TY - JOUR T1 - Preparative isolation and purification of triterpene saponins from Clematis mandshurica by high-speed counter-current chromatography coupled with evaporative light scattering detection. AU - Shi,Shepo, AU - Jiang,Dan, AU - Zhao,Mingbo, AU - Tu,Pengfei, Y1 - 2007/03/19/ PY - 2006/12/12/received PY - 2007/01/19/revised PY - 2007/02/18/accepted PY - 2007/4/20/pubmed PY - 2007/8/2/medline PY - 2007/4/20/entrez SP - 679 EP - 83 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 852 IS - 1-2 N2 - Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to the isolation and purification of four triterpene saponins from Clematis mandshurica by one-step separation. The solvent system was composed of ethyl acetate-n-butanol-ethanol-0.05% TFA (5:10:2:20, v/v). The purities of all the saponins obtained were better than 97%. The structures of the compounds were elucidated by the means of spectroscopic methods. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17442637/Preparative_isolation_and_purification_of_triterpene_saponins_from_Clematis_mandshurica_by_high_speed_counter_current_chromatography_coupled_with_evaporative_light_scattering_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00188-2 DB - PRIME DP - Unbound Medicine ER -