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Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices.
J Chromatogr A. 2007 Jun 22; 1154(1-2):87-96.JC

Abstract

A solvent extraction procedure of freeze-dried aliquots followed by the analysis of phenolic compounds by reversed-phase high-performance liquid chromatography (RP-HPLC) with photodiode array detection (DAD) has been developed for the analysis of polyphenolic compounds in fruit juices. This methodology is focussed on the characterization of fruit juices, mainly for quality control purposes. The effects of experimental variables, such as solvent composition and volume and time and temperature on extraction, have been studied. A unique gradient program for the separation of several phenolic classes (hydroquinones, hydroxybenzoic acids, flavan-3-oles, hydroxycinnamic acids, coumarins, flavanones, flavones, dihydrochalcones and flavonols) has been optimized, using standards of 55 commercially available phenolic compounds present in fruits, as well as representative real extracts from fruit juices. All phenolic compounds showed a high repeatability within-day (n=5) and between days (n=3) in peak area (RSD<8%) and excellent stability of their retention times. High precision was also observed in calibration slopes (RSD<8%). Detection limits ranged between 0.005 and 0.03 microg/mL for the different detected polyphenols. Complete recoveries (98-100%) were obtained for the majority of the phenolic structures of all representative phenolic families present in fruits. The method was successfully employed to measure diverse phenolic families in juices from 18 different fruits and consequently could be used for evaluate the quality of fruit juices.

Authors+Show Affiliations

Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, P.O. Box 644, E-48080 Bilbao, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17449048

Citation

Abad-García, B, et al. "Optimization and Validation of a Methodology Based On Solvent Extraction and Liquid Chromatography for the Simultaneous Determination of Several Polyphenolic Families in Fruit Juices." Journal of Chromatography. A, vol. 1154, no. 1-2, 2007, pp. 87-96.
Abad-García B, Berrueta LA, López-Márquez DM, et al. Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices. J Chromatogr A. 2007;1154(1-2):87-96.
Abad-García, B., Berrueta, L. A., López-Márquez, D. M., Crespo-Ferrer, I., Gallo, B., & Vicente, F. (2007). Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices. Journal of Chromatography. A, 1154(1-2), 87-96.
Abad-García B, et al. Optimization and Validation of a Methodology Based On Solvent Extraction and Liquid Chromatography for the Simultaneous Determination of Several Polyphenolic Families in Fruit Juices. J Chromatogr A. 2007 Jun 22;1154(1-2):87-96. PubMed PMID: 17449048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices. AU - Abad-García,B, AU - Berrueta,L A, AU - López-Márquez,D M, AU - Crespo-Ferrer,I, AU - Gallo,B, AU - Vicente,F, Y1 - 2007/03/16/ PY - 2006/11/08/received PY - 2007/02/27/revised PY - 2007/03/05/accepted PY - 2007/4/24/pubmed PY - 2007/9/7/medline PY - 2007/4/24/entrez SP - 87 EP - 96 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1154 IS - 1-2 N2 - A solvent extraction procedure of freeze-dried aliquots followed by the analysis of phenolic compounds by reversed-phase high-performance liquid chromatography (RP-HPLC) with photodiode array detection (DAD) has been developed for the analysis of polyphenolic compounds in fruit juices. This methodology is focussed on the characterization of fruit juices, mainly for quality control purposes. The effects of experimental variables, such as solvent composition and volume and time and temperature on extraction, have been studied. A unique gradient program for the separation of several phenolic classes (hydroquinones, hydroxybenzoic acids, flavan-3-oles, hydroxycinnamic acids, coumarins, flavanones, flavones, dihydrochalcones and flavonols) has been optimized, using standards of 55 commercially available phenolic compounds present in fruits, as well as representative real extracts from fruit juices. All phenolic compounds showed a high repeatability within-day (n=5) and between days (n=3) in peak area (RSD<8%) and excellent stability of their retention times. High precision was also observed in calibration slopes (RSD<8%). Detection limits ranged between 0.005 and 0.03 microg/mL for the different detected polyphenols. Complete recoveries (98-100%) were obtained for the majority of the phenolic structures of all representative phenolic families present in fruits. The method was successfully employed to measure diverse phenolic families in juices from 18 different fruits and consequently could be used for evaluate the quality of fruit juices. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17449048/Optimization_and_validation_of_a_methodology_based_on_solvent_extraction_and_liquid_chromatography_for_the_simultaneous_determination_of_several_polyphenolic_families_in_fruit_juices_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00460-8 DB - PRIME DP - Unbound Medicine ER -