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Stable oil bodies sheltered by a unique oleosin in lily pollen.
Plant Cell Physiol. 2007 Jun; 48(6):812-21.PC

Abstract

Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. The integrity of pollen oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies and massively accumulated in late stages of pollen maturation. According to mass spectrometric analyses, this oil body protein possessed a tryptic fragment of 13 residues matching that of a theoretical rice oleosin. A complete cDNA fragment encoding this putative oleosin was obtained by PCR cloning with primers derived from its known 13-residue sequence. Sequence analysis as well as immunological non-cross-reactivity suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. In pollen cells observed by electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route from that of glyoxysomal mobilization of seed oil bodies after germination.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17468126

Citation

Jiang, Pei-Luen, et al. "Stable Oil Bodies Sheltered By a Unique Oleosin in Lily Pollen." Plant & Cell Physiology, vol. 48, no. 6, 2007, pp. 812-21.
Jiang PL, Wang CS, Hsu CM, et al. Stable oil bodies sheltered by a unique oleosin in lily pollen. Plant Cell Physiol. 2007;48(6):812-21.
Jiang, P. L., Wang, C. S., Hsu, C. M., Jauh, G. Y., & Tzen, J. T. (2007). Stable oil bodies sheltered by a unique oleosin in lily pollen. Plant & Cell Physiology, 48(6), 812-21.
Jiang PL, et al. Stable Oil Bodies Sheltered By a Unique Oleosin in Lily Pollen. Plant Cell Physiol. 2007;48(6):812-21. PubMed PMID: 17468126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stable oil bodies sheltered by a unique oleosin in lily pollen. AU - Jiang,Pei-Luen, AU - Wang,Co-Shing, AU - Hsu,Chia-Mei, AU - Jauh,Guang-Yuh, AU - Tzen,Jason T C, Y1 - 2007/04/27/ PY - 2007/5/1/pubmed PY - 2007/10/12/medline PY - 2007/5/1/entrez SP - 812 EP - 21 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 48 IS - 6 N2 - Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. The integrity of pollen oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies and massively accumulated in late stages of pollen maturation. According to mass spectrometric analyses, this oil body protein possessed a tryptic fragment of 13 residues matching that of a theoretical rice oleosin. A complete cDNA fragment encoding this putative oleosin was obtained by PCR cloning with primers derived from its known 13-residue sequence. Sequence analysis as well as immunological non-cross-reactivity suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. In pollen cells observed by electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route from that of glyoxysomal mobilization of seed oil bodies after germination. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/17468126/Stable_oil_bodies_sheltered_by_a_unique_oleosin_in_lily_pollen_ DB - PRIME DP - Unbound Medicine ER -