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Role of spinal voltage-dependent calcium channel alpha 2 delta-1 subunit in the expression of a neuropathic pain-like state in mice.
Life Sci. 2007 May 08; 80(22):2015-24.LS

Abstract

The present study was undertaken to investigate the role of spinal voltage-dependent calcium channel alpha(2)delta-1 subunit in the expression of a neuropathic pain-like state induced by partial sciatic nerve ligation in mice. In cultured spinal neurons, gabapentin (GBP), which displays the inhibitory effect of alpha(2)delta-1 subunit, suppressed the extracellular Ca(2+) influx induced by KCl, whereas it failed to inhibit the intracellular Ca(2+) release induced by inositol-1,4,5-triphosphate. Seven days after sciatic nerve ligation, the protein level of alpha(2)delta-1 subunit in the ipsilateral spinal cord was clearly increased compared to that observed in sham-operated mice. In addition, the mRNA level of alpha(2)delta-1 subunit was significantly increased in the dorsal root ganglion, but not in the spinal cord, of nerve-ligated mice. Under these conditions, a marked decrease in the latency of paw-withdrawal against a thermal stimulation and tactile stimulation, induced by sciatic nerve ligation was abolished by repeated intrathecal (i.t.) treatment with GBP. Additionally, the persistent reduction in the nociceptive threshold by i.t. treatment with GBP at the early stage of the neuropathic pain-like state was maintained for 7 days even after GBP withdrawal. It is of interest to note that a single i.t. post-injection of GBP showed a marked and transient inhibitory effect on the developed neuropathic pain-like state, whereas repeated i.t. post-treatment with GBP produced a persistent inhibitory effect during the treatment. In conclusion, we propose here that the neuropathic pain-like state with sciatic nerve ligation is associated with the increased level of the alpha(2)delta-1 subunit of Ca(2+) channels at the sensory nerve terminal in the spinal dorsal horn of mice. Furthermore, the present data provide evidence that the neuropathic pain may be effectively controlled by repeated treatment with GBP at the early stage.

Authors+Show Affiliations

Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan. narita@hoshi.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17442347

Citation

Narita, Minoru, et al. "Role of Spinal Voltage-dependent Calcium Channel Alpha 2 Delta-1 Subunit in the Expression of a Neuropathic Pain-like State in Mice." Life Sciences, vol. 80, no. 22, 2007, pp. 2015-24.
Narita M, Nakajima M, Miyoshi K, et al. Role of spinal voltage-dependent calcium channel alpha 2 delta-1 subunit in the expression of a neuropathic pain-like state in mice. Life Sci. 2007;80(22):2015-24.
Narita, M., Nakajima, M., Miyoshi, K., Narita, M., Nagumo, Y., Miyatake, M., Yajima, Y., Yanagida, K., Yamazaki, M., & Suzuki, T. (2007). Role of spinal voltage-dependent calcium channel alpha 2 delta-1 subunit in the expression of a neuropathic pain-like state in mice. Life Sciences, 80(22), 2015-24.
Narita M, et al. Role of Spinal Voltage-dependent Calcium Channel Alpha 2 Delta-1 Subunit in the Expression of a Neuropathic Pain-like State in Mice. Life Sci. 2007 May 8;80(22):2015-24. PubMed PMID: 17442347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of spinal voltage-dependent calcium channel alpha 2 delta-1 subunit in the expression of a neuropathic pain-like state in mice. AU - Narita,Minoru, AU - Nakajima,Mayumi, AU - Miyoshi,Kan, AU - Narita,Michiko, AU - Nagumo,Yasuyuki, AU - Miyatake,Mayumi, AU - Yajima,Yoshinori, AU - Yanagida,Kiyomi, AU - Yamazaki,Mitsuaki, AU - Suzuki,Tsutomu, Y1 - 2007/03/21/ PY - 2006/11/15/received PY - 2007/02/13/revised PY - 2007/02/28/accepted PY - 2007/4/20/pubmed PY - 2007/6/21/medline PY - 2007/4/20/entrez SP - 2015 EP - 24 JF - Life sciences JO - Life Sci VL - 80 IS - 22 N2 - The present study was undertaken to investigate the role of spinal voltage-dependent calcium channel alpha(2)delta-1 subunit in the expression of a neuropathic pain-like state induced by partial sciatic nerve ligation in mice. In cultured spinal neurons, gabapentin (GBP), which displays the inhibitory effect of alpha(2)delta-1 subunit, suppressed the extracellular Ca(2+) influx induced by KCl, whereas it failed to inhibit the intracellular Ca(2+) release induced by inositol-1,4,5-triphosphate. Seven days after sciatic nerve ligation, the protein level of alpha(2)delta-1 subunit in the ipsilateral spinal cord was clearly increased compared to that observed in sham-operated mice. In addition, the mRNA level of alpha(2)delta-1 subunit was significantly increased in the dorsal root ganglion, but not in the spinal cord, of nerve-ligated mice. Under these conditions, a marked decrease in the latency of paw-withdrawal against a thermal stimulation and tactile stimulation, induced by sciatic nerve ligation was abolished by repeated intrathecal (i.t.) treatment with GBP. Additionally, the persistent reduction in the nociceptive threshold by i.t. treatment with GBP at the early stage of the neuropathic pain-like state was maintained for 7 days even after GBP withdrawal. It is of interest to note that a single i.t. post-injection of GBP showed a marked and transient inhibitory effect on the developed neuropathic pain-like state, whereas repeated i.t. post-treatment with GBP produced a persistent inhibitory effect during the treatment. In conclusion, we propose here that the neuropathic pain-like state with sciatic nerve ligation is associated with the increased level of the alpha(2)delta-1 subunit of Ca(2+) channels at the sensory nerve terminal in the spinal dorsal horn of mice. Furthermore, the present data provide evidence that the neuropathic pain may be effectively controlled by repeated treatment with GBP at the early stage. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/17442347/Role_of_spinal_voltage_dependent_calcium_channel_alpha_2_delta_1_subunit_in_the_expression_of_a_neuropathic_pain_like_state_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(07)00241-X DB - PRIME DP - Unbound Medicine ER -