Tags

Type your tag names separated by a space and hit enter

Calcium induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha and mitochondrial biogenesis by a pathway leading to p38 mitogen-activated protein kinase activation.
J Biol Chem. 2007 Jun 29; 282(26):18793-9.JB

Abstract

Previous studies have shown that raising cytosolic calcium in myotubes induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis. This finding suggests that the increases in cytosolic calcium in skeletal muscle during exercise may mediate the exercise-induced increase in mitochondria. The initial aim of this study was to determine whether raising calcium in skeletal muscle induces the same adaptations as in myotubes. We found that treatment of rat epitrochlearis muscles with a concentration of caffeine that raises cytosolic calcium to a concentration too low to cause contraction induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis. Our second aim was to elucidate the pathway by which calcium induces these adaptations. Raising cytosolic calcium has been shown to activate calcium/calmodulin-dependent protein kinase in muscle. In the present study raising cytosolic calcium resulted in increases in phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor-2, which were blocked by the calcium/calmodulin-dependent protein kinase inhibitor KN93 and by the p38 mitogen-activated protein kinase inhibitor SB202190. The increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis were also prevented by inhibiting p38 activation. We interpret these findings as evidence that p38 mitogen-activated protein kinase is downstream of calcium/calmodulin-dependent protein kinase in a signaling pathway by which increases in cytosolic calcium lead to increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis in muscle.

Authors+Show Affiliations

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17488713

Citation

Wright, David C., et al. "Calcium Induces Increases in Peroxisome Proliferator-activated Receptor Gamma Coactivator-1alpha and Mitochondrial Biogenesis By a Pathway Leading to P38 Mitogen-activated Protein Kinase Activation." The Journal of Biological Chemistry, vol. 282, no. 26, 2007, pp. 18793-9.
Wright DC, Geiger PC, Han DH, et al. Calcium induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha and mitochondrial biogenesis by a pathway leading to p38 mitogen-activated protein kinase activation. J Biol Chem. 2007;282(26):18793-9.
Wright, D. C., Geiger, P. C., Han, D. H., Jones, T. E., & Holloszy, J. O. (2007). Calcium induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha and mitochondrial biogenesis by a pathway leading to p38 mitogen-activated protein kinase activation. The Journal of Biological Chemistry, 282(26), 18793-9.
Wright DC, et al. Calcium Induces Increases in Peroxisome Proliferator-activated Receptor Gamma Coactivator-1alpha and Mitochondrial Biogenesis By a Pathway Leading to P38 Mitogen-activated Protein Kinase Activation. J Biol Chem. 2007 Jun 29;282(26):18793-9. PubMed PMID: 17488713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calcium induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha and mitochondrial biogenesis by a pathway leading to p38 mitogen-activated protein kinase activation. AU - Wright,David C, AU - Geiger,Paige C, AU - Han,Dong-Ho, AU - Jones,Terry E, AU - Holloszy,John O, Y1 - 2007/05/07/ PY - 2007/5/10/pubmed PY - 2007/9/14/medline PY - 2007/5/10/entrez SP - 18793 EP - 9 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 282 IS - 26 N2 - Previous studies have shown that raising cytosolic calcium in myotubes induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis. This finding suggests that the increases in cytosolic calcium in skeletal muscle during exercise may mediate the exercise-induced increase in mitochondria. The initial aim of this study was to determine whether raising calcium in skeletal muscle induces the same adaptations as in myotubes. We found that treatment of rat epitrochlearis muscles with a concentration of caffeine that raises cytosolic calcium to a concentration too low to cause contraction induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis. Our second aim was to elucidate the pathway by which calcium induces these adaptations. Raising cytosolic calcium has been shown to activate calcium/calmodulin-dependent protein kinase in muscle. In the present study raising cytosolic calcium resulted in increases in phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor-2, which were blocked by the calcium/calmodulin-dependent protein kinase inhibitor KN93 and by the p38 mitogen-activated protein kinase inhibitor SB202190. The increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis were also prevented by inhibiting p38 activation. We interpret these findings as evidence that p38 mitogen-activated protein kinase is downstream of calcium/calmodulin-dependent protein kinase in a signaling pathway by which increases in cytosolic calcium lead to increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha expression and mitochondrial biogenesis in muscle. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/17488713/Calcium_induces_increases_in_peroxisome_proliferator_activated_receptor_gamma_coactivator_1alpha_and_mitochondrial_biogenesis_by_a_pathway_leading_to_p38_mitogen_activated_protein_kinase_activation_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=17488713 DB - PRIME DP - Unbound Medicine ER -