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Monitoring cell adhesion by using thickness shear mode acoustic wave sensors.
Biosens Bioelectron. 2007 Aug 30; 23(1):42-50.BB

Abstract

The thickness shear mode (TSM) acoustic wave sensor attached with living cells has been shown to be an effective functional biosensing device to monitor the process of cell adhesion to a surface in real time. In this study, a multilayer sensor model that includes a quartz substrate, a cell-substrate interfacial layer and a cell layer was constructed based on the state of cell adhesion to the substrate. The dynamic process of cell adhesions as a function of cell seeding densities was monitored using the acoustic wave sensor. The mechanisms that are responsible for the frequency and resistance change are discussed according to the predictions of the acoustic wave sensor model. In addition, knowing that the actin cytoskeleton is important for cell adhesion, we investigated the motional resistance change caused by the disruption of actin cytoskeleton induced by fungal toxin cytochalasin D in the human skin fibroblasts. The results indicate that resistance changes are related to the disruption of actin cytoskeleton and dosage-dependent.

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Authors+Show Affiliations

Li F
Department of Mechanical Engineering and Material Science, University of Pittsburgh, Pittsburgh, PA 15261, United States.
Wang JH
No affiliation info available
Wang QM
No affiliation info available

MeSH

AcousticsAdhesivenessBiosensing TechniquesCell AdhesionCell Culture TechniquesCells, CulturedComputer-Aided DesignEquipment DesignEquipment Failure AnalysisFibroblastsHumansShear StrengthStress, Mechanical

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17485202

Citation

Li, Fang, et al. "Monitoring Cell Adhesion By Using Thickness Shear Mode Acoustic Wave Sensors." Biosensors & Bioelectronics, vol. 23, no. 1, 2007, pp. 42-50.
Li F, Wang JH, Wang QM. Monitoring cell adhesion by using thickness shear mode acoustic wave sensors. Biosens Bioelectron. 2007;23(1):42-50.
Li, F., Wang, J. H., & Wang, Q. M. (2007). Monitoring cell adhesion by using thickness shear mode acoustic wave sensors. Biosensors & Bioelectronics, 23(1), 42-50.
Li F, Wang JH, Wang QM. Monitoring Cell Adhesion By Using Thickness Shear Mode Acoustic Wave Sensors. Biosens Bioelectron. 2007 Aug 30;23(1):42-50. PubMed PMID: 17485202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring cell adhesion by using thickness shear mode acoustic wave sensors. AU - Li,Fang, AU - Wang,James H-C, AU - Wang,Qing-Ming, Y1 - 2007/03/30/ PY - 2006/12/12/received PY - 2007/02/16/revised PY - 2007/03/19/accepted PY - 2007/5/9/pubmed PY - 2007/12/6/medline PY - 2007/5/9/entrez SP - 42 EP - 50 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 1 N2 - The thickness shear mode (TSM) acoustic wave sensor attached with living cells has been shown to be an effective functional biosensing device to monitor the process of cell adhesion to a surface in real time. In this study, a multilayer sensor model that includes a quartz substrate, a cell-substrate interfacial layer and a cell layer was constructed based on the state of cell adhesion to the substrate. The dynamic process of cell adhesions as a function of cell seeding densities was monitored using the acoustic wave sensor. The mechanisms that are responsible for the frequency and resistance change are discussed according to the predictions of the acoustic wave sensor model. In addition, knowing that the actin cytoskeleton is important for cell adhesion, we investigated the motional resistance change caused by the disruption of actin cytoskeleton induced by fungal toxin cytochalasin D in the human skin fibroblasts. The results indicate that resistance changes are related to the disruption of actin cytoskeleton and dosage-dependent. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17485202/Monitoring_cell_adhesion_by_using_thickness_shear_mode_acoustic_wave_sensors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00177-7 DB - PRIME DP - Unbound Medicine ER -