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Sensitive determination of MDMA and its metabolite MDA in rat blood and brain microdialysates by HPLC with fluorescence detection.
Biomed Chromatogr. 2007 Oct; 21(10):1016-22.BC

Abstract

Simultaneous determination of 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in rat blood and brain microdialysates by high-performance liquid chromatography with fluorescence detection (HPLC-FL) was developed. Microdialysates were directly subjected to derivatization with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl). The DIB-derivatives of MDMA, MDA and the internal standard, 1-methyl-3-phenylpropylamine (MPPA), were isocratically separated on an ODS column using a mixture of 50 mm phosphate buffer (pH 7.0)-acetonitrile-methanol-2-propanol (50:45:5:2, v/v/v/v %) as an eluent at a flow rate of 1.5 mL/min. The calibration curves of MDA and MDMA spiked to blood and brain microdialysates were linear over the ranges 2.5-500 and 5.0-1000 ng/mL, respectively. The detection limits of MDA and MDMA were 1.2 and 4.2 for blood and 1.3 and 4.8 ng/mL for brain, respectively. Additionally, the intra- and the inter-assay precisions were lower than 5.6% for the blood and brain microdialysates (n = 4). The proposed method was successfully applied for the monitoring of MDMA and its metabolite MDA in rat blood and brain microdialysates, and the pharmacokinetic parameters of MDMA and MDA in the microdialysates after administration of MDMA (5 mg/kg, i.p.) with or without caffeine (20 mg/kg, i.p.) were evaluated.

Authors+Show Affiliations

Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17474141

Citation

Tomita, Mamoru, et al. "Sensitive Determination of MDMA and Its Metabolite MDA in Rat Blood and Brain Microdialysates By HPLC With Fluorescence Detection." Biomedical Chromatography : BMC, vol. 21, no. 10, 2007, pp. 1016-22.
Tomita M, Nakashima MN, Wada M, et al. Sensitive determination of MDMA and its metabolite MDA in rat blood and brain microdialysates by HPLC with fluorescence detection. Biomed Chromatogr. 2007;21(10):1016-22.
Tomita, M., Nakashima, M. N., Wada, M., & Nakashima, K. (2007). Sensitive determination of MDMA and its metabolite MDA in rat blood and brain microdialysates by HPLC with fluorescence detection. Biomedical Chromatography : BMC, 21(10), 1016-22.
Tomita M, et al. Sensitive Determination of MDMA and Its Metabolite MDA in Rat Blood and Brain Microdialysates By HPLC With Fluorescence Detection. Biomed Chromatogr. 2007;21(10):1016-22. PubMed PMID: 17474141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive determination of MDMA and its metabolite MDA in rat blood and brain microdialysates by HPLC with fluorescence detection. AU - Tomita,Mamoru, AU - Nakashima,Mihoko N, AU - Wada,Mitsuhiro, AU - Nakashima,Kenichiro, PY - 2007/5/3/pubmed PY - 2007/12/21/medline PY - 2007/5/3/entrez SP - 1016 EP - 22 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 21 IS - 10 N2 - Simultaneous determination of 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in rat blood and brain microdialysates by high-performance liquid chromatography with fluorescence detection (HPLC-FL) was developed. Microdialysates were directly subjected to derivatization with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl). The DIB-derivatives of MDMA, MDA and the internal standard, 1-methyl-3-phenylpropylamine (MPPA), were isocratically separated on an ODS column using a mixture of 50 mm phosphate buffer (pH 7.0)-acetonitrile-methanol-2-propanol (50:45:5:2, v/v/v/v %) as an eluent at a flow rate of 1.5 mL/min. The calibration curves of MDA and MDMA spiked to blood and brain microdialysates were linear over the ranges 2.5-500 and 5.0-1000 ng/mL, respectively. The detection limits of MDA and MDMA were 1.2 and 4.2 for blood and 1.3 and 4.8 ng/mL for brain, respectively. Additionally, the intra- and the inter-assay precisions were lower than 5.6% for the blood and brain microdialysates (n = 4). The proposed method was successfully applied for the monitoring of MDMA and its metabolite MDA in rat blood and brain microdialysates, and the pharmacokinetic parameters of MDMA and MDA in the microdialysates after administration of MDMA (5 mg/kg, i.p.) with or without caffeine (20 mg/kg, i.p.) were evaluated. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/17474141/Sensitive_determination_of_MDMA_and_its_metabolite_MDA_in_rat_blood_and_brain_microdialysates_by_HPLC_with_fluorescence_detection_ L2 - https://doi.org/10.1002/bmc.839 DB - PRIME DP - Unbound Medicine ER -