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[Effects of sex hormones on genioglossal muscle activities, estrogen and androgen receptor expression in adult rat].
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Feb; 42(2):85-9.ZK

Abstract

OBJECTIVE

To explore gene expression of estrogen receptor (ERalpha, ERbeta) and androgen receptor (AR) in genioglossal muscle (GG) of adult male rats, and to investigate the effects of sex hormones on GG activities, ERalpha, ERbeta and AR expression.

METHODS

GG samples were collected from 10 healthy adult male rats. Total RNA were extracted and subjected to fluorescent quantitative reverse transcription-polymerase chain reaction (FQ RT-PCR) for quantitative measurement of ERalpha, ERbeta and AR mRNAs. The other 24 male rats were randomly divided into 3 groups: control group, estrogen group (intramuscular injection of estrogen 0.1 mg/kg, twice a week) and androgen group (intramuscular injection of androgen 2.5 mg/kg, twice a week). The electromyographic activities (EMG) and contract tension of GG were investigated after 4-week treatment. The expression of ERalpha, ERbeta and AR was assessed by Western blot.

RESULTS

The mRNA expression ratios of AR/GAPDH, ERalpha/GAPDH, ERbeta/GAPDH and ERalpha/ERbeta were (295.80 +/- 127.20), (2042.00 +/- 921.57), (65.96 +/- 29.57) and (36.83 +/- 19.66), respectively. The mRNA level of ERalpha was significantly higher than that of ERbeta (P < 0.01). Compared with the control group, the EMG of GG was intensified in the estrogen group (P < 0.01). GG contractility did not change significantly (P > 0.05), and ERalpha expression in GG was up-regulated by estrogen (P < 0.05); while in the androgen group, the EMG of GG was weakened (P < 0.05). P(t) and P(0) were slightly increased (P > 0.05) and the decline rate of P(0) was markedly quickened (P < 0.05). AR and ERbeta expressions were down-regulated by androgen (P < 0.05).

CONCLUSIONS

Both AR and ER were expressed in GG of adult male rats, and ERalpha was expressed more abundantly than ERbeta. Estrogen could greatly improve activities of GG and stimulate the expression of ERalpha. Whereas, androgen could restrain activities of GG, impair its fatigue resistance capacity and inhibit the expression of AR and ERbeta.

Authors+Show Affiliations

Department of Orthodontics, School of Stomatology, Tongji University, Shanghai 200072, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17462152

Citation

Qi, Juan, et al. "[Effects of Sex Hormones On Genioglossal Muscle Activities, Estrogen and Androgen Receptor Expression in Adult Rat]." Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, vol. 42, no. 2, 2007, pp. 85-9.
Qi J, Liu YH, Wang F, et al. [Effects of sex hormones on genioglossal muscle activities, estrogen and androgen receptor expression in adult rat]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2007;42(2):85-9.
Qi, J., Liu, Y. H., Wang, F., Shao, X., & Song, W. H. (2007). [Effects of sex hormones on genioglossal muscle activities, estrogen and androgen receptor expression in adult rat]. Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, 42(2), 85-9.
Qi J, et al. [Effects of Sex Hormones On Genioglossal Muscle Activities, Estrogen and Androgen Receptor Expression in Adult Rat]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2007;42(2):85-9. PubMed PMID: 17462152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of sex hormones on genioglossal muscle activities, estrogen and androgen receptor expression in adult rat]. AU - Qi,Juan, AU - Liu,Yue-hua, AU - Wang,Fei, AU - Shao,Xiao, AU - Song,Wei-hua, PY - 2007/4/28/pubmed PY - 2010/1/6/medline PY - 2007/4/28/entrez SP - 85 EP - 9 JF - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology JO - Zhonghua Kou Qiang Yi Xue Za Zhi VL - 42 IS - 2 N2 - OBJECTIVE: To explore gene expression of estrogen receptor (ERalpha, ERbeta) and androgen receptor (AR) in genioglossal muscle (GG) of adult male rats, and to investigate the effects of sex hormones on GG activities, ERalpha, ERbeta and AR expression. METHODS: GG samples were collected from 10 healthy adult male rats. Total RNA were extracted and subjected to fluorescent quantitative reverse transcription-polymerase chain reaction (FQ RT-PCR) for quantitative measurement of ERalpha, ERbeta and AR mRNAs. The other 24 male rats were randomly divided into 3 groups: control group, estrogen group (intramuscular injection of estrogen 0.1 mg/kg, twice a week) and androgen group (intramuscular injection of androgen 2.5 mg/kg, twice a week). The electromyographic activities (EMG) and contract tension of GG were investigated after 4-week treatment. The expression of ERalpha, ERbeta and AR was assessed by Western blot. RESULTS: The mRNA expression ratios of AR/GAPDH, ERalpha/GAPDH, ERbeta/GAPDH and ERalpha/ERbeta were (295.80 +/- 127.20), (2042.00 +/- 921.57), (65.96 +/- 29.57) and (36.83 +/- 19.66), respectively. The mRNA level of ERalpha was significantly higher than that of ERbeta (P < 0.01). Compared with the control group, the EMG of GG was intensified in the estrogen group (P < 0.01). GG contractility did not change significantly (P > 0.05), and ERalpha expression in GG was up-regulated by estrogen (P < 0.05); while in the androgen group, the EMG of GG was weakened (P < 0.05). P(t) and P(0) were slightly increased (P > 0.05) and the decline rate of P(0) was markedly quickened (P < 0.05). AR and ERbeta expressions were down-regulated by androgen (P < 0.05). CONCLUSIONS: Both AR and ER were expressed in GG of adult male rats, and ERalpha was expressed more abundantly than ERbeta. Estrogen could greatly improve activities of GG and stimulate the expression of ERalpha. Whereas, androgen could restrain activities of GG, impair its fatigue resistance capacity and inhibit the expression of AR and ERbeta. SN - 1002-0098 UR - https://www.unboundmedicine.com/medline/citation/17462152/[Effects_of_sex_hormones_on_genioglossal_muscle_activities_estrogen_and_androgen_receptor_expression_in_adult_rat]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1002-0098&amp;year=2007&amp;vol=42&amp;issue=2&amp;fpage=85 DB - PRIME DP - Unbound Medicine ER -