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Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity.
Phytochemistry. 2007 May; 68(10):1428-35.P

Abstract

Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed polysaccharide fractions isolated from Grateloupia indica. The crude water extract (GiWE) as well as one fraction (F3) obtained by anion exchange chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values (0.12-1.06 microg/ml) were much lower than cytotoxic concentration 50% values (>850 microg/ml). These fractions, which were effective antiviral inhibitors if added only during the adsorption period, had very low anticoagulant activity. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Chemical, chromatographic and spectroscopic methods showed that the active polysaccharide, which has an apparent molecular mass of 60 kDa and negative specific rotation [alpha]D(32) -16 degrees (c 0.2, H2O), contains alpha-(1-->4)- and alpha-(1-->3)-linked galactopyranose residues. Sulfate groups, if present, are located mostly at C-2/6 of (1-->4)- and C-4/6 of (1-->3)-linked galactopyranosyl units, and are essential for the anti herpetic activity of this polymer.

Authors+Show Affiliations

Natural Products Laboratory, Department of Chemistry, The University of Burdwan, WB 713 104, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17451760

Citation

Chattopadhyay, Kausik, et al. "Galactan Sulfate of Grateloupia Indica: Isolation, Structural Features and Antiviral Activity." Phytochemistry, vol. 68, no. 10, 2007, pp. 1428-35.
Chattopadhyay K, Mateu CG, Mandal P, et al. Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity. Phytochemistry. 2007;68(10):1428-35.
Chattopadhyay, K., Mateu, C. G., Mandal, P., Pujol, C. A., Damonte, E. B., & Ray, B. (2007). Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity. Phytochemistry, 68(10), 1428-35.
Chattopadhyay K, et al. Galactan Sulfate of Grateloupia Indica: Isolation, Structural Features and Antiviral Activity. Phytochemistry. 2007;68(10):1428-35. PubMed PMID: 17451760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity. AU - Chattopadhyay,Kausik, AU - Mateu,Cecilia G, AU - Mandal,Pinaki, AU - Pujol,Carlos A, AU - Damonte,Elsa B, AU - Ray,Bimalendu, Y1 - 2007/04/23/ PY - 2006/10/23/received PY - 2007/02/04/revised PY - 2007/02/12/accepted PY - 2007/4/25/pubmed PY - 2007/8/8/medline PY - 2007/4/25/entrez SP - 1428 EP - 35 JF - Phytochemistry JO - Phytochemistry VL - 68 IS - 10 N2 - Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed polysaccharide fractions isolated from Grateloupia indica. The crude water extract (GiWE) as well as one fraction (F3) obtained by anion exchange chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values (0.12-1.06 microg/ml) were much lower than cytotoxic concentration 50% values (>850 microg/ml). These fractions, which were effective antiviral inhibitors if added only during the adsorption period, had very low anticoagulant activity. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Chemical, chromatographic and spectroscopic methods showed that the active polysaccharide, which has an apparent molecular mass of 60 kDa and negative specific rotation [alpha]D(32) -16 degrees (c 0.2, H2O), contains alpha-(1-->4)- and alpha-(1-->3)-linked galactopyranose residues. Sulfate groups, if present, are located mostly at C-2/6 of (1-->4)- and C-4/6 of (1-->3)-linked galactopyranosyl units, and are essential for the anti herpetic activity of this polymer. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/17451760/Galactan_sulfate_of_Grateloupia_indica:_Isolation_structural_features_and_antiviral_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(07)00090-8 DB - PRIME DP - Unbound Medicine ER -