Tags

Type your tag names separated by a space and hit enter

Prevalence of low-level hepatitis B viremia in patients with HBV surface antigen-negative hepatocellular carcinoma with and without hepatitis C virus infection in Japan: analysis by COBAS TaqMan real-time PCR.
Intervirology 2007; 50(4):241-4I

Abstract

OBJECTIVES

The effect of circulating low-level hepatitis B virus (HBV), defined as one of the states of 'occult HBV infection', on the development of hepatocellular carcinoma (HCC) in HBV surface antigen (HBsAg)-negative patients is controversial. In addition, the prevalence of occult HBV infection strongly depends on the sensitivity of the HBV detection method. We investigated the prevalence of low-level HBV in the serum of HBsAg-negative patients with HCC using a newly developed, sensitive method based on real-time polymerase chain reaction.

METHODS

Serum was examined for HBV DNA in 132 patients with HBsAg-negative HCC (95 with hepatitis C virus [HCV] infection and 37 without detectable hepatitis virus infection) with the COBAS TaqMan HBV test, of which the 95% hit rate is 35 copies/ml (6.7 IU/ml).

RESULTS

Low-level HBV DNA was detected in 2 of 95 (2.1%) patients with HCV-related HCC and 1 of 37 (2.7%) patients with non-viral HCC.

CONCLUSION

The prevalence of the detection of circulating low-level HBV was low in both HBsAg-negative HCC patients with HCV infection and those without detectable hepatitis virus, even with the use of the most sensitive method for the detection of HBV. Circulating low-level HBV does not appear to play an important role in hepatocarcinogenesis in HBsAg-negative HCC.

Authors+Show Affiliations

Department of Gastroenterology, Ogaki Municipal Hospital, Ogaki, Gifu, Japan. tkumada@he.mirai.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17446712

Citation

Toyoda, Hidenori, et al. "Prevalence of Low-level Hepatitis B Viremia in Patients With HBV Surface Antigen-negative Hepatocellular Carcinoma With and Without Hepatitis C Virus Infection in Japan: Analysis By COBAS TaqMan Real-time PCR." Intervirology, vol. 50, no. 4, 2007, pp. 241-4.
Toyoda H, Kumada T, Kiriyama S, et al. Prevalence of low-level hepatitis B viremia in patients with HBV surface antigen-negative hepatocellular carcinoma with and without hepatitis C virus infection in Japan: analysis by COBAS TaqMan real-time PCR. Intervirology. 2007;50(4):241-4.
Toyoda, H., Kumada, T., Kiriyama, S., Sone, Y., Tanikawa, M., Hisanaga, Y., & Kanamori, A. (2007). Prevalence of low-level hepatitis B viremia in patients with HBV surface antigen-negative hepatocellular carcinoma with and without hepatitis C virus infection in Japan: analysis by COBAS TaqMan real-time PCR. Intervirology, 50(4), pp. 241-4.
Toyoda H, et al. Prevalence of Low-level Hepatitis B Viremia in Patients With HBV Surface Antigen-negative Hepatocellular Carcinoma With and Without Hepatitis C Virus Infection in Japan: Analysis By COBAS TaqMan Real-time PCR. Intervirology. 2007;50(4):241-4. PubMed PMID: 17446712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of low-level hepatitis B viremia in patients with HBV surface antigen-negative hepatocellular carcinoma with and without hepatitis C virus infection in Japan: analysis by COBAS TaqMan real-time PCR. AU - Toyoda,Hidenori, AU - Kumada,Takashi, AU - Kiriyama,Seiki, AU - Sone,Yasuhiro, AU - Tanikawa,Makoto, AU - Hisanaga,Yasuhiro, AU - Kanamori,Akira, Y1 - 2007/04/20/ PY - 2006/09/05/received PY - 2007/01/22/accepted PY - 2007/4/21/pubmed PY - 2007/10/2/medline PY - 2007/4/21/entrez SP - 241 EP - 4 JF - Intervirology JO - Intervirology VL - 50 IS - 4 N2 - OBJECTIVES: The effect of circulating low-level hepatitis B virus (HBV), defined as one of the states of 'occult HBV infection', on the development of hepatocellular carcinoma (HCC) in HBV surface antigen (HBsAg)-negative patients is controversial. In addition, the prevalence of occult HBV infection strongly depends on the sensitivity of the HBV detection method. We investigated the prevalence of low-level HBV in the serum of HBsAg-negative patients with HCC using a newly developed, sensitive method based on real-time polymerase chain reaction. METHODS: Serum was examined for HBV DNA in 132 patients with HBsAg-negative HCC (95 with hepatitis C virus [HCV] infection and 37 without detectable hepatitis virus infection) with the COBAS TaqMan HBV test, of which the 95% hit rate is 35 copies/ml (6.7 IU/ml). RESULTS: Low-level HBV DNA was detected in 2 of 95 (2.1%) patients with HCV-related HCC and 1 of 37 (2.7%) patients with non-viral HCC. CONCLUSION: The prevalence of the detection of circulating low-level HBV was low in both HBsAg-negative HCC patients with HCV infection and those without detectable hepatitis virus, even with the use of the most sensitive method for the detection of HBV. Circulating low-level HBV does not appear to play an important role in hepatocarcinogenesis in HBsAg-negative HCC. SN - 1423-0100 UR - https://www.unboundmedicine.com/medline/citation/17446712/Prevalence_of_low_level_hepatitis_B_viremia_in_patients_with_HBV_surface_antigen_negative_hepatocellular_carcinoma_with_and_without_hepatitis_C_virus_infection_in_Japan:_analysis_by_COBAS_TaqMan_real_time_PCR_ L2 - https://www.karger.com?DOI=10.1159/000101911 DB - PRIME DP - Unbound Medicine ER -