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Ongoing subclinical infection of hepatitis E virus among blood donors with an elevated alanine aminotransferase level in Japan.
J Med Virol. 2007 Jun; 79(6):734-42.JM

Abstract

Ongoing subclinical infection of hepatitis E virus (HEV) has not been fully studied. In the present study, serum samples were collected from 6700 voluntary blood donors with an elevated alanine aminotransferase (ALT) level of 61-476 IU/l at a Japanese Red Cross Blood Center, and were tested for the presence of IgG, IgM and IgA classes of antibodies to HEV (anti-HEV) by in-house ELISA and HEV RNA by nested RT-PCR. Overall, 479 blood donors (7.1%) were positive for anti-HEV IgG, including 8 donors with anti-HEV IgM and 7 donors with anti-HEV IgA. Among the nine donors with anti-HEV IgM and/or anti-HEV IgA, six had detectable HEV RNA. The presence of HEV RNA was further tested in 10-sample minipools of sera from the remaining 6691 donors, and three donors including one without anti-HEV IgG were found to be positive for HEV RNA. When stratified by ALT level, the prevalence of HEV RNA was significantly higher among the 109 donors with ALT > or = 201 IU/l than among the 6591 donors with ALT of 61-200 IU/l (2.8% vs. 0.1%, P < 0.0001). The HEV isolates obtained from the nine viremic donors segregated into genotype 3, shared a wide range of identities of 85.6-98.5% and were 87.3-93.9% similar to the Japan-indigenous HEV strain (JRA1), in the 412-nucleotide sequence of open reading frame 2. This study suggests that approximately 3% of Japanese individuals with ALT > or = 201 IU/l have ongoing subclinical infection with various HEV strains.

Authors+Show Affiliations

Japanese Red Cross Saitama Blood Center, Saitama-Ken, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17457924

Citation

Gotanda, Yuhko, et al. "Ongoing Subclinical Infection of Hepatitis E Virus Among Blood Donors With an Elevated Alanine Aminotransferase Level in Japan." Journal of Medical Virology, vol. 79, no. 6, 2007, pp. 734-42.
Gotanda Y, Iwata A, Ohnuma H, et al. Ongoing subclinical infection of hepatitis E virus among blood donors with an elevated alanine aminotransferase level in Japan. J Med Virol. 2007;79(6):734-42.
Gotanda, Y., Iwata, A., Ohnuma, H., Yoshikawa, A., Mizoguchi, H., Endo, K., Takahashi, M., & Okamoto, H. (2007). Ongoing subclinical infection of hepatitis E virus among blood donors with an elevated alanine aminotransferase level in Japan. Journal of Medical Virology, 79(6), 734-42.
Gotanda Y, et al. Ongoing Subclinical Infection of Hepatitis E Virus Among Blood Donors With an Elevated Alanine Aminotransferase Level in Japan. J Med Virol. 2007;79(6):734-42. PubMed PMID: 17457924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ongoing subclinical infection of hepatitis E virus among blood donors with an elevated alanine aminotransferase level in Japan. AU - Gotanda,Yuhko, AU - Iwata,Akiko, AU - Ohnuma,Hitoshi, AU - Yoshikawa,Akira, AU - Mizoguchi,Hideaki, AU - Endo,Kazunori, AU - Takahashi,Masaharu, AU - Okamoto,Hiroaki, PY - 2007/4/26/pubmed PY - 2007/7/31/medline PY - 2007/4/26/entrez SP - 734 EP - 42 JF - Journal of medical virology JO - J Med Virol VL - 79 IS - 6 N2 - Ongoing subclinical infection of hepatitis E virus (HEV) has not been fully studied. In the present study, serum samples were collected from 6700 voluntary blood donors with an elevated alanine aminotransferase (ALT) level of 61-476 IU/l at a Japanese Red Cross Blood Center, and were tested for the presence of IgG, IgM and IgA classes of antibodies to HEV (anti-HEV) by in-house ELISA and HEV RNA by nested RT-PCR. Overall, 479 blood donors (7.1%) were positive for anti-HEV IgG, including 8 donors with anti-HEV IgM and 7 donors with anti-HEV IgA. Among the nine donors with anti-HEV IgM and/or anti-HEV IgA, six had detectable HEV RNA. The presence of HEV RNA was further tested in 10-sample minipools of sera from the remaining 6691 donors, and three donors including one without anti-HEV IgG were found to be positive for HEV RNA. When stratified by ALT level, the prevalence of HEV RNA was significantly higher among the 109 donors with ALT > or = 201 IU/l than among the 6591 donors with ALT of 61-200 IU/l (2.8% vs. 0.1%, P < 0.0001). The HEV isolates obtained from the nine viremic donors segregated into genotype 3, shared a wide range of identities of 85.6-98.5% and were 87.3-93.9% similar to the Japan-indigenous HEV strain (JRA1), in the 412-nucleotide sequence of open reading frame 2. This study suggests that approximately 3% of Japanese individuals with ALT > or = 201 IU/l have ongoing subclinical infection with various HEV strains. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/17457924/Ongoing_subclinical_infection_of_hepatitis_E_virus_among_blood_donors_with_an_elevated_alanine_aminotransferase_level_in_Japan_ L2 - https://doi.org/10.1002/jmv.20834 DB - PRIME DP - Unbound Medicine ER -