Tags

Type your tag names separated by a space and hit enter

Epigallocatechin-3-gallate suppresses NF-kappaB activation and phosphorylation of p38 MAPK and JNK in human astrocytoma U373MG cells.
J Nutr Biochem 2007; 18(9):587-96JN

Abstract

Epigallocatechin-3-gallate (EGCG) is the major polyphenol component of green tea and is primarily responsible for the green tea effect. EGCG possesses two triphenolic groups in its structure. These groups are reported to be important with respect to anticarcinogenic and antioxidant effects. However, the anti-inflammatory effect of EGCG on Alzheimer's disease (AD) is still not fully understood. In this study, we investigated the effects of EGCG in attenuating the inflammatory response induced by interleukin (IL)-1beta+beta-amyloid (25-35) fragment (Abeta) in human astrocytoma, U373MG cells. EGCG significantly inhibited the IL-1beta+Abeta (25-35)-induced IL-6, IL-8, vascular endothelial growth factor (VEGF) and prostaglandin (PG)E(2) production at 24 h (P<.01). The maximal inhibition rate of IL-6, IL-8, VEGF and PGE(2) production by EGCG was approximately 54.40%, 56.01%, 69.06% and 47.03%, respectively. EGCG also attenuated the expression of cyclooxygenase-2 and activation of nuclear factor-kappaB induced by IL-1beta+Abeta (25-35). We demonstrated that EGCG suppresses IL-1beta+Abeta (25-35)-induced phosphorylation of the mitogen-activated protein kinase p38 and the c-Jun N-terminal kinase. In addition, EGCG induced the expression of mitogen-activated protein kinase phosphatase-1. These results provide new insight into the pharmacological actions of EGCG and its potential therapeutic application to various neurodegenerative diseases such as AD.

Authors+Show Affiliations

Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17446059

Citation

Kim, Su-Jin, et al. "Epigallocatechin-3-gallate Suppresses NF-kappaB Activation and Phosphorylation of P38 MAPK and JNK in Human Astrocytoma U373MG Cells." The Journal of Nutritional Biochemistry, vol. 18, no. 9, 2007, pp. 587-96.
Kim SJ, Jeong HJ, Lee KM, et al. Epigallocatechin-3-gallate suppresses NF-kappaB activation and phosphorylation of p38 MAPK and JNK in human astrocytoma U373MG cells. J Nutr Biochem. 2007;18(9):587-96.
Kim, S. J., Jeong, H. J., Lee, K. M., Myung, N. Y., An, N. H., Yang, W. M., ... Um, J. Y. (2007). Epigallocatechin-3-gallate suppresses NF-kappaB activation and phosphorylation of p38 MAPK and JNK in human astrocytoma U373MG cells. The Journal of Nutritional Biochemistry, 18(9), pp. 587-96.
Kim SJ, et al. Epigallocatechin-3-gallate Suppresses NF-kappaB Activation and Phosphorylation of P38 MAPK and JNK in Human Astrocytoma U373MG Cells. J Nutr Biochem. 2007;18(9):587-96. PubMed PMID: 17446059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epigallocatechin-3-gallate suppresses NF-kappaB activation and phosphorylation of p38 MAPK and JNK in human astrocytoma U373MG cells. AU - Kim,Su-Jin, AU - Jeong,Hyun-Ja, AU - Lee,Kang-Min, AU - Myung,Noh-Yil, AU - An,Nyeon-Hyoung, AU - Yang,Woong Mo, AU - Park,Seong Kyu, AU - Lee,Hye-Jung, AU - Hong,Seung-Heon, AU - Kim,Hyung-Min, AU - Um,Jae-Young, Y1 - 2007/04/18/ PY - 2006/05/01/received PY - 2006/11/10/revised PY - 2006/11/20/accepted PY - 2007/4/21/pubmed PY - 2007/12/6/medline PY - 2007/4/21/entrez SP - 587 EP - 96 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 18 IS - 9 N2 - Epigallocatechin-3-gallate (EGCG) is the major polyphenol component of green tea and is primarily responsible for the green tea effect. EGCG possesses two triphenolic groups in its structure. These groups are reported to be important with respect to anticarcinogenic and antioxidant effects. However, the anti-inflammatory effect of EGCG on Alzheimer's disease (AD) is still not fully understood. In this study, we investigated the effects of EGCG in attenuating the inflammatory response induced by interleukin (IL)-1beta+beta-amyloid (25-35) fragment (Abeta) in human astrocytoma, U373MG cells. EGCG significantly inhibited the IL-1beta+Abeta (25-35)-induced IL-6, IL-8, vascular endothelial growth factor (VEGF) and prostaglandin (PG)E(2) production at 24 h (P<.01). The maximal inhibition rate of IL-6, IL-8, VEGF and PGE(2) production by EGCG was approximately 54.40%, 56.01%, 69.06% and 47.03%, respectively. EGCG also attenuated the expression of cyclooxygenase-2 and activation of nuclear factor-kappaB induced by IL-1beta+Abeta (25-35). We demonstrated that EGCG suppresses IL-1beta+Abeta (25-35)-induced phosphorylation of the mitogen-activated protein kinase p38 and the c-Jun N-terminal kinase. In addition, EGCG induced the expression of mitogen-activated protein kinase phosphatase-1. These results provide new insight into the pharmacological actions of EGCG and its potential therapeutic application to various neurodegenerative diseases such as AD. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/17446059/Epigallocatechin_3_gallate_suppresses_NF_kappaB_activation_and_phosphorylation_of_p38_MAPK_and_JNK_in_human_astrocytoma_U373MG_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(06)00267-1 DB - PRIME DP - Unbound Medicine ER -