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Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes.
World J Gastroenterol. 2007 Apr 07; 13(13):1953-61.WJ

Abstract

AIM

To investigate the hypothesis that the protective effects of curcumin in hepatic warm ischemia/reperfusion (I/R) injury are associated with increasing heat shock protein 70 (Hsp70) expression and antioxidant enzyme activity.

METHODS

Sixty Sprague-Dawley male rats were randomly divided into sham, I/R, C + I/R groups. The model of reduced-size liver warm ischemia and reperfusion was used. Curcumin (50 mg/kg) was administered by injection through a branch of superior mesenteric vein at 30 min before ischemia in C + I/R group. Five rats were used to investigate the survival during 1 wk after operation in each group. Blood samples and liver tissues were obtained in the remaining animals after 3, 12, and 24 h of reperfusion to assess serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver tissue NO(2)(-) + NO(3)(-), malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT), nitricoxide synthase (NOS) and myeloperoxidase (MPO) activity, Hsp70 expression and apoptosis ratio.

RESULTS

Compared with I/R group, curcumin pretreatment group showed less ischemia/reperfusion-induced injury. CAT and SOD activity and Hsp70 expression increased significantly. A higher rate of apoptosis was observed in I/R group than in C + I/R group, and a significant increase of MDA, NO(2)(-) + NO(3)(-) and MPO level in liver tissues and serum transaminase concentration was also observed in I/R group compared to C + I/R group. Curcumin also decreased the activity of inducible NO synthase (iNOS) in liver after reperfusion, but had no effect on the level of endothelial NO synthase (eNOS) after reperfusion in liver. The 7 d survival rate was significantly higher in C + I/R group than in I/R group.

CONCLUSION

Curcumin has protective effects against hepatic I/R injury. Its mechanism might be related to the overexpression of Hsp70 and antioxidant enzymes.

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Authors+Show Affiliations

Shen SQ
Department of General Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei Province, China. swsw2218@hotmail.com.
Zhang Y
No affiliation info available
Xiang JJ
No affiliation info available
Xiong CL
No affiliation info available

MeSH

Alanine TransaminaseAnimalsAntioxidantsApoptosisAspartate AminotransferasesCurcuminEnzyme ActivationEnzyme InhibitorsGene Expression RegulationHSP70 Heat-Shock ProteinsLiverMaleNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIPeroxidaseRatsRats, Sprague-DawleyReperfusion InjuryWarm Ischemia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17461496

Citation

Shen, Shi-Qiang, et al. "Protective Effect of Curcumin Against Liver Warm Ischemia/reperfusion Injury in Rat Model Is Associated With Regulation of Heat Shock Protein and Antioxidant Enzymes." World Journal of Gastroenterology, vol. 13, no. 13, 2007, pp. 1953-61.
Shen SQ, Zhang Y, Xiang JJ, et al. Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes. World J Gastroenterol. 2007;13(13):1953-61.
Shen, S. Q., Zhang, Y., Xiang, J. J., & Xiong, C. L. (2007). Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes. World Journal of Gastroenterology, 13(13), 1953-61.
Shen SQ, et al. Protective Effect of Curcumin Against Liver Warm Ischemia/reperfusion Injury in Rat Model Is Associated With Regulation of Heat Shock Protein and Antioxidant Enzymes. World J Gastroenterol. 2007 Apr 7;13(13):1953-61. PubMed PMID: 17461496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes. AU - Shen,Shi-Qiang, AU - Zhang,Yuan, AU - Xiang,Jin-Jian, AU - Xiong,Cheng-Long, PY - 2007/4/28/pubmed PY - 2007/8/10/medline PY - 2007/4/28/entrez SP - 1953 EP - 61 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 13 IS - 13 N2 - AIM: To investigate the hypothesis that the protective effects of curcumin in hepatic warm ischemia/reperfusion (I/R) injury are associated with increasing heat shock protein 70 (Hsp70) expression and antioxidant enzyme activity. METHODS: Sixty Sprague-Dawley male rats were randomly divided into sham, I/R, C + I/R groups. The model of reduced-size liver warm ischemia and reperfusion was used. Curcumin (50 mg/kg) was administered by injection through a branch of superior mesenteric vein at 30 min before ischemia in C + I/R group. Five rats were used to investigate the survival during 1 wk after operation in each group. Blood samples and liver tissues were obtained in the remaining animals after 3, 12, and 24 h of reperfusion to assess serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver tissue NO(2)(-) + NO(3)(-), malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT), nitricoxide synthase (NOS) and myeloperoxidase (MPO) activity, Hsp70 expression and apoptosis ratio. RESULTS: Compared with I/R group, curcumin pretreatment group showed less ischemia/reperfusion-induced injury. CAT and SOD activity and Hsp70 expression increased significantly. A higher rate of apoptosis was observed in I/R group than in C + I/R group, and a significant increase of MDA, NO(2)(-) + NO(3)(-) and MPO level in liver tissues and serum transaminase concentration was also observed in I/R group compared to C + I/R group. Curcumin also decreased the activity of inducible NO synthase (iNOS) in liver after reperfusion, but had no effect on the level of endothelial NO synthase (eNOS) after reperfusion in liver. The 7 d survival rate was significantly higher in C + I/R group than in I/R group. CONCLUSION: Curcumin has protective effects against hepatic I/R injury. Its mechanism might be related to the overexpression of Hsp70 and antioxidant enzymes. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/17461496/Protective_effect_of_curcumin_against_liver_warm_ischemia/reperfusion_injury_in_rat_model_is_associated_with_regulation_of_heat_shock_protein_and_antioxidant_enzymes_ L2 - https://www.wjgnet.com/1007-9327/full/v13/i13/1953.htm DB - PRIME DP - Unbound Medicine ER -