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Cross-neutralisation of Australian brown and tiger snake venoms with commercial antivenoms: Cross-reactivity or antivenom mixtures?
Toxicon. 2007 Aug; 50(2):206-13.T

Abstract

Recently it has been suggested that the Australian snake antivenoms made by CSL Ltd. are in fact not truly monovalent and may contain antibodies to other snake venoms because the horses are injected with multiple snake venoms. It is unclear to what extent various monovalent antivenoms can neutralise the effect of other venoms, whether this is due to a mixture of antibodies or true cross-reactivity, and whether this has any clinical significance. We aimed to study the immunological and functional properties of brown snake (Pseudonaja spp.) antivenom (BSAV) and tiger snake (Notechis spp.) antivenom (TSAV) against their respective venoms using enzyme immunoassays (EIA) and in vitro clotting studies. There was significant overlap between the two antivenoms with both TSAV and BSAV being detected by EIA on brown snake venom (BSV)-coated and tiger snake venom (TSV)-coated wells, respectively. In a competition EIA, increasing amounts of immunoaffinity-purified hen anti-brown antibodies (IgYp) mixed with TSAV reduced TSAV measured on TSV-coated wells. Both BSAV and TSAV prevented the clotting activity of both venoms. IgYp also prevented the clotting activity of TSV, suggesting true cross-reactivity. The cross-reactivity of TSAV and BSAV with BSV and TSV, respectively, was likely due to each being a mixture of anti-brown and anti-tiger antibodies, but there was partial cross-reactivity demonstrated by the effect of IgYp. Single-polyvalent antivenom for brown snake and tiger snake may be feasible in the future.

Authors+Show Affiliations

Department of Clinical Toxicology and Pharmacology, Newcastle Mater Hospital, Newcastle, NSW, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17467763

Citation

O'Leary, Margaret A., et al. "Cross-neutralisation of Australian Brown and Tiger Snake Venoms With Commercial Antivenoms: Cross-reactivity or Antivenom Mixtures?" Toxicon : Official Journal of the International Society On Toxinology, vol. 50, no. 2, 2007, pp. 206-13.
O'Leary MA, Schneider JJ, Krishnan BP, et al. Cross-neutralisation of Australian brown and tiger snake venoms with commercial antivenoms: Cross-reactivity or antivenom mixtures? Toxicon. 2007;50(2):206-13.
O'Leary, M. A., Schneider, J. J., Krishnan, B. P., Lavis, C., McKendry, A., Ong, L. K., & Isbister, G. K. (2007). Cross-neutralisation of Australian brown and tiger snake venoms with commercial antivenoms: Cross-reactivity or antivenom mixtures? Toxicon : Official Journal of the International Society On Toxinology, 50(2), 206-13.
O'Leary MA, et al. Cross-neutralisation of Australian Brown and Tiger Snake Venoms With Commercial Antivenoms: Cross-reactivity or Antivenom Mixtures. Toxicon. 2007;50(2):206-13. PubMed PMID: 17467763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-neutralisation of Australian brown and tiger snake venoms with commercial antivenoms: Cross-reactivity or antivenom mixtures? AU - O'Leary,Margaret A, AU - Schneider,Jennifer J, AU - Krishnan,Brian Prakash, AU - Lavis,Christopher, AU - McKendry,Amy, AU - Ong,Lin Kooi, AU - Isbister,Geoffrey K, Y1 - 2007/03/28/ PY - 2007/01/09/received PY - 2007/03/12/revised PY - 2007/03/15/accepted PY - 2007/5/1/pubmed PY - 2007/10/4/medline PY - 2007/5/1/entrez SP - 206 EP - 13 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 50 IS - 2 N2 - Recently it has been suggested that the Australian snake antivenoms made by CSL Ltd. are in fact not truly monovalent and may contain antibodies to other snake venoms because the horses are injected with multiple snake venoms. It is unclear to what extent various monovalent antivenoms can neutralise the effect of other venoms, whether this is due to a mixture of antibodies or true cross-reactivity, and whether this has any clinical significance. We aimed to study the immunological and functional properties of brown snake (Pseudonaja spp.) antivenom (BSAV) and tiger snake (Notechis spp.) antivenom (TSAV) against their respective venoms using enzyme immunoassays (EIA) and in vitro clotting studies. There was significant overlap between the two antivenoms with both TSAV and BSAV being detected by EIA on brown snake venom (BSV)-coated and tiger snake venom (TSV)-coated wells, respectively. In a competition EIA, increasing amounts of immunoaffinity-purified hen anti-brown antibodies (IgYp) mixed with TSAV reduced TSAV measured on TSV-coated wells. Both BSAV and TSAV prevented the clotting activity of both venoms. IgYp also prevented the clotting activity of TSV, suggesting true cross-reactivity. The cross-reactivity of TSAV and BSAV with BSV and TSV, respectively, was likely due to each being a mixture of anti-brown and anti-tiger antibodies, but there was partial cross-reactivity demonstrated by the effect of IgYp. Single-polyvalent antivenom for brown snake and tiger snake may be feasible in the future. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/17467763/Cross_neutralisation_of_Australian_brown_and_tiger_snake_venoms_with_commercial_antivenoms:_Cross_reactivity_or_antivenom_mixtures L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(07)00104-3 DB - PRIME DP - Unbound Medicine ER -