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Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum.
J Exp Bot. 2007; 58(8):1957-67.JE

Abstract

The aerial surfaces of the common or crystalline ice plant Mesembryanthemum crystallinum L., a halophytic, facultative crassulacean acid metabolism species, are covered with specialized trichome cells called epidermal bladder cells (EBCs). EBCs are thought to serve as a peripheral salinity and/or water storage organ to improve survival under high salinity or water deficit stress conditions. However, the exact contribution of EBCs to salt tolerance in the ice plant remains poorly understood. An M. crystallinum mutant lacking EBCs was isolated from plant collections mutagenized by fast neutron irradiation. Light and electron microscopy revealed that mutant plants lacked EBCs on all surfaces of leaves and stems. Dry weight gain of aerial parts of the mutant was almost half that of wild-type plants after 3 weeks of growth at 400 mM NaCl. The EBC mutant also showed reduced leaf succulence and leaf and stem water contents compared with wild-type plants. Aerial tissues of wild-type plants had approximately 1.5-fold higher Na(+) and Cl(-) content than the mutant grown under 400 mM NaCl for 2 weeks. Na(+) and Cl(-) partitioning into EBCs of wild-type plants resulted in lower concentrations of these ions in photosynthetically active leaf tissues than in leaves of the EBC-less mutant, particularly under conditions of high salt stress. Potassium, nitrate, and phosphate ion content decreased with incorporation of NaCl into tissues in both the wild type and the mutant, but the ratios of Na(+)/K(+) and Cl(-)/NO(3)(-)content were maintained only in the leaf and stem tissues of wild-type plants. The EBC mutant showed significant impairment in plant productivity under salt stress as evaluated by seed pod and seed number and average seed weight. These results clearly show that EBCs contribute to succulence by serving as a water storage reservoir and to salt tolerance by maintaining ion sequestration and homeostasis within photosynthetically active tissues of M. crystallinum.

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Authors+Show Affiliations

Agarie S
Faculty of Agriculture, Saga University, Saga, 840-8502, Japan.
Shimoda T
No affiliation info available
Shimizu Y
No affiliation info available
Baumann K
No affiliation info available
Sunagawa H
No affiliation info available
Kondo A
No affiliation info available
Ueno O
No affiliation info available
Nakahara T
No affiliation info available
Nose A
No affiliation info available
Cushman JC
No affiliation info available

MeSH

HomeostasisMesembryanthemumMutationNitratesPhenotypePhosphatesPhotosynthesisPlant LeavesPlant StemsPotassiumReproductionSeedsSodium Chloride

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17452753

Citation

Agarie, Sakae, et al. "Salt Tolerance, Salt Accumulation, and Ionic Homeostasis in an Epidermal Bladder-cell-less Mutant of the Common Ice Plant Mesembryanthemum Crystallinum." Journal of Experimental Botany, vol. 58, no. 8, 2007, pp. 1957-67.
Agarie S, Shimoda T, Shimizu Y, et al. Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum. J Exp Bot. 2007;58(8):1957-67.
Agarie, S., Shimoda, T., Shimizu, Y., Baumann, K., Sunagawa, H., Kondo, A., Ueno, O., Nakahara, T., Nose, A., & Cushman, J. C. (2007). Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum. Journal of Experimental Botany, 58(8), 1957-67.
Agarie S, et al. Salt Tolerance, Salt Accumulation, and Ionic Homeostasis in an Epidermal Bladder-cell-less Mutant of the Common Ice Plant Mesembryanthemum Crystallinum. J Exp Bot. 2007;58(8):1957-67. PubMed PMID: 17452753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum. AU - Agarie,Sakae, AU - Shimoda,Toshifumi, AU - Shimizu,Yumi, AU - Baumann,Kathleen, AU - Sunagawa,Haruki, AU - Kondo,Ayumu, AU - Ueno,Osamu, AU - Nakahara,Teruhisa, AU - Nose,Akihiro, AU - Cushman,John C, Y1 - 2007/04/23/ PY - 2007/4/25/pubmed PY - 2007/8/31/medline PY - 2007/4/25/entrez SP - 1957 EP - 67 JF - Journal of experimental botany JO - J Exp Bot VL - 58 IS - 8 N2 - The aerial surfaces of the common or crystalline ice plant Mesembryanthemum crystallinum L., a halophytic, facultative crassulacean acid metabolism species, are covered with specialized trichome cells called epidermal bladder cells (EBCs). EBCs are thought to serve as a peripheral salinity and/or water storage organ to improve survival under high salinity or water deficit stress conditions. However, the exact contribution of EBCs to salt tolerance in the ice plant remains poorly understood. An M. crystallinum mutant lacking EBCs was isolated from plant collections mutagenized by fast neutron irradiation. Light and electron microscopy revealed that mutant plants lacked EBCs on all surfaces of leaves and stems. Dry weight gain of aerial parts of the mutant was almost half that of wild-type plants after 3 weeks of growth at 400 mM NaCl. The EBC mutant also showed reduced leaf succulence and leaf and stem water contents compared with wild-type plants. Aerial tissues of wild-type plants had approximately 1.5-fold higher Na(+) and Cl(-) content than the mutant grown under 400 mM NaCl for 2 weeks. Na(+) and Cl(-) partitioning into EBCs of wild-type plants resulted in lower concentrations of these ions in photosynthetically active leaf tissues than in leaves of the EBC-less mutant, particularly under conditions of high salt stress. Potassium, nitrate, and phosphate ion content decreased with incorporation of NaCl into tissues in both the wild type and the mutant, but the ratios of Na(+)/K(+) and Cl(-)/NO(3)(-)content were maintained only in the leaf and stem tissues of wild-type plants. The EBC mutant showed significant impairment in plant productivity under salt stress as evaluated by seed pod and seed number and average seed weight. These results clearly show that EBCs contribute to succulence by serving as a water storage reservoir and to salt tolerance by maintaining ion sequestration and homeostasis within photosynthetically active tissues of M. crystallinum. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/17452753/Salt_tolerance_salt_accumulation_and_ionic_homeostasis_in_an_epidermal_bladder_cell_less_mutant_of_the_common_ice_plant_Mesembryanthemum_crystallinum_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erm057 DB - PRIME DP - Unbound Medicine ER -