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Visceral and somatic hypersensitivity in a subset of rats following TNBS-induced colitis.
Pain 2008; 134(1-2):9-15PAIN

Abstract

BACKGROUND

Chronic abdominal pain is one of the most common gastrointestinal symptoms experienced by patients. Visceral hypersensitivity has been shown to be a biological marker in many patients with chronic visceral pain. We have previously shown that IBS patients with visceral hypersensitivity also have evidence of thermal hyperalgesia of the hand/foot.

OBJECTIVE

The objective of the current study was to develop an animal model of chronic visceral and somatic hypersensitivity in rats treated with intracolonic trinitrobenzene sulfonic acid.

DESIGN

Male Sprague-Dawley rats (200-250g) were treated with either 20mg/rat trinitrobenzene sulfonic acid (TNBS, Sigma Chemical Co.) in 50% ethanol (n=75), an equivalent volume of 50% ethanol (n=20) or an equivalent volume of saline (n=20). The agents were delivered with a 24-gauge catheter inserted into the lumen of the colon. Mechanical and thermal behavioral tests were performed using an automated von Frey and Hargreaves device to evaluate somatic hyperalgesia. Colonic distension was performed using an automated distension device to evaluate visceral pain thresholds. All animals were tested 16weeks after TNBS treatment following complete resolution of the colitis.

RESULTS

At 16weeks, 24% of the treated rats (18/75 rats) still exhibited evidence of visceral as well as somatic hypersensitivity compared to saline- and ethanol-treated rats.

CONCLUSION

Transient colonic inflammation leads to chronic visceral and somatic hypersensitivity in a subset of rats. These findings are similar to the subset of patients who develop chronic gastrointestinal symptoms following enteric infection.

Authors+Show Affiliations

Department of Medicine, Division of Gasteroenterology, Hepatology, and Nutrition, University of Florida Colleges of Medicine and Dentistry, 1600 SW Archer Road, HD 602, PO Box 100214, Gainesville, FL 32610-0214, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17481818

Citation

Zhou, QiQi, et al. "Visceral and Somatic Hypersensitivity in a Subset of Rats Following TNBS-induced Colitis." Pain, vol. 134, no. 1-2, 2008, pp. 9-15.
Zhou Q, Price DD, Caudle RM, et al. Visceral and somatic hypersensitivity in a subset of rats following TNBS-induced colitis. Pain. 2008;134(1-2):9-15.
Zhou, Q., Price, D. D., Caudle, R. M., & Verne, G. N. (2008). Visceral and somatic hypersensitivity in a subset of rats following TNBS-induced colitis. Pain, 134(1-2), pp. 9-15.
Zhou Q, et al. Visceral and Somatic Hypersensitivity in a Subset of Rats Following TNBS-induced Colitis. Pain. 2008;134(1-2):9-15. PubMed PMID: 17481818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visceral and somatic hypersensitivity in a subset of rats following TNBS-induced colitis. AU - Zhou,QiQi, AU - Price,Donald D, AU - Caudle,Robert M, AU - Verne,G Nicholas, Y1 - 2007/05/03/ PY - 2007/01/16/received PY - 2007/02/19/revised PY - 2007/03/19/accepted PY - 2007/5/8/pubmed PY - 2008/3/11/medline PY - 2007/5/8/entrez SP - 9 EP - 15 JF - Pain JO - Pain VL - 134 IS - 1-2 N2 - BACKGROUND: Chronic abdominal pain is one of the most common gastrointestinal symptoms experienced by patients. Visceral hypersensitivity has been shown to be a biological marker in many patients with chronic visceral pain. We have previously shown that IBS patients with visceral hypersensitivity also have evidence of thermal hyperalgesia of the hand/foot. OBJECTIVE: The objective of the current study was to develop an animal model of chronic visceral and somatic hypersensitivity in rats treated with intracolonic trinitrobenzene sulfonic acid. DESIGN: Male Sprague-Dawley rats (200-250g) were treated with either 20mg/rat trinitrobenzene sulfonic acid (TNBS, Sigma Chemical Co.) in 50% ethanol (n=75), an equivalent volume of 50% ethanol (n=20) or an equivalent volume of saline (n=20). The agents were delivered with a 24-gauge catheter inserted into the lumen of the colon. Mechanical and thermal behavioral tests were performed using an automated von Frey and Hargreaves device to evaluate somatic hyperalgesia. Colonic distension was performed using an automated distension device to evaluate visceral pain thresholds. All animals were tested 16weeks after TNBS treatment following complete resolution of the colitis. RESULTS: At 16weeks, 24% of the treated rats (18/75 rats) still exhibited evidence of visceral as well as somatic hypersensitivity compared to saline- and ethanol-treated rats. CONCLUSION: Transient colonic inflammation leads to chronic visceral and somatic hypersensitivity in a subset of rats. These findings are similar to the subset of patients who develop chronic gastrointestinal symptoms following enteric infection. SN - 1872-6623 UR - https://www.unboundmedicine.com/medline/citation/17481818/Visceral_and_somatic_hypersensitivity_in_a_subset_of_rats_following_TNBS_induced_colitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3959(07)00136-4 DB - PRIME DP - Unbound Medicine ER -