TY - JOUR T1 - Genetic and biochemical characterization of GES-5, an extended-spectrum class A beta-lactamase from Klebsiella pneumoniae. AU - Bae,Il Kwon, AU - Lee,You-Nae, AU - Jeong,Seok Hoon, AU - Hong,Seong Geun, AU - Lee,Jung Hun, AU - Lee,Sang Hee, AU - Kim,Hyoung Jin, AU - Youn,Hasik, Y1 - 2007/04/27/ PY - 2006/12/26/received PY - 2007/02/19/revised PY - 2007/02/25/accepted PY - 2007/5/1/pubmed PY - 2007/10/24/medline PY - 2007/5/1/entrez SP - 465 EP - 8 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 58 IS - 4 N2 - The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 beta-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6')-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most beta-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/17467221/Genetic_and_biochemical_characterization_of_GES_5_an_extended_spectrum_class_A_beta_lactamase_from_Klebsiella_pneumoniae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(07)00103-4 DB - PRIME DP - Unbound Medicine ER -