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Role of phosphatidylinositol-4-phosphate 5' kinase (ppk-1) in ovulation of Caenorhabditis elegans.
Exp Cell Res. 2007 Jul 01; 313(11):2465-75.EC

Abstract

During Caenorhabditis elegans ovulation, the somatic gonad integrates signals from germ cells and propels a mature oocyte into the spermatheca for fertilization. Previous work suggests that phosphoinositide signaling plays important roles in C. elegans fertility. To fully understand inositol-1,4,5-trisphosphate (IP(3)) signaling in ovulation, we have examined the function of phosphatidylinositol-4-phosphate 5' kinase (PIP5K) in C. elegans. Our results show that the C. elegans PIP5K homolog, ppk-1, is essential for ovulation in C. elegans; ppk-1 is mainly expressed in somatic gonad, and depletion of ppk-1 expression causes defective ovulation, reduced gonad sheath contractility, and sterility. Increased IP(3) signaling compensates for ppk-1 (RNAi)-induced sterility, suggesting that ppk-1 is linked to IP(3) signaling. These results demonstrate that ppk-1 plays an essential role in IP(3) signaling and cytoskeleton organization in somatic gonad.

Authors+Show Affiliations

Department of Biology, Baylor University, One Bear Place 97388, Waco, TX 76798, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17475243

Citation

Xu, Xiaojian, et al. "Role of Phosphatidylinositol-4-phosphate 5' Kinase (ppk-1) in Ovulation of Caenorhabditis Elegans." Experimental Cell Research, vol. 313, no. 11, 2007, pp. 2465-75.
Xu X, Guo H, Wycuff DL, et al. Role of phosphatidylinositol-4-phosphate 5' kinase (ppk-1) in ovulation of Caenorhabditis elegans. Exp Cell Res. 2007;313(11):2465-75.
Xu, X., Guo, H., Wycuff, D. L., & Lee, M. (2007). Role of phosphatidylinositol-4-phosphate 5' kinase (ppk-1) in ovulation of Caenorhabditis elegans. Experimental Cell Research, 313(11), 2465-75.
Xu X, et al. Role of Phosphatidylinositol-4-phosphate 5' Kinase (ppk-1) in Ovulation of Caenorhabditis Elegans. Exp Cell Res. 2007 Jul 1;313(11):2465-75. PubMed PMID: 17475243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of phosphatidylinositol-4-phosphate 5' kinase (ppk-1) in ovulation of Caenorhabditis elegans. AU - Xu,Xiaojian, AU - Guo,Haisu, AU - Wycuff,Diane L, AU - Lee,Myeongwoo, Y1 - 2007/03/24/ PY - 2006/11/15/received PY - 2007/03/15/revised PY - 2007/03/19/accepted PY - 2007/5/4/pubmed PY - 2007/8/19/medline PY - 2007/5/4/entrez SP - 2465 EP - 75 JF - Experimental cell research JO - Exp Cell Res VL - 313 IS - 11 N2 - During Caenorhabditis elegans ovulation, the somatic gonad integrates signals from germ cells and propels a mature oocyte into the spermatheca for fertilization. Previous work suggests that phosphoinositide signaling plays important roles in C. elegans fertility. To fully understand inositol-1,4,5-trisphosphate (IP(3)) signaling in ovulation, we have examined the function of phosphatidylinositol-4-phosphate 5' kinase (PIP5K) in C. elegans. Our results show that the C. elegans PIP5K homolog, ppk-1, is essential for ovulation in C. elegans; ppk-1 is mainly expressed in somatic gonad, and depletion of ppk-1 expression causes defective ovulation, reduced gonad sheath contractility, and sterility. Increased IP(3) signaling compensates for ppk-1 (RNAi)-induced sterility, suggesting that ppk-1 is linked to IP(3) signaling. These results demonstrate that ppk-1 plays an essential role in IP(3) signaling and cytoskeleton organization in somatic gonad. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/17475243/Role_of_phosphatidylinositol_4_phosphate_5'_kinase__ppk_1__in_ovulation_of_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(07)00138-3 DB - PRIME DP - Unbound Medicine ER -