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Increases in expression of 14-3-3 eta and 14-3-3 zeta transcripts during neuroprotection induced by delta9-tetrahydrocannabinol in AF5 cells.
J Neurosci Res 2007; 85(8):1724-33JN

Abstract

The molecular mechanisms involved in N-methyl-D-aspartate (NMDA)-induced cell death and Delta9-tetrahydrocannabinol (THC)-induced neuroprotection were investigated in vitro with an AF5 neural progenitor cell line model. By microarray analysis, Ywhah, CK1, Hsp60, Pdcd 4, and Pdcd 7 were identified as being strongly regulated by both NMDA toxicity and THC neuroprotection. The 14-3-3 eta (14-3-3eta; gene symbol Ywhah) and 14-3-3 zeta (14-3-3zeta; gene symbol Ywhaz) transcripts were deceased by NMDA treatment and increased by THC treatment prior to NMDA, as measured by cDNA microarray analysis and quantitative real-time RT-PCR. Other 14-3-3 isoforms were unchanged. Whereas up-regulation of 14-3-3zeta expression was observed 30 min after treatment with THC plus NMDA, down-regulation by NMDA alone was not seen until 16 hr after treatment. By Western blotting, THC increased 14-3-3 protein only in cells that were also treated with NMDA. Overexpression of 14-3-3eta or 14-3-3zeta by transient plasmid transfection increased 14-3-3 protein levels and decreased NMDA-induced cell death. These data suggest that increases in 14-3-3 proteins mediate THC-induced neuroprotection under conditions of NMDA-induced cellular stress.

Authors+Show Affiliations

Development and Plasticity Section, Cellular Neurobiology Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, MD 21224, USA. jichen@mail.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

17455326

Citation

Chen, Jia, et al. "Increases in Expression of 14-3-3 Eta and 14-3-3 Zeta Transcripts During Neuroprotection Induced By Delta9-tetrahydrocannabinol in AF5 Cells." Journal of Neuroscience Research, vol. 85, no. 8, 2007, pp. 1724-33.
Chen J, Lee CT, Errico SL, et al. Increases in expression of 14-3-3 eta and 14-3-3 zeta transcripts during neuroprotection induced by delta9-tetrahydrocannabinol in AF5 cells. J Neurosci Res. 2007;85(8):1724-33.
Chen, J., Lee, C. T., Errico, S. L., Becker, K. G., & Freed, W. J. (2007). Increases in expression of 14-3-3 eta and 14-3-3 zeta transcripts during neuroprotection induced by delta9-tetrahydrocannabinol in AF5 cells. Journal of Neuroscience Research, 85(8), pp. 1724-33.
Chen J, et al. Increases in Expression of 14-3-3 Eta and 14-3-3 Zeta Transcripts During Neuroprotection Induced By Delta9-tetrahydrocannabinol in AF5 Cells. J Neurosci Res. 2007;85(8):1724-33. PubMed PMID: 17455326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increases in expression of 14-3-3 eta and 14-3-3 zeta transcripts during neuroprotection induced by delta9-tetrahydrocannabinol in AF5 cells. AU - Chen,Jia, AU - Lee,Chun-Ting, AU - Errico,Stacie L, AU - Becker,Kevin G, AU - Freed,William J, PY - 2007/4/25/pubmed PY - 2007/11/6/medline PY - 2007/4/25/entrez SP - 1724 EP - 33 JF - Journal of neuroscience research JO - J. Neurosci. Res. VL - 85 IS - 8 N2 - The molecular mechanisms involved in N-methyl-D-aspartate (NMDA)-induced cell death and Delta9-tetrahydrocannabinol (THC)-induced neuroprotection were investigated in vitro with an AF5 neural progenitor cell line model. By microarray analysis, Ywhah, CK1, Hsp60, Pdcd 4, and Pdcd 7 were identified as being strongly regulated by both NMDA toxicity and THC neuroprotection. The 14-3-3 eta (14-3-3eta; gene symbol Ywhah) and 14-3-3 zeta (14-3-3zeta; gene symbol Ywhaz) transcripts were deceased by NMDA treatment and increased by THC treatment prior to NMDA, as measured by cDNA microarray analysis and quantitative real-time RT-PCR. Other 14-3-3 isoforms were unchanged. Whereas up-regulation of 14-3-3zeta expression was observed 30 min after treatment with THC plus NMDA, down-regulation by NMDA alone was not seen until 16 hr after treatment. By Western blotting, THC increased 14-3-3 protein only in cells that were also treated with NMDA. Overexpression of 14-3-3eta or 14-3-3zeta by transient plasmid transfection increased 14-3-3 protein levels and decreased NMDA-induced cell death. These data suggest that increases in 14-3-3 proteins mediate THC-induced neuroprotection under conditions of NMDA-induced cellular stress. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/17455326/abstract/Increases_in_expression_of_14_3_3_eta_and_14_3_3_zeta_transcripts_during_neuroprotection_induced_by_Delta_9__tetrahydrocannabinol_in_AF5_cells L2 - https://doi.org/10.1002/jnr.21304 DB - PRIME DP - Unbound Medicine ER -