Tags

Type your tag names separated by a space and hit enter

Therapeutic and prophylactic thalidomide in TNBS-induced colitis: synergistic effects on TNF-alpha, IL-12 and VEGF production.
World J Gastroenterol. 2007 Apr 21; 13(15):2166-73.WJ

Abstract

AIM

To evaluated the therapeutic and prophylactic effect of thalidomide on 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Thalidomide has been reported to downregulate the expression of tumor necrosis factor alpha (TNF-alpha), IL-12, and vascular endothelial growth factor (VEGF), hallmarks of intestinal inflammation in Crohnos disease (CD).

METHODS

Male Wistar rats were divided in five groups of ten animals each. Four groups received a rectal infusion of TNBS in ethanol. The first group was sacrificed 7 d after colitis induction. The second and third groups received either thalidomide or placebo by gavage and were sacrificed at 14 d. The fourth group received thalidomide 6 h before TNBS administration, and was sacrificed 7 d after induction. The fifth group acted as the control group and colitis was not induced. Histological inflammatory scores of the colon were performed and lamina propria CD4+ T cells, macrophages, and VEGF+ cells were detected by immunohistochemistry. TNF-alpha and IL-12 were quantified in the supernatant of organ cultures by ELISA.

RESULTS

Significant reduction in the inflammatory score and in the percentage of VEGF+ cells was observed in the group treated with thalidomide compared with animals not treated with thalidomide. Both TNF-alpha and IL-12 levels were significantly reduced among TNBS induced colitis animals treated with thalidomide compared with animals that did not receive thalidomide. TNF-alpha levels were also significantly reduced among the animals receiving thalidomide prophylaxis compared with untreated animals with TNBS-induced colitis. Intestinal levels of TNF-alpha and IL-12 were significantly correlated with the inflammatory score and the number of VEGF+ cells.

CONCLUSION

Thalidomide significantly attenuates TNBS-induced colitis by inhibiting the intestinal production of TNF-alpha, IL-12, and VEGF. This effect may support the use of thalidomide as an alternate approach in selected patients with CD.

Authors+Show Affiliations

Departamento de Clinica Medica, Hospital Universitario Clementino Fraga Filho, Rua Barao da Torre, 666-101, Ipanema, Rio de Janeiro, RJ, Brazil. atpugas@yahoo.com.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17465495

Citation

Carvalho, Ana Teresa, et al. "Therapeutic and Prophylactic Thalidomide in TNBS-induced Colitis: Synergistic Effects On TNF-alpha, IL-12 and VEGF Production." World Journal of Gastroenterology, vol. 13, no. 15, 2007, pp. 2166-73.
Carvalho AT, Souza H, Carneiro AJ, et al. Therapeutic and prophylactic thalidomide in TNBS-induced colitis: synergistic effects on TNF-alpha, IL-12 and VEGF production. World J Gastroenterol. 2007;13(15):2166-73.
Carvalho, A. T., Souza, H., Carneiro, A. J., Castelo-Branco, M., Madi, K., Schanaider, A., Silv, F., Pereira Junior, F. A., Pereira, M. G., Tortori, C., Dines, I., Carvalho, J., Rocha, E., & Elia, C. (2007). Therapeutic and prophylactic thalidomide in TNBS-induced colitis: synergistic effects on TNF-alpha, IL-12 and VEGF production. World Journal of Gastroenterology, 13(15), 2166-73.
Carvalho AT, et al. Therapeutic and Prophylactic Thalidomide in TNBS-induced Colitis: Synergistic Effects On TNF-alpha, IL-12 and VEGF Production. World J Gastroenterol. 2007 Apr 21;13(15):2166-73. PubMed PMID: 17465495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic and prophylactic thalidomide in TNBS-induced colitis: synergistic effects on TNF-alpha, IL-12 and VEGF production. AU - Carvalho,Ana Teresa, AU - Souza,Heitor, AU - Carneiro,Antonio Jose, AU - Castelo-Branco,Morgana, AU - Madi,Kalil, AU - Schanaider,Alberto, AU - Silv,Flavia, AU - Pereira Junior,Fernando Antonio, AU - Pereira,Marcia G, AU - Tortori,Claudio, AU - Dines,Ilana, AU - Carvalho,Jane, AU - Rocha,Eduardo, AU - Elia,Celeste, PY - 2007/5/1/pubmed PY - 2007/9/26/medline PY - 2007/5/1/entrez SP - 2166 EP - 73 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 13 IS - 15 N2 - AIM: To evaluated the therapeutic and prophylactic effect of thalidomide on 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Thalidomide has been reported to downregulate the expression of tumor necrosis factor alpha (TNF-alpha), IL-12, and vascular endothelial growth factor (VEGF), hallmarks of intestinal inflammation in Crohnos disease (CD). METHODS: Male Wistar rats were divided in five groups of ten animals each. Four groups received a rectal infusion of TNBS in ethanol. The first group was sacrificed 7 d after colitis induction. The second and third groups received either thalidomide or placebo by gavage and were sacrificed at 14 d. The fourth group received thalidomide 6 h before TNBS administration, and was sacrificed 7 d after induction. The fifth group acted as the control group and colitis was not induced. Histological inflammatory scores of the colon were performed and lamina propria CD4+ T cells, macrophages, and VEGF+ cells were detected by immunohistochemistry. TNF-alpha and IL-12 were quantified in the supernatant of organ cultures by ELISA. RESULTS: Significant reduction in the inflammatory score and in the percentage of VEGF+ cells was observed in the group treated with thalidomide compared with animals not treated with thalidomide. Both TNF-alpha and IL-12 levels were significantly reduced among TNBS induced colitis animals treated with thalidomide compared with animals that did not receive thalidomide. TNF-alpha levels were also significantly reduced among the animals receiving thalidomide prophylaxis compared with untreated animals with TNBS-induced colitis. Intestinal levels of TNF-alpha and IL-12 were significantly correlated with the inflammatory score and the number of VEGF+ cells. CONCLUSION: Thalidomide significantly attenuates TNBS-induced colitis by inhibiting the intestinal production of TNF-alpha, IL-12, and VEGF. This effect may support the use of thalidomide as an alternate approach in selected patients with CD. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/17465495/Therapeutic_and_prophylactic_thalidomide_in_TNBS_induced_colitis:_synergistic_effects_on_TNF_alpha_IL_12_and_VEGF_production_ L2 - http://www.wjgnet.com/1007-9327/full/v13/i15/2166.htm DB - PRIME DP - Unbound Medicine ER -