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Increased fat oxidation and regulation of metabolic genes with ultraendurance exercise.
Acta Physiol (Oxf). 2007 Sep; 191(1):77-86.AP

Abstract

AIM

Regular endurance exercise stimulates muscle metabolic capacity, but effects of very prolonged endurance exercise are largely unknown. This study examined muscle substrate availability and utilization during prolonged endurance exercise, and associated metabolic genes.

METHODS

Data were obtained from 11 competitors of a 4- to 5-day, almost continuous ultraendurance race (seven males, four females; age: 36 +/- 11 years; cycling Vo(2peak): males 57.4 +/- 5.9, females 48.1 +/- 4.0 mL kg(-1) min(-1)). Before and after the race muscle biopsies were obtained from vastus lateralis, respiratory gases were sampled during cycling at 25 and 50% peak aerobic power output, venous samples were obtained, and fat mass was estimated by bioimpedance under standardized conditions.

RESULTS

After the race fat mass was decreased by 1.6 +/- 0.4 kg (11%; P < 0.01). Respiratory exchange ratio at the 25 and 50% workloads decreased (P < 0.01) from 0.83 +/- 0.06 and 0.93 +/- 0.03 before, to 0.71 +/- 0.01 and 0.85 +/- 0.02, respectively, after the race. Plasma fatty acids were 3.5 times higher (from 298 +/- 74 to 1407 +/- 118 micromol L(-1); P < 0.01). Muscle glycogen content fell 50% (from 554 +/- 28 to 270 +/- 25 nmol kg(-1) d.w.; n = 7, P < 0.01), whereas the decline in muscle triacylglycerol (from 32 +/- 5 to 22 +/- 3 mmol kg(-1) d.w.; P = 0.14) was not statistically significant. After the race, muscle mRNA content of lipoprotein lipase and glycogen synthase increased (P < 0.05) 3.9- and 1.7-fold, respectively, while forkhead homolog in rhabdomyosarcoma, pyruvate dehydrogenase kinase 4 and vascular endothelial growth factor mRNA tended (P < 0.10) to be higher, whereas muscle peroxisome proliferator-activated receptor gamma co-activator-1beta mRNA tended to be lower (P = 0.06).

CONCLUSION

Very prolonged exercise markedly increases plasma fatty acid availability and fat utilization during exercise. Exercise-induced regulation of genes encoding proteins involved in fatty acid recruitment and oxidation may contribute to these changes.

Authors+Show Affiliations

Department of Biomedical Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark. jhelge@mfi.ku.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17488246

Citation

Helge, J W., et al. "Increased Fat Oxidation and Regulation of Metabolic Genes With Ultraendurance Exercise." Acta Physiologica (Oxford, England), vol. 191, no. 1, 2007, pp. 77-86.
Helge JW, Rehrer NJ, Pilegaard H, et al. Increased fat oxidation and regulation of metabolic genes with ultraendurance exercise. Acta Physiol (Oxf). 2007;191(1):77-86.
Helge, J. W., Rehrer, N. J., Pilegaard, H., Manning, P., Lucas, S. J., Gerrard, D. F., & Cotter, J. D. (2007). Increased fat oxidation and regulation of metabolic genes with ultraendurance exercise. Acta Physiologica (Oxford, England), 191(1), 77-86.
Helge JW, et al. Increased Fat Oxidation and Regulation of Metabolic Genes With Ultraendurance Exercise. Acta Physiol (Oxf). 2007;191(1):77-86. PubMed PMID: 17488246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased fat oxidation and regulation of metabolic genes with ultraendurance exercise. AU - Helge,J W, AU - Rehrer,N J, AU - Pilegaard,H, AU - Manning,P, AU - Lucas,S J E, AU - Gerrard,D F, AU - Cotter,J D, Y1 - 2007/05/03/ PY - 2007/5/10/pubmed PY - 2007/12/11/medline PY - 2007/5/10/entrez SP - 77 EP - 86 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 191 IS - 1 N2 - AIM: Regular endurance exercise stimulates muscle metabolic capacity, but effects of very prolonged endurance exercise are largely unknown. This study examined muscle substrate availability and utilization during prolonged endurance exercise, and associated metabolic genes. METHODS: Data were obtained from 11 competitors of a 4- to 5-day, almost continuous ultraendurance race (seven males, four females; age: 36 +/- 11 years; cycling Vo(2peak): males 57.4 +/- 5.9, females 48.1 +/- 4.0 mL kg(-1) min(-1)). Before and after the race muscle biopsies were obtained from vastus lateralis, respiratory gases were sampled during cycling at 25 and 50% peak aerobic power output, venous samples were obtained, and fat mass was estimated by bioimpedance under standardized conditions. RESULTS: After the race fat mass was decreased by 1.6 +/- 0.4 kg (11%; P < 0.01). Respiratory exchange ratio at the 25 and 50% workloads decreased (P < 0.01) from 0.83 +/- 0.06 and 0.93 +/- 0.03 before, to 0.71 +/- 0.01 and 0.85 +/- 0.02, respectively, after the race. Plasma fatty acids were 3.5 times higher (from 298 +/- 74 to 1407 +/- 118 micromol L(-1); P < 0.01). Muscle glycogen content fell 50% (from 554 +/- 28 to 270 +/- 25 nmol kg(-1) d.w.; n = 7, P < 0.01), whereas the decline in muscle triacylglycerol (from 32 +/- 5 to 22 +/- 3 mmol kg(-1) d.w.; P = 0.14) was not statistically significant. After the race, muscle mRNA content of lipoprotein lipase and glycogen synthase increased (P < 0.05) 3.9- and 1.7-fold, respectively, while forkhead homolog in rhabdomyosarcoma, pyruvate dehydrogenase kinase 4 and vascular endothelial growth factor mRNA tended (P < 0.10) to be higher, whereas muscle peroxisome proliferator-activated receptor gamma co-activator-1beta mRNA tended to be lower (P = 0.06). CONCLUSION: Very prolonged exercise markedly increases plasma fatty acid availability and fat utilization during exercise. Exercise-induced regulation of genes encoding proteins involved in fatty acid recruitment and oxidation may contribute to these changes. SN - 1748-1708 UR - https://www.unboundmedicine.com/medline/citation/17488246/Increased_fat_oxidation_and_regulation_of_metabolic_genes_with_ultraendurance_exercise_ L2 - https://doi.org/10.1111/j.1748-1716.2007.01709.x DB - PRIME DP - Unbound Medicine ER -