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Stereoselective determination of vigabatrin enantiomers in human plasma by high performance liquid chromatography using UV detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 01; 854(1-2):63-7.JC

Abstract

A rapid and simple high-performance liquid chromatographic method for the determination of the R-(-)- and S-(+)-enantiomers of the antiepileptic drug vigabatrin in human plasma is described. After adding the internal standard (1-aminomethyl-cycloheptyl-acetic acid), plasma samples (200 microL) are deproteinized with acetonitrile and the supernatant is derivatized with 2,4,6 trinitrobenzene sulfonic acid (TNBSA). Separation is achieved on a reversed-phase cellulose-based chiral column (Chiralcel-ODR, 250 mm x 4.6 mm i.d.) using 0.05 M potassium hexafluorophosphate (pH 4.5)/acetonitrile/ethanol (50:40:10 vol/vol/vol) as mobile phase at a flow-rate of 0.9 mL/min. Chromatographic selectivity is improved by concentrating the derivatives on High Performance Extraction Disk Cartridges prior to injection. Detection is at 340 nm. Calibration curves are linear (r(2)> or =0.999) over the range of 0.5-40 microg/mL for each enantiomer, with a limit of quantification of 0.5 microg/mL for both analytes. The assay is suitable for therapeutic drug monitoring and for single-dose pharmacokinetic studies in man.

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Authors+Show Affiliations

Franco V
Clinical Pharmacology Unit, Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy.
Mazzucchelli I
No affiliation info available
Fattore C
No affiliation info available
Marchiselli R
No affiliation info available
Gatti G
No affiliation info available
Perucca E
No affiliation info available

MeSH

CalibrationChromatography, High Pressure LiquidHumansReference StandardsSensitivity and SpecificitySpectrophotometry, UltravioletStereoisomerismVigabatrin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17481975

Citation

Franco, Valentina, et al. "Stereoselective Determination of Vigabatrin Enantiomers in Human Plasma By High Performance Liquid Chromatography Using UV Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 854, no. 1-2, 2007, pp. 63-7.
Franco V, Mazzucchelli I, Fattore C, et al. Stereoselective determination of vigabatrin enantiomers in human plasma by high performance liquid chromatography using UV detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;854(1-2):63-7.
Franco, V., Mazzucchelli, I., Fattore, C., Marchiselli, R., Gatti, G., & Perucca, E. (2007). Stereoselective determination of vigabatrin enantiomers in human plasma by high performance liquid chromatography using UV detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 854(1-2), 63-7.
Franco V, et al. Stereoselective Determination of Vigabatrin Enantiomers in Human Plasma By High Performance Liquid Chromatography Using UV Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):63-7. PubMed PMID: 17481975.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stereoselective determination of vigabatrin enantiomers in human plasma by high performance liquid chromatography using UV detection. AU - Franco,Valentina, AU - Mazzucchelli,Iolanda, AU - Fattore,Cinzia, AU - Marchiselli,Roberto, AU - Gatti,Giuliana, AU - Perucca,Emilio, Y1 - 2007/04/08/ PY - 2006/11/28/received PY - 2007/03/19/revised PY - 2007/03/30/accepted PY - 2007/5/8/pubmed PY - 2007/10/10/medline PY - 2007/5/8/entrez SP - 63 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 854 IS - 1-2 N2 - A rapid and simple high-performance liquid chromatographic method for the determination of the R-(-)- and S-(+)-enantiomers of the antiepileptic drug vigabatrin in human plasma is described. After adding the internal standard (1-aminomethyl-cycloheptyl-acetic acid), plasma samples (200 microL) are deproteinized with acetonitrile and the supernatant is derivatized with 2,4,6 trinitrobenzene sulfonic acid (TNBSA). Separation is achieved on a reversed-phase cellulose-based chiral column (Chiralcel-ODR, 250 mm x 4.6 mm i.d.) using 0.05 M potassium hexafluorophosphate (pH 4.5)/acetonitrile/ethanol (50:40:10 vol/vol/vol) as mobile phase at a flow-rate of 0.9 mL/min. Chromatographic selectivity is improved by concentrating the derivatives on High Performance Extraction Disk Cartridges prior to injection. Detection is at 340 nm. Calibration curves are linear (r(2)> or =0.999) over the range of 0.5-40 microg/mL for each enantiomer, with a limit of quantification of 0.5 microg/mL for both analytes. The assay is suitable for therapeutic drug monitoring and for single-dose pharmacokinetic studies in man. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17481975/Stereoselective_determination_of_vigabatrin_enantiomers_in_human_plasma_by_high_performance_liquid_chromatography_using_UV_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00263-2 DB - PRIME DP - Unbound Medicine ER -