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Determination of isophorone in food samples by solid-phase microextraction coupled with gas chromatography-mass spectrometry.
J Chromatogr A. 2007 Jun 29; 1155(1):100-4.JC

Abstract

A simple and sensitive method for the determination of isophorone in food samples was developed by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Isophorone was separated within 10 min by GC-MS using a DB-1 capillary column and detected with selective ion monitoring mode. The HS-SPME using a polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber provided effective sample enrichment, and was carried out by fiber exposition at 60 degrees C for 45 min. The extracted isophorone was easily desorbed by fiber exposition in the injection port of a capillary GC-MS system, and carryover was not observed. Using this method, the calibration curve of isophorone was linear in the range 20-1000 pg/mL, with a correlation coefficient 0.9996 (n = 18), and the detection limit (S/N = 3) was 0.5 pg/mL. The HS-SPME/GC-MS method showed 25,000-fold higher sensitivity than the direct injection method (1 microL injection). The within-day and between-day precisions (relative standard deviations) at the concentration of 1 ng/mL isophorone were 3.9% and 6.1% (n=5), respectively. This method was successfully applied to the analysis of food samples without interference peaks. The recoveries of isophorone spiked into food sample were above 84% for a 50 or 500 pg/mL spiking concentration. The analytical results of the contents of isophorone in various food samples were presented.

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Authors+Show Affiliations

Kataoka H
School of Pharmacy, Shujitsu University, Nishigawara, Okayama 703-8516, Japan. hkataoka@shujitsu.ac.jp
Terada Y
No affiliation info available
Inoue R
No affiliation info available
Mitani K
No affiliation info available

MeSH

CyclohexanonesFood AnalysisGas Chromatography-Mass SpectrometryMolecular StructureReproducibility of ResultsSolid Phase Microextraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17459400

Citation

Kataoka, Hiroyuki, et al. "Determination of Isophorone in Food Samples By Solid-phase Microextraction Coupled With Gas Chromatography-mass Spectrometry." Journal of Chromatography. A, vol. 1155, no. 1, 2007, pp. 100-4.
Kataoka H, Terada Y, Inoue R, et al. Determination of isophorone in food samples by solid-phase microextraction coupled with gas chromatography-mass spectrometry. J Chromatogr A. 2007;1155(1):100-4.
Kataoka, H., Terada, Y., Inoue, R., & Mitani, K. (2007). Determination of isophorone in food samples by solid-phase microextraction coupled with gas chromatography-mass spectrometry. Journal of Chromatography. A, 1155(1), 100-4.
Kataoka H, et al. Determination of Isophorone in Food Samples By Solid-phase Microextraction Coupled With Gas Chromatography-mass Spectrometry. J Chromatogr A. 2007 Jun 29;1155(1):100-4. PubMed PMID: 17459400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of isophorone in food samples by solid-phase microextraction coupled with gas chromatography-mass spectrometry. AU - Kataoka,Hiroyuki, AU - Terada,Yohei, AU - Inoue,Reiko, AU - Mitani,Kurie, Y1 - 2007/04/12/ PY - 2007/01/30/received PY - 2007/03/30/revised PY - 2007/04/04/accepted PY - 2007/4/27/pubmed PY - 2007/8/19/medline PY - 2007/4/27/entrez SP - 100 EP - 4 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1155 IS - 1 N2 - A simple and sensitive method for the determination of isophorone in food samples was developed by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Isophorone was separated within 10 min by GC-MS using a DB-1 capillary column and detected with selective ion monitoring mode. The HS-SPME using a polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber provided effective sample enrichment, and was carried out by fiber exposition at 60 degrees C for 45 min. The extracted isophorone was easily desorbed by fiber exposition in the injection port of a capillary GC-MS system, and carryover was not observed. Using this method, the calibration curve of isophorone was linear in the range 20-1000 pg/mL, with a correlation coefficient 0.9996 (n = 18), and the detection limit (S/N = 3) was 0.5 pg/mL. The HS-SPME/GC-MS method showed 25,000-fold higher sensitivity than the direct injection method (1 microL injection). The within-day and between-day precisions (relative standard deviations) at the concentration of 1 ng/mL isophorone were 3.9% and 6.1% (n=5), respectively. This method was successfully applied to the analysis of food samples without interference peaks. The recoveries of isophorone spiked into food sample were above 84% for a 50 or 500 pg/mL spiking concentration. The analytical results of the contents of isophorone in various food samples were presented. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17459400/Determination_of_isophorone_in_food_samples_by_solid_phase_microextraction_coupled_with_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00662-0 DB - PRIME DP - Unbound Medicine ER -

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