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Suppressive effects of cannabidiol on antigen-specific antibody production and functional activity of splenocytes in ovalbumin-sensitized BALB/c mice.
Int Immunopharmacol. 2007 Jun; 7(6):773-80.II

Abstract

Cannabidiol (CBD) and cannabis-based medicines are potential therapeutic agents. Because the immune system has been widely demonstrated to be affected by psychoactive cannabinoids, such as Delta(9)-tetrahydrocannabinol, the objective of the present studies is to investigate the immunomodulatory effect of CBD, the major non-psychoactive cannabinoid in marijuana. BALB/c mice were intraperitoneally administered with a single dose of CBD (5-20 mg/kg) prior to ovalbumin (OVA) sensitization, and the serum production of antigen-specific antibodies was measured 7 days post OVA sensitization. The serum level of OVA-specific IgM was significantly attenuated by a high dose of CBD (20 mg/kg), and OVA-specific IgG(1) and IgG(2a) by all 3 doses of CBD. Concordantly, splenocytes of mice administered with CBD (5 or 20 mg/kg) produced less IL-2, IL-4 and IFN-gamma than those of vehicle-treated controls, upon ex vivo stimulation with phorbol ester plus calcium ionophore. Likewise, T-cell mitogen (concanavalin A)-induced proliferation of splenocytes was also markedly suppressed in mice administered with CBD. Furthermore, the observed ex vivo effects of CBD on cytokine production and T-cell proliferation were confirmed in splenocytes directly exposed to CBD (1-8 microM) in vitro, indicating a direct effect by CBD. Taken together, the results demonstrated that CBD markedly suppressed antigen-specific antibody production in OVA-sensitized mice, and suggest that CBD-mediated suppression of humoral immunity could be mediated by the impaired functions of splenocytes.

Authors+Show Affiliations

Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC. tonyjan@ntu.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17466911

Citation

Jan, Tong-Rong, et al. "Suppressive Effects of Cannabidiol On Antigen-specific Antibody Production and Functional Activity of Splenocytes in Ovalbumin-sensitized BALB/c Mice." International Immunopharmacology, vol. 7, no. 6, 2007, pp. 773-80.
Jan TR, Su ST, Wu HY, et al. Suppressive effects of cannabidiol on antigen-specific antibody production and functional activity of splenocytes in ovalbumin-sensitized BALB/c mice. Int Immunopharmacol. 2007;7(6):773-80.
Jan, T. R., Su, S. T., Wu, H. Y., & Liao, M. H. (2007). Suppressive effects of cannabidiol on antigen-specific antibody production and functional activity of splenocytes in ovalbumin-sensitized BALB/c mice. International Immunopharmacology, 7(6), 773-80.
Jan TR, et al. Suppressive Effects of Cannabidiol On Antigen-specific Antibody Production and Functional Activity of Splenocytes in Ovalbumin-sensitized BALB/c Mice. Int Immunopharmacol. 2007;7(6):773-80. PubMed PMID: 17466911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppressive effects of cannabidiol on antigen-specific antibody production and functional activity of splenocytes in ovalbumin-sensitized BALB/c mice. AU - Jan,Tong-Rong, AU - Su,Shu-Ting, AU - Wu,Hsin-Ying, AU - Liao,Mei-Hsiu, Y1 - 2007/02/15/ PY - 2006/12/13/received PY - 2007/01/15/revised PY - 2007/01/15/accepted PY - 2007/5/1/pubmed PY - 2007/8/7/medline PY - 2007/5/1/entrez SP - 773 EP - 80 JF - International immunopharmacology JO - Int Immunopharmacol VL - 7 IS - 6 N2 - Cannabidiol (CBD) and cannabis-based medicines are potential therapeutic agents. Because the immune system has been widely demonstrated to be affected by psychoactive cannabinoids, such as Delta(9)-tetrahydrocannabinol, the objective of the present studies is to investigate the immunomodulatory effect of CBD, the major non-psychoactive cannabinoid in marijuana. BALB/c mice were intraperitoneally administered with a single dose of CBD (5-20 mg/kg) prior to ovalbumin (OVA) sensitization, and the serum production of antigen-specific antibodies was measured 7 days post OVA sensitization. The serum level of OVA-specific IgM was significantly attenuated by a high dose of CBD (20 mg/kg), and OVA-specific IgG(1) and IgG(2a) by all 3 doses of CBD. Concordantly, splenocytes of mice administered with CBD (5 or 20 mg/kg) produced less IL-2, IL-4 and IFN-gamma than those of vehicle-treated controls, upon ex vivo stimulation with phorbol ester plus calcium ionophore. Likewise, T-cell mitogen (concanavalin A)-induced proliferation of splenocytes was also markedly suppressed in mice administered with CBD. Furthermore, the observed ex vivo effects of CBD on cytokine production and T-cell proliferation were confirmed in splenocytes directly exposed to CBD (1-8 microM) in vitro, indicating a direct effect by CBD. Taken together, the results demonstrated that CBD markedly suppressed antigen-specific antibody production in OVA-sensitized mice, and suggest that CBD-mediated suppression of humoral immunity could be mediated by the impaired functions of splenocytes. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/17466911/Suppressive_effects_of_cannabidiol_on_antigen_specific_antibody_production_and_functional_activity_of_splenocytes_in_ovalbumin_sensitized_BALB/c_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(07)00035-5 DB - PRIME DP - Unbound Medicine ER -