Tags

Type your tag names separated by a space and hit enter

Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography.
Biomed Chromatogr. 2007 Sep; 21(9):987-92.BC

Abstract

A micellar electrokinetic capillary chromatography (MECC) method has been developed for the determination of the four isoflavones, i.e. biochanin A, formononetin, genstein and daidzein in red clover (Trifolium Pratense L.). The effect of running buffer pH and concentration were investigated. An electrolyte composed of 30 mm borate, 20 mm sodium dodecyl sulfate (SDS) and 4 mg/mL HP-beta-CD containing 5% (v/v) ethanol at pH 10.1 provides a satisfactory separation for all the analytes. The applied voltage was 25 kV, and the capillary temperature was kept constant at 25 degrees C with a UV detection at 254 nm. The relative standard deviations (RSD) of the migration time and peak area were less than 1.73 and 3.94% (intra-day), and 2.29 and 4.38% (inter-day), respectively, under the optimized separation conditions. Regression equations revealed a good linear relationship between the peak area of each compound and its concentration. The contents of the four compounds in red clover were successfully determined with satisfactory repeatability and recovery.

Authors+Show Affiliations

Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17474156

Citation

Zhang, Yu, et al. "Separation and Determination of Isoflavones in Red Clover By Micellar Electrokinetic Capillary Chromatography." Biomedical Chromatography : BMC, vol. 21, no. 9, 2007, pp. 987-92.
Zhang Y, Chen J, Zhao L, et al. Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography. Biomed Chromatogr. 2007;21(9):987-92.
Zhang, Y., Chen, J., Zhao, L., & Shi, Y. P. (2007). Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography. Biomedical Chromatography : BMC, 21(9), 987-92.
Zhang Y, et al. Separation and Determination of Isoflavones in Red Clover By Micellar Electrokinetic Capillary Chromatography. Biomed Chromatogr. 2007;21(9):987-92. PubMed PMID: 17474156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography. AU - Zhang,Yu, AU - Chen,Juan, AU - Zhao,Liang, AU - Shi,Yan-Ping, PY - 2007/5/3/pubmed PY - 2007/11/8/medline PY - 2007/5/3/entrez SP - 987 EP - 92 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 21 IS - 9 N2 - A micellar electrokinetic capillary chromatography (MECC) method has been developed for the determination of the four isoflavones, i.e. biochanin A, formononetin, genstein and daidzein in red clover (Trifolium Pratense L.). The effect of running buffer pH and concentration were investigated. An electrolyte composed of 30 mm borate, 20 mm sodium dodecyl sulfate (SDS) and 4 mg/mL HP-beta-CD containing 5% (v/v) ethanol at pH 10.1 provides a satisfactory separation for all the analytes. The applied voltage was 25 kV, and the capillary temperature was kept constant at 25 degrees C with a UV detection at 254 nm. The relative standard deviations (RSD) of the migration time and peak area were less than 1.73 and 3.94% (intra-day), and 2.29 and 4.38% (inter-day), respectively, under the optimized separation conditions. Regression equations revealed a good linear relationship between the peak area of each compound and its concentration. The contents of the four compounds in red clover were successfully determined with satisfactory repeatability and recovery. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/17474156/Separation_and_determination_of_isoflavones_in_red_clover_by_micellar_electrokinetic_capillary_chromatography_ L2 - https://doi.org/10.1002/bmc.843 DB - PRIME DP - Unbound Medicine ER -