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Characterization of the supervirulent virG gene of the Agrobacterium tumefaciens plasmid pTiBo542.
Mol Gen Genet. 1991 Nov; 230(1-2):302-9.MG

Abstract

The virG gene of the Agrobacterium tumefaciens Ti plasmid pTiBo542 has previously been reported to elicit stronger vir gene expression than its counterpart in the pTiA6 plasmid, a property we call the "superactivator" phenotype. The DNA sequence of the pTiBo542 virG gene was determined and compared to that of the pTiA6 gene. The DNA sequences of these genes differ at 16 positions: two differences are in the promoter regions, 12 are in the coding regions, and two are in the 3' untranslated regions. The 3' end of the pTiA6 virG gene also contains a probable insertion sequence that is not found downstream of the pTiBo542 gene. The base pair differences, in the two coding regions result in only two amino acid differences, both in the amino-terminal halves of the proteins. Five hybrid virG genes were constructed and used to activate the expression of a virB::lacZ gene fusion. Differences in the coding regions of these genes accounted for most of the superactivator phenotype, while differences at the promoter and 3' untranslated regions also contributed. These findings suggest that the properties of these VirG proteins and their quantities are important for vir gene induction, and also suggest a long-term selective pressure for mutations contributing to differences between these two genes.

Authors+Show Affiliations

Section of Microbiology, Cornell University, Ithaca, NY 14853.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1745238

Citation

Chen, C Y., et al. "Characterization of the Supervirulent virG Gene of the Agrobacterium Tumefaciens Plasmid PTiBo542." Molecular & General Genetics : MGG, vol. 230, no. 1-2, 1991, pp. 302-9.
Chen CY, Wang L, Winans SC. Characterization of the supervirulent virG gene of the Agrobacterium tumefaciens plasmid pTiBo542. Mol Gen Genet. 1991;230(1-2):302-9.
Chen, C. Y., Wang, L., & Winans, S. C. (1991). Characterization of the supervirulent virG gene of the Agrobacterium tumefaciens plasmid pTiBo542. Molecular & General Genetics : MGG, 230(1-2), 302-9.
Chen CY, Wang L, Winans SC. Characterization of the Supervirulent virG Gene of the Agrobacterium Tumefaciens Plasmid PTiBo542. Mol Gen Genet. 1991;230(1-2):302-9. PubMed PMID: 1745238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the supervirulent virG gene of the Agrobacterium tumefaciens plasmid pTiBo542. AU - Chen,C Y, AU - Wang,L, AU - Winans,S C, PY - 1991/11/1/pubmed PY - 1991/11/1/medline PY - 1991/11/1/entrez SP - 302 EP - 9 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 230 IS - 1-2 N2 - The virG gene of the Agrobacterium tumefaciens Ti plasmid pTiBo542 has previously been reported to elicit stronger vir gene expression than its counterpart in the pTiA6 plasmid, a property we call the "superactivator" phenotype. The DNA sequence of the pTiBo542 virG gene was determined and compared to that of the pTiA6 gene. The DNA sequences of these genes differ at 16 positions: two differences are in the promoter regions, 12 are in the coding regions, and two are in the 3' untranslated regions. The 3' end of the pTiA6 virG gene also contains a probable insertion sequence that is not found downstream of the pTiBo542 gene. The base pair differences, in the two coding regions result in only two amino acid differences, both in the amino-terminal halves of the proteins. Five hybrid virG genes were constructed and used to activate the expression of a virB::lacZ gene fusion. Differences in the coding regions of these genes accounted for most of the superactivator phenotype, while differences at the promoter and 3' untranslated regions also contributed. These findings suggest that the properties of these VirG proteins and their quantities are important for vir gene induction, and also suggest a long-term selective pressure for mutations contributing to differences between these two genes. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/1745238/Characterization_of_the_supervirulent_virG_gene_of_the_Agrobacterium_tumefaciens_plasmid_pTiBo542_ L2 - https://www.lens.org/lens/search?q=citation_id:1745238 DB - PRIME DP - Unbound Medicine ER -