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Simultaneous determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves by RP-HPLC with ultraviolet photodiode array detection.
J Sep Sci. 2007 Mar; 30(5):717-21.JS

Abstract

RP-HPLC with UV photodiode array detection (UV-DAD) was developed and validated for the simultaneous determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves. The analytes of interest were separated on a Diamonsil C18 column (250 x 4.6 mm id, 5 microm) with the mobile phase consisting of THF/ACN/methanol/ 0.05% phosphoric acid solution (pH 5.0) (18:1:1:80 v/vl/v). The flow rate was set at 1.0 mL/min and the eluent was detected at 340 nm for the four flavonoids. The method was linear over the studied range of 1.00-100 microg/mL for the four analytes of interest with the correlation coefficient for each analyte greater than 0.999. The LOD and LOQwere 0.03 and 0.10 microg/mL, 0.03 and 0.10 microg/mL, 0.05 and 0.15 pg/mL, 0.10 and 0.30 microg/mL for vitexin-2"-O-glucoside, vitexin-2"-0-rhamnoside, rutin, and hyperoside, respectively. The optimized method was successfully applied to the analysis of four important flavonoids in the extract of hawthorn leaves. The total amounts of the four flavonoids were 22.2, 62.3, 4.27, and 8.24 mg/g dry weight for vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn leaves, respectively.

Authors+Show Affiliations

Cheng S
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, You An Men, Beijing, China. firhill@sina.com
Qiu F
No affiliation info available
Huang J
No affiliation info available
He J
No affiliation info available

MeSH

ApigeninChromatography, High Pressure LiquidCrataegusFlavonesFlavonoidsHydrophobic and Hydrophilic InteractionsMicroarray AnalysisMolecular StructurePhotochemistryPlant ExtractsPlant LeavesQuercetinRutinSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17461112

Citation

Cheng, Shan, et al. "Simultaneous Determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, Rutin, and Hyperoside in the Extract of Hawthorn (Crataegus Pinnatifida Bge.) Leaves By RP-HPLC With Ultraviolet Photodiode Array Detection." Journal of Separation Science, vol. 30, no. 5, 2007, pp. 717-21.
Cheng S, Qiu F, Huang J, et al. Simultaneous determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves by RP-HPLC with ultraviolet photodiode array detection. J Sep Sci. 2007;30(5):717-21.
Cheng, S., Qiu, F., Huang, J., & He, J. (2007). Simultaneous determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves by RP-HPLC with ultraviolet photodiode array detection. Journal of Separation Science, 30(5), 717-21.
Cheng S, et al. Simultaneous Determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, Rutin, and Hyperoside in the Extract of Hawthorn (Crataegus Pinnatifida Bge.) Leaves By RP-HPLC With Ultraviolet Photodiode Array Detection. J Sep Sci. 2007;30(5):717-21. PubMed PMID: 17461112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves by RP-HPLC with ultraviolet photodiode array detection. AU - Cheng,Shan, AU - Qiu,Feng, AU - Huang,Jia, AU - He,Junqi, PY - 2007/4/28/pubmed PY - 2007/6/21/medline PY - 2007/4/28/entrez SP - 717 EP - 21 JF - Journal of separation science JO - J Sep Sci VL - 30 IS - 5 N2 - RP-HPLC with UV photodiode array detection (UV-DAD) was developed and validated for the simultaneous determination of vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves. The analytes of interest were separated on a Diamonsil C18 column (250 x 4.6 mm id, 5 microm) with the mobile phase consisting of THF/ACN/methanol/ 0.05% phosphoric acid solution (pH 5.0) (18:1:1:80 v/vl/v). The flow rate was set at 1.0 mL/min and the eluent was detected at 340 nm for the four flavonoids. The method was linear over the studied range of 1.00-100 microg/mL for the four analytes of interest with the correlation coefficient for each analyte greater than 0.999. The LOD and LOQwere 0.03 and 0.10 microg/mL, 0.03 and 0.10 microg/mL, 0.05 and 0.15 pg/mL, 0.10 and 0.30 microg/mL for vitexin-2"-O-glucoside, vitexin-2"-0-rhamnoside, rutin, and hyperoside, respectively. The optimized method was successfully applied to the analysis of four important flavonoids in the extract of hawthorn leaves. The total amounts of the four flavonoids were 22.2, 62.3, 4.27, and 8.24 mg/g dry weight for vitexin-2"-O-glucoside, vitexin-2"-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn leaves, respectively. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/17461112/Simultaneous_determination_of_vitexin_2"_O_glucoside_vitexin_2"_O_rhamnoside_rutin_and_hyperoside_in_the_extract_of_hawthorn__Crataegus_pinnatifida_Bge___leaves_by_RP_HPLC_with_ultraviolet_photodiode_array_detection_ L2 - https://doi.org/10.1002/jssc.200600353 DB - PRIME DP - Unbound Medicine ER -