Tags

Type your tag names separated by a space and hit enter

Tissue response to subcutaneous implantation of glucose-oxidase-based glucose sensors in rats.
Biosens Bioelectron. 2007 Aug 30; 23(1):26-34.BB

Abstract

Considerable progress in improved control of disturbed glucose metabolism can be expected by continuous glucose monitoring. The aim of the study was to evaluate in male Sprague-Dawley rats tissue response to implantation of a new amperometric glucose-oxidase-based glucose sensor (NTS) compared to a commercially available sensor system CGMS of MiniMed. Both sensors were tested under working conditions over a period of 3 days. Using NTS, glucose in interstitial fluid reflected glucose in arterial blood even in rapidly changing hyper- and hypoglycaemia whereas the CGMS did not detect the experimentally induced glucose changes adequately. Gene expression profiling was performed using Affymetrix chips. Acute phase response to injury by sensor application for a short time is indicated by down regulation of the increase in mRNA of proteases e.g. metallothionein-1alpha and matrix metalloprotease-3 at day 3. Improvement of anabolic situation is suggested by decrease in mRNA of insulin-like growth factor binding protein whereas increase of heme oxygenase and hypoxia-inducible factor may be related to defense mechanisms. Changes of mRNA together with slight fibrous capsule formation suggest good histocompatibility. Comparability of the patterns of changed mRNA in tissue surrounding SCGM with and without operating voltage as shown in dendrogram indicates no contribution of hydrogen peroxide to worsening biocompatibility. Beside established histological investigations of foreign body reaction weeks or months after implantation, gene expression profiling provides additional information to biocompatibility already early after implantation.

Authors+Show Affiliations

Medical Research Center, University Hospital Mannheim, University of Heidelberg, D68167 Mannheim, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17467971

Citation

Henninger, Nadja, et al. "Tissue Response to Subcutaneous Implantation of Glucose-oxidase-based Glucose Sensors in Rats." Biosensors & Bioelectronics, vol. 23, no. 1, 2007, pp. 26-34.
Henninger N, Woderer S, Kloetzer HM, et al. Tissue response to subcutaneous implantation of glucose-oxidase-based glucose sensors in rats. Biosens Bioelectron. 2007;23(1):26-34.
Henninger, N., Woderer, S., Kloetzer, H. M., Staib, A., Gillen, R., Li, L., Yu, X., Gretz, N., Kraenzlin, B., & Pill, J. (2007). Tissue response to subcutaneous implantation of glucose-oxidase-based glucose sensors in rats. Biosensors & Bioelectronics, 23(1), 26-34.
Henninger N, et al. Tissue Response to Subcutaneous Implantation of Glucose-oxidase-based Glucose Sensors in Rats. Biosens Bioelectron. 2007 Aug 30;23(1):26-34. PubMed PMID: 17467971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue response to subcutaneous implantation of glucose-oxidase-based glucose sensors in rats. AU - Henninger,Nadja, AU - Woderer,Stefanie, AU - Kloetzer,Hans-Martin, AU - Staib,Arnulf, AU - Gillen,Ralph, AU - Li,Li, AU - Yu,Xiaolei, AU - Gretz,Norbert, AU - Kraenzlin,Bettina, AU - Pill,Johannes, Y1 - 2007/03/30/ PY - 2006/12/21/received PY - 2007/02/21/revised PY - 2007/03/19/accepted PY - 2007/5/1/pubmed PY - 2007/12/6/medline PY - 2007/5/1/entrez SP - 26 EP - 34 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 1 N2 - Considerable progress in improved control of disturbed glucose metabolism can be expected by continuous glucose monitoring. The aim of the study was to evaluate in male Sprague-Dawley rats tissue response to implantation of a new amperometric glucose-oxidase-based glucose sensor (NTS) compared to a commercially available sensor system CGMS of MiniMed. Both sensors were tested under working conditions over a period of 3 days. Using NTS, glucose in interstitial fluid reflected glucose in arterial blood even in rapidly changing hyper- and hypoglycaemia whereas the CGMS did not detect the experimentally induced glucose changes adequately. Gene expression profiling was performed using Affymetrix chips. Acute phase response to injury by sensor application for a short time is indicated by down regulation of the increase in mRNA of proteases e.g. metallothionein-1alpha and matrix metalloprotease-3 at day 3. Improvement of anabolic situation is suggested by decrease in mRNA of insulin-like growth factor binding protein whereas increase of heme oxygenase and hypoxia-inducible factor may be related to defense mechanisms. Changes of mRNA together with slight fibrous capsule formation suggest good histocompatibility. Comparability of the patterns of changed mRNA in tissue surrounding SCGM with and without operating voltage as shown in dendrogram indicates no contribution of hydrogen peroxide to worsening biocompatibility. Beside established histological investigations of foreign body reaction weeks or months after implantation, gene expression profiling provides additional information to biocompatibility already early after implantation. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17467971/Tissue_response_to_subcutaneous_implantation_of_glucose_oxidase_based_glucose_sensors_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00174-1 DB - PRIME DP - Unbound Medicine ER -