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AM404 decreases Fos-immunoreactivity in the spinal cord in a model of inflammatory pain.
Brain Res 2007; 1152:87-94BR

Abstract

Cannabinoids, such as anandamide, are involved in pain transmission. We evaluated the effects of AM404 (N-(4-hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide), an anandamide reuptake inhibitor, monitoring the expression of c-fos, a marker of activated neurons and the pain-related behaviours using formalin test. The study was carried out in an experimental model of inflammatory pain made by a single injection of formalin in rat hind paws. Formalin test showed that the antinociceptive effect of AM404 was evident in phase I. We found that Fos-positive neurons in dorsal superficial and deep laminae of the lumbar spinal cord increased in formalin-injected animals and that AM404 significantly reduced Fos induction. Co-administration of cannabinoid CB(1) receptor antagonist (AM251), cannabinoid CB(2) receptor antagonist (AM630) and transient receptor potential vanilloid type 1 (TRPV-1) antagonist (capsazepine), attenuate the inhibitory effect of AM404 and this effect was higher using cannabinoid CB(2) and vanilloid TRPV-1 receptor antagonists. These results suggest that AM404 could be a useful drug to reduce inflammatory pain in our experimental model and that cannabinoid CB(2) receptor and vanilloid TRPV-1 receptor, and to a lesser extent, the cannabinoid CB(1) receptor are involved.

Authors+Show Affiliations

Department of Biomedical Sciences and Biotechnologies, Division of Human Anatomy, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17459353

Citation

Borsani, Elisa, et al. "AM404 Decreases Fos-immunoreactivity in the Spinal Cord in a Model of Inflammatory Pain." Brain Research, vol. 1152, 2007, pp. 87-94.
Borsani E, Labanca M, Bianchi R, et al. AM404 decreases Fos-immunoreactivity in the spinal cord in a model of inflammatory pain. Brain Res. 2007;1152:87-94.
Borsani, E., Labanca, M., Bianchi, R., & Rodella, L. F. (2007). AM404 decreases Fos-immunoreactivity in the spinal cord in a model of inflammatory pain. Brain Research, 1152, pp. 87-94.
Borsani E, et al. AM404 Decreases Fos-immunoreactivity in the Spinal Cord in a Model of Inflammatory Pain. Brain Res. 2007 Jun 4;1152:87-94. PubMed PMID: 17459353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AM404 decreases Fos-immunoreactivity in the spinal cord in a model of inflammatory pain. AU - Borsani,Elisa, AU - Labanca,Mauro, AU - Bianchi,Rossella, AU - Rodella,Luigi F, Y1 - 2007/03/28/ PY - 2006/12/12/received PY - 2007/03/13/revised PY - 2007/03/13/accepted PY - 2007/4/27/pubmed PY - 2007/8/28/medline PY - 2007/4/27/entrez SP - 87 EP - 94 JF - Brain research JO - Brain Res. VL - 1152 N2 - Cannabinoids, such as anandamide, are involved in pain transmission. We evaluated the effects of AM404 (N-(4-hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide), an anandamide reuptake inhibitor, monitoring the expression of c-fos, a marker of activated neurons and the pain-related behaviours using formalin test. The study was carried out in an experimental model of inflammatory pain made by a single injection of formalin in rat hind paws. Formalin test showed that the antinociceptive effect of AM404 was evident in phase I. We found that Fos-positive neurons in dorsal superficial and deep laminae of the lumbar spinal cord increased in formalin-injected animals and that AM404 significantly reduced Fos induction. Co-administration of cannabinoid CB(1) receptor antagonist (AM251), cannabinoid CB(2) receptor antagonist (AM630) and transient receptor potential vanilloid type 1 (TRPV-1) antagonist (capsazepine), attenuate the inhibitory effect of AM404 and this effect was higher using cannabinoid CB(2) and vanilloid TRPV-1 receptor antagonists. These results suggest that AM404 could be a useful drug to reduce inflammatory pain in our experimental model and that cannabinoid CB(2) receptor and vanilloid TRPV-1 receptor, and to a lesser extent, the cannabinoid CB(1) receptor are involved. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/17459353/AM404_decreases_Fos_immunoreactivity_in_the_spinal_cord_in_a_model_of_inflammatory_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(07)00659-2 DB - PRIME DP - Unbound Medicine ER -