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Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen.
Hum Reprod 2007; 22(7):1878-84HR

Abstract

BACKGROUND

The aim of this study was to compare the frequency of defective sperm-zona pellucida (ZP) binding (DSZPB) and defective ZP-induced acrosome reaction (DZPIAR) in subfertile men (i.e. male partners of infertile couples) with normal and abnormal semen analyses.

METHODS

A total of 1030 subfertile men with normal semen analysis (n=255), oligozoospermia (count<20x10(6)/ml, n=136), severe teratozoospermia (strict normal morphology<or=5%, n=294) and mild-moderate teratozoospermia (morphology 6-14%, n=345) were studied. Unfertilized oocytes from clinical in vitro fertilization/intracytoplasmic sperm injection were used for sperm-ZP interaction tests. After 2 h incubation of motile sperm with four oocytes, sperm tightly bound to the ZP, and the AR of ZP-bound sperm (ZPIAR) were assessed. An average of <40 sperm bound/ZP and <16% ZPIAR were used for diagnosis of DSZPB or DZPIAR.

RESULTS

For the groups of men with normal semen or mild-moderate teratozoospermia, severe teratozoospermia and oligozoospermia, the frequencies of DSZPB were: 13, 21, 29 and 28%, respectively, and in those normal SZPB, DZPIAR were 27, 36, 56 and 68%, respectively. Overall DSZPB and ZPIAR were 36, 49, 68 and 77% for the four groups, respectively. The highest frequencies of defective sperm-ZP interaction were in the oligozoospermia and severe teratozoospermia groups. In the normal and teratozoospermia groups, subjects with a relatively low sperm concentration (20-60x10(6)/ml) had a significantly higher frequency of DZPIAR.

CONCLUSION

Defective sperm-ZP interaction is a major mechanism of male infertility. DZPIAR is more frequent than DSZPB in subfertile men with either normal or abnormal semen, suggesting that sequential sperm-ZP interaction tests are essential to detect these sperm defects.

Authors+Show Affiliations

Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, 132, Grattan Street, Carlton, Victoria 3053, Australia. dyl@unimelb.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17452393

Citation

Liu, De Yi, et al. "Comparison of the Frequency of Defective Sperm-zona Pellucida (ZP) Binding and the ZP-induced Acrosome Reaction Between Subfertile Men With Normal and Abnormal Semen." Human Reproduction (Oxford, England), vol. 22, no. 7, 2007, pp. 1878-84.
Liu DY, Liu ML, Garrett C, et al. Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen. Hum Reprod. 2007;22(7):1878-84.
Liu, D. Y., Liu, M. L., Garrett, C., & Baker, H. W. (2007). Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen. Human Reproduction (Oxford, England), 22(7), pp. 1878-84.
Liu DY, et al. Comparison of the Frequency of Defective Sperm-zona Pellucida (ZP) Binding and the ZP-induced Acrosome Reaction Between Subfertile Men With Normal and Abnormal Semen. Hum Reprod. 2007;22(7):1878-84. PubMed PMID: 17452393.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen. AU - Liu,De Yi, AU - Liu,Ming Li, AU - Garrett,Claire, AU - Baker,H W Gordon, Y1 - 2007/04/23/ PY - 2007/4/25/pubmed PY - 2007/9/27/medline PY - 2007/4/25/entrez SP - 1878 EP - 84 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 22 IS - 7 N2 - BACKGROUND: The aim of this study was to compare the frequency of defective sperm-zona pellucida (ZP) binding (DSZPB) and defective ZP-induced acrosome reaction (DZPIAR) in subfertile men (i.e. male partners of infertile couples) with normal and abnormal semen analyses. METHODS: A total of 1030 subfertile men with normal semen analysis (n=255), oligozoospermia (count<20x10(6)/ml, n=136), severe teratozoospermia (strict normal morphology<or=5%, n=294) and mild-moderate teratozoospermia (morphology 6-14%, n=345) were studied. Unfertilized oocytes from clinical in vitro fertilization/intracytoplasmic sperm injection were used for sperm-ZP interaction tests. After 2 h incubation of motile sperm with four oocytes, sperm tightly bound to the ZP, and the AR of ZP-bound sperm (ZPIAR) were assessed. An average of <40 sperm bound/ZP and <16% ZPIAR were used for diagnosis of DSZPB or DZPIAR. RESULTS: For the groups of men with normal semen or mild-moderate teratozoospermia, severe teratozoospermia and oligozoospermia, the frequencies of DSZPB were: 13, 21, 29 and 28%, respectively, and in those normal SZPB, DZPIAR were 27, 36, 56 and 68%, respectively. Overall DSZPB and ZPIAR were 36, 49, 68 and 77% for the four groups, respectively. The highest frequencies of defective sperm-ZP interaction were in the oligozoospermia and severe teratozoospermia groups. In the normal and teratozoospermia groups, subjects with a relatively low sperm concentration (20-60x10(6)/ml) had a significantly higher frequency of DZPIAR. CONCLUSION: Defective sperm-ZP interaction is a major mechanism of male infertility. DZPIAR is more frequent than DSZPB in subfertile men with either normal or abnormal semen, suggesting that sequential sperm-ZP interaction tests are essential to detect these sperm defects. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/17452393/Comparison_of_the_frequency_of_defective_sperm_zona_pellucida__ZP__binding_and_the_ZP_induced_acrosome_reaction_between_subfertile_men_with_normal_and_abnormal_semen_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/dem087 DB - PRIME DP - Unbound Medicine ER -