The effect of glucocorticoid on IL-8 expressing in lung tissue of rat with chronic obstructive pulmonary disease]. AU - Zhang,Yong, AU - Yang,Shao-Rong, AU - Cheng,De-Yun, PY - 2007/4/20/pubmed PY - 2010/6/3/medline PY - 2007/4/20/entrez SP - 287 EP - 90 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 38 IS - 2 N2 - OBJECTIVE: To explore the role of IL-8 in the pathogenesis of chronic obstructive pulmonary disease (COPD) and the effect of glucocorticoid on COPD. METHODS: 24 health males of Wistar rats were randomly divided into three groups (group A: normal control group, group B: COPD model group, group C: group pretreated with prednisone), of which each got 8 rats for this study project. Rat COPD model was established by exposing rat to cigarette smoke daily for 120 days. The prednisone was, via stomachal injection, given to the rats of group C with a dose of 5 mg/kg on every other day just before rats exposed to cigarette smoke. After COPD model was set up, the bronchoalveolar lavage (BAL) was performed. The total cells and neutrophils in bronchoalveolar lavage fluid (BALF) were counted for examination, and the levels of IL-8 and TNF-alpha in supernatants of BALF and serum were detected by ELISA. The lung tissue section stained by HE was observed in order to study the alternation of morphology, and also measured in terms of mean lining interval (MLI), mean alveolus number (MAN) and percentage of alveolar area in total area (PAA). RESULTS: MLI and PAA in group B were higher than those in group A (P < 0.01), and decreased after pretreatment with prednisone (P < 0.01), in which however MAN was just on the contrary. The statistic analysis showed that levels of IL-8 [(114.5 +/- 15.7) pg/mL vs (259.4 +/- 20.1) pg/mL, respectively, P < 0.01], TNF-alpha [(80.5 +/- 9.5) pg/mL vs (145.9 +/- 17.3) pg/ mL, P < 0.01], total cell counts [(1.64 +/- 0.12) x 10(8)/L vs (5.76 +/- 0.29) x 10(8)/L, P < 0.01], neutrophil counts [(0.099 +/- 0.065) x 10(8)/L vs (1.26 +/- 0.25) x 10(8)/L P < 0.01], neutrophil proportion [(5.9 +/- 3. 6)% vs (21.8 +/- 3.7)%, P < 0.05] in BALF of group B were higher than those of group A. After pretreatment with prednisone, the above measured values were significant reduction (P < 0.01 or 0.05). The level of IL-8 in serum of group B was higher than that of group A [(45.2 +/- 13.5) pg/mL vs (85.7 +/- 7.0) pg/mL, P < 0.01], but which, after pretreatment, there was reduction but no significance (P > 0.05). The positive correlation was demonstrated among the levels of IL-8, TNF-alpha and counted neutrophils in BALF of group B, but not between the level of IL-8 and TNF-alpha in serum. CONCLUSION: There is a close correlation between IL-8 in BALF and airway inflammation with COPD. The IL-8 cooperating with TNF-alpha may be responsible for the persistence and amplification of airway inflammation with COPD. By modulating the expression of IL-8 gene, inhibiting granulocyte chemotaxis and degranulation, the prednisone may relieve airway inflammation, and relieve the lesion of airway resulting from inflammatory cytokines and cells, hence postpone the progress of COPD. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/17441351/[The_effect_of_glucocorticoid_on_IL_8_expressing_in_lung_tissue_of_rat_with_chronic_obstructive_pulmonary_disease]_ L2 - https://medlineplus.gov/copd.html DB - PRIME DP - Unbound Medicine ER -