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MHC antigen expression in human oral squamous carcinoma cell lines.
J Pathol. 1991 Oct; 165(2):129-36.JP

Abstract

This study quantified the constitutive and interferon-gamma (IFN-gamma) stimulated expression of MHC class I (HLA-ABC and beta 2 microglobulin) and class II antigens (HLA-DR, -DP, -DQ) on normal and malignant oral keratinocytes using radioimmunoassay and immunocytochemical techniques. Normal keratinocytes and three of four malignant cell lines (H103, H157, H314) expressed MHC class I antigens constitutively; IFN-gamma increased MHC class I expression with significant changes in normals, H157 and H314. Normal keratinocytes expressed significantly more constitutive MHC class I antigens than H103 and H157 and significantly more IFN-gamma stimulated MHC class I antigens than H103, H157 and H314. MHC class II antigens predominantly were not expressed constitutively on normals, H103 and H157 but, in H314, HLA-DR, -DP and -DQ antigens were demonstrated on 35, 11 and 5 per cent of cells, respectively, and resulted in a non-coordinated pattern of expression (HLA-DR greater than -DP = -DQ). IFN-gamma induced HLA-DR on normals, H103 and H157, whilst HLA-DP and -DQ remained undetectable. In H314, IFN-gamma enhanced HLA-DR, -DP and -DQ (significant increase of HLA-DQ) but the interrelationship between these antigens was maintained (HLA-DR greater than -DP = -DQ). Normal keratinocytes expressed significantly more IFN-gamma stimulated HLA-DR than H103 and H157 but significantly less HLA-DR than H314 under similar experimental conditions. One oral malignant cell line (H191) did not express MHC class I and MHC class II antigens either constitutively or in response to IFN-gamma. The results demonstrate aberrant patterns of MHC expression (absence, enhanced, diminished) in the different malignant oral keratinocyte cell lines.

Authors+Show Affiliations

Department of Oral Medicine, University of Bristol, U.K.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1744799

Citation

Mutlu, S, et al. "MHC Antigen Expression in Human Oral Squamous Carcinoma Cell Lines." The Journal of Pathology, vol. 165, no. 2, 1991, pp. 129-36.
Mutlu S, Matthews JB, Midda M, et al. MHC antigen expression in human oral squamous carcinoma cell lines. J Pathol. 1991;165(2):129-36.
Mutlu, S., Matthews, J. B., Midda, M., Scully, C., & Prime, S. S. (1991). MHC antigen expression in human oral squamous carcinoma cell lines. The Journal of Pathology, 165(2), 129-36.
Mutlu S, et al. MHC Antigen Expression in Human Oral Squamous Carcinoma Cell Lines. J Pathol. 1991;165(2):129-36. PubMed PMID: 1744799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MHC antigen expression in human oral squamous carcinoma cell lines. AU - Mutlu,S, AU - Matthews,J B, AU - Midda,M, AU - Scully,C, AU - Prime,S S, PY - 1991/10/1/pubmed PY - 1991/10/1/medline PY - 1991/10/1/entrez SP - 129 EP - 36 JF - The Journal of pathology JO - J Pathol VL - 165 IS - 2 N2 - This study quantified the constitutive and interferon-gamma (IFN-gamma) stimulated expression of MHC class I (HLA-ABC and beta 2 microglobulin) and class II antigens (HLA-DR, -DP, -DQ) on normal and malignant oral keratinocytes using radioimmunoassay and immunocytochemical techniques. Normal keratinocytes and three of four malignant cell lines (H103, H157, H314) expressed MHC class I antigens constitutively; IFN-gamma increased MHC class I expression with significant changes in normals, H157 and H314. Normal keratinocytes expressed significantly more constitutive MHC class I antigens than H103 and H157 and significantly more IFN-gamma stimulated MHC class I antigens than H103, H157 and H314. MHC class II antigens predominantly were not expressed constitutively on normals, H103 and H157 but, in H314, HLA-DR, -DP and -DQ antigens were demonstrated on 35, 11 and 5 per cent of cells, respectively, and resulted in a non-coordinated pattern of expression (HLA-DR greater than -DP = -DQ). IFN-gamma induced HLA-DR on normals, H103 and H157, whilst HLA-DP and -DQ remained undetectable. In H314, IFN-gamma enhanced HLA-DR, -DP and -DQ (significant increase of HLA-DQ) but the interrelationship between these antigens was maintained (HLA-DR greater than -DP = -DQ). Normal keratinocytes expressed significantly more IFN-gamma stimulated HLA-DR than H103 and H157 but significantly less HLA-DR than H314 under similar experimental conditions. One oral malignant cell line (H191) did not express MHC class I and MHC class II antigens either constitutively or in response to IFN-gamma. The results demonstrate aberrant patterns of MHC expression (absence, enhanced, diminished) in the different malignant oral keratinocyte cell lines. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/1744799/MHC_antigen_expression_in_human_oral_squamous_carcinoma_cell_lines_ L2 - https://doi.org/10.1002/path.1711650208 DB - PRIME DP - Unbound Medicine ER -