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Cytologic diagnosis of Ewing sarcoma/peripheral neuroectodermal tumor with paired prospective molecular genetic analysis.
Cancer. 2007 Jun 25; 111(3):192-9.C

Abstract

BACKGROUND

Ewing sarcoma/peripheral neuroectodermal tumor (EWS/PNET), since its characterization immunophenotypically and cytogenetically, has emerged as one of most common sarcomas of childhood. Currently, it is recognized that EWS/PNET can occur in any number of extraosseous sites and is one of several distinctive tumor types with an EWS translocation. In the past, the pathologic diagnosis of EWS/PNET relied on an open biopsy with the application of various ancillary studies, ranging from periodic acid-Schiff stain to molecular testing, but the tumor increasingly is diagnosed on the basis of cytologic specimens alone.

METHODS

The authors report 3 cases of EWS/PNET in patients aged 11 years to 53 years. These 3 patients had tumors that involved the parotid gland, cervical soft tissue, and pelvis, and were diagnosed by cytologic evaluation of fine-needle aspiration (FNA) biopsy material alone. The FNA materials also were evaluated prospectively by fluorescence in situ hybridization and/or reverse transcriptase-polymerase chain reaction.

RESULTS

The results emphasized the diagnostic utility of FNA biopsy material for morphologic and molecular analysis without compromising conventional cytologic and immunocytochemical analysis, and that prospective molecular testing of FNA specimens has utility in routine practice although it is subject to many of the same limitations that impact molecular analysis when applied to conventional tissue biopsy specimens.

CONCLUSIONS

The current results demonstrated that molecular genetic techniques can provide clinically useful ancillary information for FNA specimens when cytologic features and/or immunophenotype are equivocal on the basis of limited sampling or secondary changes, such as hemorrhage and/or necrosis.

Authors+Show Affiliations

Lauren V Ackerman Laboratory of Surgical Pathology, Barnes-Jewish and Saint Louis Children's Hospitals, Washington University Medical Center, St Louis, MO 63110-1093, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

17487851

Citation

Sanati, Souzan, et al. "Cytologic Diagnosis of Ewing Sarcoma/peripheral Neuroectodermal Tumor With Paired Prospective Molecular Genetic Analysis." Cancer, vol. 111, no. 3, 2007, pp. 192-9.
Sanati S, Lu DW, Schmidt E, et al. Cytologic diagnosis of Ewing sarcoma/peripheral neuroectodermal tumor with paired prospective molecular genetic analysis. Cancer. 2007;111(3):192-9.
Sanati, S., Lu, D. W., Schmidt, E., Perry, A., Dehner, L. P., & Pfeifer, J. D. (2007). Cytologic diagnosis of Ewing sarcoma/peripheral neuroectodermal tumor with paired prospective molecular genetic analysis. Cancer, 111(3), 192-9.
Sanati S, et al. Cytologic Diagnosis of Ewing Sarcoma/peripheral Neuroectodermal Tumor With Paired Prospective Molecular Genetic Analysis. Cancer. 2007 Jun 25;111(3):192-9. PubMed PMID: 17487851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytologic diagnosis of Ewing sarcoma/peripheral neuroectodermal tumor with paired prospective molecular genetic analysis. AU - Sanati,Souzan, AU - Lu,Danielle W, AU - Schmidt,Edith, AU - Perry,Arie, AU - Dehner,Louis P, AU - Pfeifer,John D, PY - 2007/5/10/pubmed PY - 2007/7/17/medline PY - 2007/5/10/entrez SP - 192 EP - 9 JF - Cancer JO - Cancer VL - 111 IS - 3 N2 - BACKGROUND: Ewing sarcoma/peripheral neuroectodermal tumor (EWS/PNET), since its characterization immunophenotypically and cytogenetically, has emerged as one of most common sarcomas of childhood. Currently, it is recognized that EWS/PNET can occur in any number of extraosseous sites and is one of several distinctive tumor types with an EWS translocation. In the past, the pathologic diagnosis of EWS/PNET relied on an open biopsy with the application of various ancillary studies, ranging from periodic acid-Schiff stain to molecular testing, but the tumor increasingly is diagnosed on the basis of cytologic specimens alone. METHODS: The authors report 3 cases of EWS/PNET in patients aged 11 years to 53 years. These 3 patients had tumors that involved the parotid gland, cervical soft tissue, and pelvis, and were diagnosed by cytologic evaluation of fine-needle aspiration (FNA) biopsy material alone. The FNA materials also were evaluated prospectively by fluorescence in situ hybridization and/or reverse transcriptase-polymerase chain reaction. RESULTS: The results emphasized the diagnostic utility of FNA biopsy material for morphologic and molecular analysis without compromising conventional cytologic and immunocytochemical analysis, and that prospective molecular testing of FNA specimens has utility in routine practice although it is subject to many of the same limitations that impact molecular analysis when applied to conventional tissue biopsy specimens. CONCLUSIONS: The current results demonstrated that molecular genetic techniques can provide clinically useful ancillary information for FNA specimens when cytologic features and/or immunophenotype are equivocal on the basis of limited sampling or secondary changes, such as hemorrhage and/or necrosis. SN - 0008-543X UR - https://www.unboundmedicine.com/medline/citation/17487851/Cytologic_diagnosis_of_Ewing_sarcoma/peripheral_neuroectodermal_tumor_with_paired_prospective_molecular_genetic_analysis_ L2 - https://doi.org/10.1002/cncr.22692 DB - PRIME DP - Unbound Medicine ER -