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Cytoprotective properties of phenolic antidiarrheic ingredients in cultured astrocytes and neurons of rat brains.
Eur J Pharmacol. 2007 Jul 12; 567(1-2):59-66.EJ

Abstract

We have previously shown that particular phenolic antidiarrheic ingredients, including 2-methoxy-4-methylphenol (2M4MP) and 2-methoxy-4-ethyphenol (2M4EP), but not 2-methoxyphenol (2MP), significantly inhibit cellular maturation and differentiation of the bone-resorbing osteoclasts with concomitant protection of the bone-forming osteoblasts against oxidative stress by hydrogen peroxide (H(2)O(2)). In the present study, we evaluated the pharmacological actions of these three major phenolic antidiarrheic ingredients on the cellular viability in cultured astrocytes and neurons of the rat brain in vitro. Both 2M4MP and 2M4EP induced more efficient prevention of cell death induced by the brief exposure to 0.1 mM H(2)O(2) for 2 h than 2MP upon simultaneous exposure in cultured rat cortical astrocytes. Similarly, both 2M4MP and 2M4EP were more effective than 2MP in significantly protecting the cytotoxicity by brief exposure to 0.1 mM H(2)O(2) for 6 h in cultured rat hippocampal neurons, with concomitant suppression of the generation of intracellular reactive oxygen species in neurons exposed to H(2)O(2). Moreover, the three ingredients not only significantly prevented cell death in hippocampal neurons exposed to 0.1 mM glutamate for 1 h when determined 48 h after the brief exposure, but also inhibited the generation of intracellular reactive oxygen species and the elevation of intracellular Ca(2+) ions in neurons exposed to glutamate. These results suggest that particular phenolic antidiarrheic ingredients may prevent cell death through a mechanism related to diminution of the neurotoxicity of glutamate in neurons, in addition to eliciting cytoprotection against oxidative stress in astrocytes and neurons, in the rat brain.

Authors+Show Affiliations

Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kanazawa, Ishikawa 920-1192, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17475240

Citation

Matsushima, Nobuyuki, et al. "Cytoprotective Properties of Phenolic Antidiarrheic Ingredients in Cultured Astrocytes and Neurons of Rat Brains." European Journal of Pharmacology, vol. 567, no. 1-2, 2007, pp. 59-66.
Matsushima N, Nakamichi N, Kambe Y, et al. Cytoprotective properties of phenolic antidiarrheic ingredients in cultured astrocytes and neurons of rat brains. Eur J Pharmacol. 2007;567(1-2):59-66.
Matsushima, N., Nakamichi, N., Kambe, Y., Takano, K., Moriguchi, N., & Yoneda, Y. (2007). Cytoprotective properties of phenolic antidiarrheic ingredients in cultured astrocytes and neurons of rat brains. European Journal of Pharmacology, 567(1-2), 59-66.
Matsushima N, et al. Cytoprotective Properties of Phenolic Antidiarrheic Ingredients in Cultured Astrocytes and Neurons of Rat Brains. Eur J Pharmacol. 2007 Jul 12;567(1-2):59-66. PubMed PMID: 17475240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytoprotective properties of phenolic antidiarrheic ingredients in cultured astrocytes and neurons of rat brains. AU - Matsushima,Nobuyuki, AU - Nakamichi,Noritaka, AU - Kambe,Yuki, AU - Takano,Katsura, AU - Moriguchi,Nobuaki, AU - Yoneda,Yukio, Y1 - 2007/03/30/ PY - 2006/10/21/received PY - 2007/03/06/revised PY - 2007/03/08/accepted PY - 2007/5/4/pubmed PY - 2007/7/28/medline PY - 2007/5/4/entrez SP - 59 EP - 66 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 567 IS - 1-2 N2 - We have previously shown that particular phenolic antidiarrheic ingredients, including 2-methoxy-4-methylphenol (2M4MP) and 2-methoxy-4-ethyphenol (2M4EP), but not 2-methoxyphenol (2MP), significantly inhibit cellular maturation and differentiation of the bone-resorbing osteoclasts with concomitant protection of the bone-forming osteoblasts against oxidative stress by hydrogen peroxide (H(2)O(2)). In the present study, we evaluated the pharmacological actions of these three major phenolic antidiarrheic ingredients on the cellular viability in cultured astrocytes and neurons of the rat brain in vitro. Both 2M4MP and 2M4EP induced more efficient prevention of cell death induced by the brief exposure to 0.1 mM H(2)O(2) for 2 h than 2MP upon simultaneous exposure in cultured rat cortical astrocytes. Similarly, both 2M4MP and 2M4EP were more effective than 2MP in significantly protecting the cytotoxicity by brief exposure to 0.1 mM H(2)O(2) for 6 h in cultured rat hippocampal neurons, with concomitant suppression of the generation of intracellular reactive oxygen species in neurons exposed to H(2)O(2). Moreover, the three ingredients not only significantly prevented cell death in hippocampal neurons exposed to 0.1 mM glutamate for 1 h when determined 48 h after the brief exposure, but also inhibited the generation of intracellular reactive oxygen species and the elevation of intracellular Ca(2+) ions in neurons exposed to glutamate. These results suggest that particular phenolic antidiarrheic ingredients may prevent cell death through a mechanism related to diminution of the neurotoxicity of glutamate in neurons, in addition to eliciting cytoprotection against oxidative stress in astrocytes and neurons, in the rat brain. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/17475240/Cytoprotective_properties_of_phenolic_antidiarrheic_ingredients_in_cultured_astrocytes_and_neurons_of_rat_brains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)00354-8 DB - PRIME DP - Unbound Medicine ER -