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The effect of TGF-β1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells.
Cell Mol Biol Lett. 2007 Sep; 12(3):457-72.CM

Abstract

The aim of this study was to investigate whether transforming growth factor-β1 (TGF-β1) could induce alveolar epithelial-mesenchymal transition (EMT) in vitro, and whether Smad7 gene transfer could block this transition. We also aimed to elucidate the possible mechanisms of these processes. The Smad7 gene was transfected to the rat type II alveolar epithelial cell line (RLE-6TN). Expression of the EMT-associated markers was assayed by Western Blot and Real-time PCR. Morphological alterations were examined via phase-contrast microscope and fluorescence microscope, while ultrastructural changes were examined via electron microscope. TGF-β1 treatment induced a fibrotic phenotype of RLE-6TN with increased expression of fibronectin (FN), α-smooth muscle actin (α-SMA) and vimentin, and decreased expression of E-cadherin (E-cad) and cytokeratin19 (CK19). After transfecting the RLE-6TN with the Smad7 gene, the expression of the mesenchymal markers was downregulated while that of the epithelial markers was upregulated. TGF-β1 treatment for 48 h resulted in the separation of RLE-6TN from one another and a change into elongated, myofibroblast-like cells. After the RLE-6TN had been transfected with the Smad7 gene, TGF-β1 treatment had no effect on the morphology of the RLE-6TN. TGF-β1 treatment for 48 h resulted in an abundant expression of α-SMA in the RLE-6TN. If the RLE-6TN were transfected with the Smad7 gene, TGF-β1 treatment for 48 h could only induce a low level of α-SMA expression. Furthermore, TGF-β1 treatment for 12 h resulted in the degeneration and swelling of the osmiophilic multilamellar bodies, which were the markers of type II alveolar epithelial cells. TGF-β1 can induce alveolar epithelial-mesenchymal transition in vitro, which is dependent on the Smads signaling pathway to a certain extent. Overexpression of the Smad7 gene can partially block this process.

Authors+Show Affiliations

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, 200032, China, dxz_1028@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17457524

Citation

Xu, Guo-Ping, et al. "The Effect of TGF-β1 and Smad7 Gene Transfer On the Phenotypic Changes of Rat Alveolar Epithelial Cells." Cellular & Molecular Biology Letters, vol. 12, no. 3, 2007, pp. 457-72.
Xu GP, Li QQ, Cao XX, et al. The effect of TGF-β1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells. Cell Mol Biol Lett. 2007;12(3):457-72.
Xu, G. P., Li, Q. Q., Cao, X. X., Chen, Q., Zhao, Z. H., Diao, Z. Q., & Xu, Z. D. (2007). The effect of TGF-β1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells. Cellular & Molecular Biology Letters, 12(3), 457-72. https://doi.org/10.2478/s11658-007-0018-x
Xu GP, et al. The Effect of TGF-β1 and Smad7 Gene Transfer On the Phenotypic Changes of Rat Alveolar Epithelial Cells. Cell Mol Biol Lett. 2007;12(3):457-72. PubMed PMID: 17457524.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of TGF-β1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells. AU - Xu,Guo-Ping, AU - Li,Qing-Quan, AU - Cao,Xi-Xi, AU - Chen,Qi, AU - Zhao,Zhong-Hua, AU - Diao,Zi-Qiang, AU - Xu,Zu-De, Y1 - 2007/04/24/ PY - 2006/11/13/received PY - 2007/02/20/accepted PY - 2007/4/26/pubmed PY - 2013/3/30/medline PY - 2007/4/26/entrez SP - 457 EP - 72 JF - Cellular & molecular biology letters JO - Cell Mol Biol Lett VL - 12 IS - 3 N2 - The aim of this study was to investigate whether transforming growth factor-β1 (TGF-β1) could induce alveolar epithelial-mesenchymal transition (EMT) in vitro, and whether Smad7 gene transfer could block this transition. We also aimed to elucidate the possible mechanisms of these processes. The Smad7 gene was transfected to the rat type II alveolar epithelial cell line (RLE-6TN). Expression of the EMT-associated markers was assayed by Western Blot and Real-time PCR. Morphological alterations were examined via phase-contrast microscope and fluorescence microscope, while ultrastructural changes were examined via electron microscope. TGF-β1 treatment induced a fibrotic phenotype of RLE-6TN with increased expression of fibronectin (FN), α-smooth muscle actin (α-SMA) and vimentin, and decreased expression of E-cadherin (E-cad) and cytokeratin19 (CK19). After transfecting the RLE-6TN with the Smad7 gene, the expression of the mesenchymal markers was downregulated while that of the epithelial markers was upregulated. TGF-β1 treatment for 48 h resulted in the separation of RLE-6TN from one another and a change into elongated, myofibroblast-like cells. After the RLE-6TN had been transfected with the Smad7 gene, TGF-β1 treatment had no effect on the morphology of the RLE-6TN. TGF-β1 treatment for 48 h resulted in an abundant expression of α-SMA in the RLE-6TN. If the RLE-6TN were transfected with the Smad7 gene, TGF-β1 treatment for 48 h could only induce a low level of α-SMA expression. Furthermore, TGF-β1 treatment for 12 h resulted in the degeneration and swelling of the osmiophilic multilamellar bodies, which were the markers of type II alveolar epithelial cells. TGF-β1 can induce alveolar epithelial-mesenchymal transition in vitro, which is dependent on the Smads signaling pathway to a certain extent. Overexpression of the Smad7 gene can partially block this process. SN - 1689-1392 UR - https://www.unboundmedicine.com/medline/citation/17457524/The_effect_of_TGF_��1_and_Smad7_gene_transfer_on_the_phenotypic_changes_of_rat_alveolar_epithelial_cells_ L2 - https://www.degruyter.com/document/doi/10.2478/s11658-007-0018-x DB - PRIME DP - Unbound Medicine ER -