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The cross channel activities of spider neurotoxin huwentoxin-I on rat dorsal root ganglion neurons.
Biochem Biophys Res Commun. 2007 Jun 08; 357(3):579-83.BB

Abstract

In this paper, we investigated the action of huwentoxin-I (HWTX-I) purified from the venom of the Chinese bird spider Ornithoctonus huwena on Ca(2+), Na(+) channels of adult rat dorsal root ganglion (DRG) neurons. The results showed that huwentoxin-I could reduce the peak currents of N-type Ca(2+) channels (IC(50) approximately 100 nM) and TTX-S Na(+) channels (IC(50) approximately 55 nM), whereas no effect was detected on TTX-R Na(+) channels. The comparative studies indicated that the selectivity of HWTX-I on Ca(2+) channels was higher that of MVIIA and approximately the same as that of GVIA. HWTX-I is the first discovered toxin with the cross channel activities from the spider O. huwena venom similar to micro O-conotoxins MrVIA and MrVIB.

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Authors+Show Affiliations

Wang M
Key Laboratory of Protein Chemistry and Developmental Biology of Education, The College of Life Science, Hunan Normal University, Changsha 410081, China.
Guan X
No affiliation info available
Liang S
No affiliation info available

MeSH

Amino Acid SequenceAnimalsCalcium Channels, N-TypeCells, CulturedDose-Response Relationship, DrugGanglia, SpinalIon Channel GatingIon ChannelsMembrane PotentialsMolecular Sequence DataNeuronsNeurotoxinsRatsRats, Sprague-DawleyReptilian ProteinsSequence Homology, Amino AcidSodium ChannelsSpider VenomsSpidersTetrodotoxin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17451655

Citation

Wang, Meichi, et al. "The Cross Channel Activities of Spider Neurotoxin huwentoxin-I On Rat Dorsal Root Ganglion Neurons." Biochemical and Biophysical Research Communications, vol. 357, no. 3, 2007, pp. 579-83.
Wang M, Guan X, Liang S. The cross channel activities of spider neurotoxin huwentoxin-I on rat dorsal root ganglion neurons. Biochem Biophys Res Commun. 2007;357(3):579-83.
Wang, M., Guan, X., & Liang, S. (2007). The cross channel activities of spider neurotoxin huwentoxin-I on rat dorsal root ganglion neurons. Biochemical and Biophysical Research Communications, 357(3), 579-83.
Wang M, Guan X, Liang S. The Cross Channel Activities of Spider Neurotoxin huwentoxin-I On Rat Dorsal Root Ganglion Neurons. Biochem Biophys Res Commun. 2007 Jun 8;357(3):579-83. PubMed PMID: 17451655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The cross channel activities of spider neurotoxin huwentoxin-I on rat dorsal root ganglion neurons. AU - Wang,Meichi, AU - Guan,Xin, AU - Liang,Songping, Y1 - 2007/04/09/ PY - 2007/02/13/received PY - 2007/02/16/accepted PY - 2007/4/25/pubmed PY - 2007/7/13/medline PY - 2007/4/25/entrez SP - 579 EP - 83 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 357 IS - 3 N2 - In this paper, we investigated the action of huwentoxin-I (HWTX-I) purified from the venom of the Chinese bird spider Ornithoctonus huwena on Ca(2+), Na(+) channels of adult rat dorsal root ganglion (DRG) neurons. The results showed that huwentoxin-I could reduce the peak currents of N-type Ca(2+) channels (IC(50) approximately 100 nM) and TTX-S Na(+) channels (IC(50) approximately 55 nM), whereas no effect was detected on TTX-R Na(+) channels. The comparative studies indicated that the selectivity of HWTX-I on Ca(2+) channels was higher that of MVIIA and approximately the same as that of GVIA. HWTX-I is the first discovered toxin with the cross channel activities from the spider O. huwena venom similar to micro O-conotoxins MrVIA and MrVIB. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/17451655/The_cross_channel_activities_of_spider_neurotoxin_huwentoxin_I_on_rat_dorsal_root_ganglion_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(07)00373-7 DB - PRIME DP - Unbound Medicine ER -