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An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation.
Endocrinology 2007; 148(7):3441-8E

Abstract

A single bout of exercise increases expression of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha mRNA, which may promote mitochondrial biogenesis in skeletal muscle. In brown adipose tissue, cold exposure up-regulates PGC-1alpha expression via adrenergic receptor (AR) activation. Because exercise also activates the sympathetic nervous system, we examined whether exercise-induced increase in PGC-1alpha mRNA expression in skeletal muscle was mediated via AR activation. In C57BL/6J mice, injection of the beta2-AR agonist clenbuterol, but not alpha-, beta1-, or beta3-AR agonists, increased PGC-1alpha mRNA expression more than 30-fold in skeletal muscle. The clenbuterol-induced increase in PGC-1alpha mRNA expression in mice was inhibited by pretreatment with the beta-AR antagonist propranolol. In ex vivo experiments, direct exposure of rat epitrochlearis to beta2-AR agonist, but not alpha-, beta1-, and beta3-AR agonist, led to an increase in levels of PGC-1alpha mRNA. Injection of beta2-AR agonist did not increase PGC-1alpha mRNA expression in beta1-, beta2-, and beta3-AR knockout mice (beta-less mice). PGC-1alpha mRNA in gastrocnemius was increased 3.5-fold in response to running on a treadmill for 45 min. The exercise-induced increase in PGC-1alpha mRNA was inhibited by approximately 70% by propranolol or the beta2-AR-specific inhibitor ICI 118,551. The exercise-induced increase in PGC-1alpha mRNA in beta-less mice was also 36% lower than that in wild-type mice. These data indicate that up-regulation of PGC-1alpha expression in skeletal muscle by exercise is mediated, at least in part, by beta-ARs activation. Among ARs, beta2-AR may mediate an increase in PGC-1alpha by exercise.

Authors+Show Affiliations

Nutritional Science Program, National Institute of Health and Nutrition, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. shinjim@nih.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17446185

Citation

Miura, Shinji, et al. "An Increase in Murine Skeletal Muscle Peroxisome Proliferator-activated Receptor-gamma Coactivator-1alpha (PGC-1alpha) mRNA in Response to Exercise Is Mediated By Beta-adrenergic Receptor Activation." Endocrinology, vol. 148, no. 7, 2007, pp. 3441-8.
Miura S, Kawanaka K, Kai Y, et al. An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation. Endocrinology. 2007;148(7):3441-8.
Miura, S., Kawanaka, K., Kai, Y., Tamura, M., Goto, M., Shiuchi, T., ... Ezaki, O. (2007). An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation. Endocrinology, 148(7), pp. 3441-8.
Miura S, et al. An Increase in Murine Skeletal Muscle Peroxisome Proliferator-activated Receptor-gamma Coactivator-1alpha (PGC-1alpha) mRNA in Response to Exercise Is Mediated By Beta-adrenergic Receptor Activation. Endocrinology. 2007;148(7):3441-8. PubMed PMID: 17446185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation. AU - Miura,Shinji, AU - Kawanaka,Kentaro, AU - Kai,Yuko, AU - Tamura,Mayumi, AU - Goto,Masahide, AU - Shiuchi,Tetsuya, AU - Minokoshi,Yasuhiko, AU - Ezaki,Osamu, Y1 - 2007/04/19/ PY - 2007/4/21/pubmed PY - 2007/10/3/medline PY - 2007/4/21/entrez SP - 3441 EP - 8 JF - Endocrinology JO - Endocrinology VL - 148 IS - 7 N2 - A single bout of exercise increases expression of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha mRNA, which may promote mitochondrial biogenesis in skeletal muscle. In brown adipose tissue, cold exposure up-regulates PGC-1alpha expression via adrenergic receptor (AR) activation. Because exercise also activates the sympathetic nervous system, we examined whether exercise-induced increase in PGC-1alpha mRNA expression in skeletal muscle was mediated via AR activation. In C57BL/6J mice, injection of the beta2-AR agonist clenbuterol, but not alpha-, beta1-, or beta3-AR agonists, increased PGC-1alpha mRNA expression more than 30-fold in skeletal muscle. The clenbuterol-induced increase in PGC-1alpha mRNA expression in mice was inhibited by pretreatment with the beta-AR antagonist propranolol. In ex vivo experiments, direct exposure of rat epitrochlearis to beta2-AR agonist, but not alpha-, beta1-, and beta3-AR agonist, led to an increase in levels of PGC-1alpha mRNA. Injection of beta2-AR agonist did not increase PGC-1alpha mRNA expression in beta1-, beta2-, and beta3-AR knockout mice (beta-less mice). PGC-1alpha mRNA in gastrocnemius was increased 3.5-fold in response to running on a treadmill for 45 min. The exercise-induced increase in PGC-1alpha mRNA was inhibited by approximately 70% by propranolol or the beta2-AR-specific inhibitor ICI 118,551. The exercise-induced increase in PGC-1alpha mRNA in beta-less mice was also 36% lower than that in wild-type mice. These data indicate that up-regulation of PGC-1alpha expression in skeletal muscle by exercise is mediated, at least in part, by beta-ARs activation. Among ARs, beta2-AR may mediate an increase in PGC-1alpha by exercise. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/17446185/An_increase_in_murine_skeletal_muscle_peroxisome_proliferator_activated_receptor_gamma_coactivator_1alpha__PGC_1alpha__mRNA_in_response_to_exercise_is_mediated_by_beta_adrenergic_receptor_activation_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2006-1646 DB - PRIME DP - Unbound Medicine ER -