Tags

Type your tag names separated by a space and hit enter

Vascular fluorescence casting and imaging cryomicrotomy for computerized three-dimensional renal arterial reconstruction.
BJU Int. 2007 Aug; 100(2):387-91.BI

Abstract

Authors from the Netherlands studied the use of a casting technique, cryomicrotome imaging and three-dimensional computer analysis to visualise and reconstruct the arterial anatomy in a porcine kidney model. They found that this could be done satisfactorily to a resolution of 50 microm.

OBJECTIVE

To assess the combined use of a casting technique, cryomicrotomy imaging, and three-dimensional (3D) computer analysis as a method for visualizing and reconstructing the arterial vascular tree in a porcine renal model.

MATERIAL AND METHODS

The arterial branches of two porcine kidneys were filled with a fluorescent cast, after which they were cut into slices of 50 microm in an imaging cryomicrotome. From each section, digital images of the cutting plane of the sample were taken and stored in the computer, after which stacks of images were rendered in 3D.

RESULTS

A 3D computerized reconstruction of the arterial vascular tree was constructed and showed the complete arterial anatomy up to arterioles of 50 microm.

CONCLUSION

With visualization by fluorescence imaging cryomicrotomy, the anatomical and 3D reconstruction of the renal arterial blood supply in a pig kidney is possible up to a resolution of 50 microm.

Authors+Show Affiliations

Department of Urology, St Lucas Andreas Hospital and Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17498198

Citation

Lagerveld, Brunolf W., et al. "Vascular Fluorescence Casting and Imaging Cryomicrotomy for Computerized Three-dimensional Renal Arterial Reconstruction." BJU International, vol. 100, no. 2, 2007, pp. 387-91.
Lagerveld BW, ter Wee RD, de la Rosette JJ, et al. Vascular fluorescence casting and imaging cryomicrotomy for computerized three-dimensional renal arterial reconstruction. BJU Int. 2007;100(2):387-91.
Lagerveld, B. W., ter Wee, R. D., de la Rosette, J. J., Spaan, J. A., & Wijkstra, H. (2007). Vascular fluorescence casting and imaging cryomicrotomy for computerized three-dimensional renal arterial reconstruction. BJU International, 100(2), 387-91.
Lagerveld BW, et al. Vascular Fluorescence Casting and Imaging Cryomicrotomy for Computerized Three-dimensional Renal Arterial Reconstruction. BJU Int. 2007;100(2):387-91. PubMed PMID: 17498198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vascular fluorescence casting and imaging cryomicrotomy for computerized three-dimensional renal arterial reconstruction. AU - Lagerveld,Brunolf W, AU - ter Wee,Rene D, AU - de la Rosette,Jean J M C H, AU - Spaan,Jos A E, AU - Wijkstra,Hessel, Y1 - 2007/05/10/ PY - 2007/5/15/pubmed PY - 2007/9/21/medline PY - 2007/5/15/entrez SP - 387 EP - 91 JF - BJU international JO - BJU Int. VL - 100 IS - 2 N2 - UNLABELLED: Authors from the Netherlands studied the use of a casting technique, cryomicrotome imaging and three-dimensional computer analysis to visualise and reconstruct the arterial anatomy in a porcine kidney model. They found that this could be done satisfactorily to a resolution of 50 microm. OBJECTIVE: To assess the combined use of a casting technique, cryomicrotomy imaging, and three-dimensional (3D) computer analysis as a method for visualizing and reconstructing the arterial vascular tree in a porcine renal model. MATERIAL AND METHODS: The arterial branches of two porcine kidneys were filled with a fluorescent cast, after which they were cut into slices of 50 microm in an imaging cryomicrotome. From each section, digital images of the cutting plane of the sample were taken and stored in the computer, after which stacks of images were rendered in 3D. RESULTS: A 3D computerized reconstruction of the arterial vascular tree was constructed and showed the complete arterial anatomy up to arterioles of 50 microm. CONCLUSION: With visualization by fluorescence imaging cryomicrotomy, the anatomical and 3D reconstruction of the renal arterial blood supply in a pig kidney is possible up to a resolution of 50 microm. SN - 1464-4096 UR - https://www.unboundmedicine.com/medline/citation/17498198/Vascular_fluorescence_casting_and_imaging_cryomicrotomy_for_computerized_three_dimensional_renal_arterial_reconstruction_ L2 - https://doi.org/10.1111/j.1464-410X.2007.06914.x DB - PRIME DP - Unbound Medicine ER -