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In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens.

Abstract

Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 microg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 microg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC-MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases.

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  • Authors+Show Affiliations

    ,

    Department of Microbiology, Division of Medical Parasitology, Gulhane Military Medical Academy School of Medicine, 06018 Ankara, Turkey. okoru@gata.edu.tr

    , , , , , , , , ,

    Source

    Anaerobe 13:3-4 pg 140-5

    MeSH

    Animals
    Anti-Bacterial Agents
    Bees
    Flavonoids
    Gram-Negative Bacteria
    Gram-Positive Bacteria
    Microbial Sensitivity Tests
    Mouth Diseases
    Propolis

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    17475517

    Citation

    Koru, Ozgur, et al. "In Vitro Antimicrobial Activity of Propolis Samples From Different Geographical Origins Against Certain Oral Pathogens." Anaerobe, vol. 13, no. 3-4, 2007, pp. 140-5.
    Koru O, Toksoy F, Acikel CH, et al. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 2007;13(3-4):140-5.
    Koru, O., Toksoy, F., Acikel, C. H., Tunca, Y. M., Baysallar, M., Uskudar Guclu, A., ... Salih, B. (2007). In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe, 13(3-4), pp. 140-5.
    Koru O, et al. In Vitro Antimicrobial Activity of Propolis Samples From Different Geographical Origins Against Certain Oral Pathogens. Anaerobe. 2007;13(3-4):140-5. PubMed PMID: 17475517.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. AU - Koru,Ozgur, AU - Toksoy,Fulya, AU - Acikel,Cengiz Han, AU - Tunca,Yasar Meric, AU - Baysallar,Mehmet, AU - Uskudar Guclu,Aylin, AU - Akca,Eralp, AU - Ozkok Tuylu,Asli, AU - Sorkun,Kadriye, AU - Tanyuksel,Mehmet, AU - Salih,Bekir, Y1 - 2007/03/07/ PY - 2006/04/10/received PY - 2006/09/08/revised PY - 2007/02/23/accepted PY - 2007/5/4/pubmed PY - 2007/12/7/medline PY - 2007/5/4/entrez SP - 140 EP - 5 JF - Anaerobe JO - Anaerobe VL - 13 IS - 3-4 N2 - Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 microg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 microg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC-MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases. SN - 1075-9964 UR - https://www.unboundmedicine.com/medline/citation/17475517/In_vitro_antimicrobial_activity_of_propolis_samples_from_different_geographical_origins_against_certain_oral_pathogens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(07)00017-0 DB - PRIME DP - Unbound Medicine ER -