Tags

Type your tag names separated by a space and hit enter

Alpha-lipoic acid differently affects the reserpine-induced oxidative stress in the striatum and prefrontal cortex of rat brain.
Neuroscience. 2007 Jun 08; 146(4):1758-71.N

Abstract

Antioxidative properties of alpha-lipoic acid (LA) are widely investigated in different in vivo and in vitro models. The aim of this study was to examine whether LA attenuates oxidative stress induced in rats by reserpine, a model substance frequently used to produce Parkinsonism in animals. Male Wistar rats were treated with reserpine (5 mg/kg) and LA (50 mg/kg) separately or in combination. The levels of reduced glutathione (GSH), glutathione disulfide (GSSG), nitric oxide (NO) and S-nitrosothiols as well as activities of glutathione peroxidase (GPx), glutathione-S-transferase (GST) and L-gamma-glutamyl transpeptidase (gamma-GT) were determined in the striatum and prefrontal cortex homogenates. In the striatum and prefrontal cortex a single dose of reserpine significantly enhanced levels of GSSG and NO but not that of S-nitrosothiols when compared with control. In the striatum, LA administered jointly with reserpine markedly increased the concentration of GSH and decreased GSSG level. In the prefrontal cortex, such treatment produced only an increasing tendency in GSH level but caused no changes in GSSG content. In both structures LA injected jointly with reserpine markedly decreased NO concentrations but did not cause significant changes in S-nitrosothiol levels when compared with control. Enzymatic activities of GPx and GST were intensified by LA in the striatum. In the prefrontal cortex, GPx activity was not altered, while that of GST was decreased. Gamma-GT activity was attenuated by reserpine in the striatum while LA reversed this effect. Such changes were not observed in the prefrontal cortex. The mode of LA action in the striatum during the reserpine-evoked oxidative stress strongly suggests that this compound may be of therapeutic value in the treatment of Parkinson's disease.

Authors+Show Affiliations

Collegium Medicum, Jagiellonian University, 7, Kopernika Street, PL-31-034 Kraków, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17478054

Citation

Bilska, A, et al. "Alpha-lipoic Acid Differently Affects the Reserpine-induced Oxidative Stress in the Striatum and Prefrontal Cortex of Rat Brain." Neuroscience, vol. 146, no. 4, 2007, pp. 1758-71.
Bilska A, Dubiel M, Sokołowska-Jezewicz M, et al. Alpha-lipoic acid differently affects the reserpine-induced oxidative stress in the striatum and prefrontal cortex of rat brain. Neuroscience. 2007;146(4):1758-71.
Bilska, A., Dubiel, M., Sokołowska-Jezewicz, M., Lorenc-Koci, E., & Włodek, L. (2007). Alpha-lipoic acid differently affects the reserpine-induced oxidative stress in the striatum and prefrontal cortex of rat brain. Neuroscience, 146(4), 1758-71.
Bilska A, et al. Alpha-lipoic Acid Differently Affects the Reserpine-induced Oxidative Stress in the Striatum and Prefrontal Cortex of Rat Brain. Neuroscience. 2007 Jun 8;146(4):1758-71. PubMed PMID: 17478054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alpha-lipoic acid differently affects the reserpine-induced oxidative stress in the striatum and prefrontal cortex of rat brain. AU - Bilska,A, AU - Dubiel,M, AU - Sokołowska-Jezewicz,M, AU - Lorenc-Koci,E, AU - Włodek,L, Y1 - 2007/05/02/ PY - 2006/12/20/received PY - 2007/03/23/revised PY - 2007/04/01/accepted PY - 2007/5/5/pubmed PY - 2007/10/27/medline PY - 2007/5/5/entrez SP - 1758 EP - 71 JF - Neuroscience JO - Neuroscience VL - 146 IS - 4 N2 - Antioxidative properties of alpha-lipoic acid (LA) are widely investigated in different in vivo and in vitro models. The aim of this study was to examine whether LA attenuates oxidative stress induced in rats by reserpine, a model substance frequently used to produce Parkinsonism in animals. Male Wistar rats were treated with reserpine (5 mg/kg) and LA (50 mg/kg) separately or in combination. The levels of reduced glutathione (GSH), glutathione disulfide (GSSG), nitric oxide (NO) and S-nitrosothiols as well as activities of glutathione peroxidase (GPx), glutathione-S-transferase (GST) and L-gamma-glutamyl transpeptidase (gamma-GT) were determined in the striatum and prefrontal cortex homogenates. In the striatum and prefrontal cortex a single dose of reserpine significantly enhanced levels of GSSG and NO but not that of S-nitrosothiols when compared with control. In the striatum, LA administered jointly with reserpine markedly increased the concentration of GSH and decreased GSSG level. In the prefrontal cortex, such treatment produced only an increasing tendency in GSH level but caused no changes in GSSG content. In both structures LA injected jointly with reserpine markedly decreased NO concentrations but did not cause significant changes in S-nitrosothiol levels when compared with control. Enzymatic activities of GPx and GST were intensified by LA in the striatum. In the prefrontal cortex, GPx activity was not altered, while that of GST was decreased. Gamma-GT activity was attenuated by reserpine in the striatum while LA reversed this effect. Such changes were not observed in the prefrontal cortex. The mode of LA action in the striatum during the reserpine-evoked oxidative stress strongly suggests that this compound may be of therapeutic value in the treatment of Parkinson's disease. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/17478054/Alpha_lipoic_acid_differently_affects_the_reserpine_induced_oxidative_stress_in_the_striatum_and_prefrontal_cortex_of_rat_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(07)00460-5 DB - PRIME DP - Unbound Medicine ER -