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GABAergic phenotype of periglomerular cells in the rodent olfactory bulb.
J Comp Neurol 2007; 502(6):990-1002JC

Abstract

Periglomerular (PG) cells in the rodent olfactory bulb are heterogeneous anatomically and neurochemically. Here we investigated whether major classes of PG cells use gamma-aminobutyric acid (GABA) as a neurotransmitter. In addition to three known subtypes of PG cells expressing tyrosine hydroxylase (TH), calbindin D-28k (CB), and calretinin (CR), we identified a novel PG cell population containing the GABAA receptor alpha5 subunit. Consistent with previous studies in the rat, we found that TH-positive cells were also labeled with antibodies against GABA, whereas PG cells expressing CB or the alpha5 subunit were GABA-negative. Using GAD67-GFP knockin mice, we found that all PG cell subtypes expressed GAD67-GFP. Calretinin labeled the major fraction (44%) of green fluorescent protein (GFP)-positive cells, followed by TH (16%), CB (14%), and the alpha5 subunit (13%). There was no overlap between these neuronal populations, which accounted for approximately 85% of GAD67-GFP-positive cells. We then demonstrated that PG cells labeled for TH, CB, or CR established dendrodendritic synapses expressing glutamic acid decarboxylase (GAD) or the vesicular inhibitory amino acid transporter, VGAT, irrespective of their immunoreactivity for GABA. In addition, CB-, CR-, and TH-positive dendrites were apposed to GABAA receptor clusters containing the alpha1 or alpha3 subunits, which are found in mitral and tufted cells, and the alpha2 subunit, which is expressed by PG cells. Together, these findings indicate that all major subtypes of PG cells are GABAergic. In addition, they show that PG cells provide GABAergic input to the dendrites of principal neurons and are interconnected with other GABAergic interneurons, which most likely are other PG cells.

Authors+Show Affiliations

Department of Anatomy, Pharmacology, and Forensic Medicine, and Istituto Nazionale di Neuroscienze, University of Turin, I-10126 Torino, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17444497

Citation

Panzanelli, P, et al. "GABAergic Phenotype of Periglomerular Cells in the Rodent Olfactory Bulb." The Journal of Comparative Neurology, vol. 502, no. 6, 2007, pp. 990-1002.
Panzanelli P, Fritschy JM, Yanagawa Y, et al. GABAergic phenotype of periglomerular cells in the rodent olfactory bulb. J Comp Neurol. 2007;502(6):990-1002.
Panzanelli, P., Fritschy, J. M., Yanagawa, Y., Obata, K., & Sassoè-Pognetto, M. (2007). GABAergic phenotype of periglomerular cells in the rodent olfactory bulb. The Journal of Comparative Neurology, 502(6), pp. 990-1002.
Panzanelli P, et al. GABAergic Phenotype of Periglomerular Cells in the Rodent Olfactory Bulb. J Comp Neurol. 2007 Jun 20;502(6):990-1002. PubMed PMID: 17444497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GABAergic phenotype of periglomerular cells in the rodent olfactory bulb. AU - Panzanelli,P, AU - Fritschy,J M, AU - Yanagawa,Y, AU - Obata,K, AU - Sassoè-Pognetto,M, PY - 2007/4/21/pubmed PY - 2007/6/27/medline PY - 2007/4/21/entrez SP - 990 EP - 1002 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 502 IS - 6 N2 - Periglomerular (PG) cells in the rodent olfactory bulb are heterogeneous anatomically and neurochemically. Here we investigated whether major classes of PG cells use gamma-aminobutyric acid (GABA) as a neurotransmitter. In addition to three known subtypes of PG cells expressing tyrosine hydroxylase (TH), calbindin D-28k (CB), and calretinin (CR), we identified a novel PG cell population containing the GABAA receptor alpha5 subunit. Consistent with previous studies in the rat, we found that TH-positive cells were also labeled with antibodies against GABA, whereas PG cells expressing CB or the alpha5 subunit were GABA-negative. Using GAD67-GFP knockin mice, we found that all PG cell subtypes expressed GAD67-GFP. Calretinin labeled the major fraction (44%) of green fluorescent protein (GFP)-positive cells, followed by TH (16%), CB (14%), and the alpha5 subunit (13%). There was no overlap between these neuronal populations, which accounted for approximately 85% of GAD67-GFP-positive cells. We then demonstrated that PG cells labeled for TH, CB, or CR established dendrodendritic synapses expressing glutamic acid decarboxylase (GAD) or the vesicular inhibitory amino acid transporter, VGAT, irrespective of their immunoreactivity for GABA. In addition, CB-, CR-, and TH-positive dendrites were apposed to GABAA receptor clusters containing the alpha1 or alpha3 subunits, which are found in mitral and tufted cells, and the alpha2 subunit, which is expressed by PG cells. Together, these findings indicate that all major subtypes of PG cells are GABAergic. In addition, they show that PG cells provide GABAergic input to the dendrites of principal neurons and are interconnected with other GABAergic interneurons, which most likely are other PG cells. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/17444497/GABAergic_phenotype_of_periglomerular_cells_in_the_rodent_olfactory_bulb_ L2 - https://doi.org/10.1002/cne.21356 DB - PRIME DP - Unbound Medicine ER -