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Fabrication and characterization of Meldola's blue/zinc oxide hybrid electrodes for efficient detection of the reduced form of nicotinamide adenine dinucleotide at low potential.
Anal Chim Acta. 2007 May 29; 592(1):36-44.AC

Abstract

We report the synthesis and the electrochemical properties of hybrid films made of zinc oxide (ZnO) and Meldola's blue dye (MB) using cyclic voltammetry (CV). MB/ZnO hybrid films were electrochemically deposited onto glassy carbon, gold and indium tin oxide-coated glass (ITO) electrodes at room temperature (25+/-2 degrees C) from the bath solution containing 0.1 M Zn(NO3)2, 0.1 M KNO3 and 1x10(-4) MMB. The surface morphology and deposition kinetics of MB/ZnO hybrid films were studied by means of scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical quartz crystal microbalance (EQCM) techniques, respectively. SEM and AFM images of MB/ZnO hybrid films have revealed that the surfaces are well crystallized, porous and micro structured. MB molecules were immobilized and strongly fixed in a transparent inorganic matrix. MB/ZnO hybrid films modified glassy carbon electrode (MB/ZnO/GC) showed one reversible redox couple centered at formal potential (E0') -0.12 V (pH 6.9). The surface coverage (gamma) of the MB immobilized on ZnO/GC was about 9.86x10(-12) mol cm(-2) and the electron transfer rate constant (ks) was determined to be 38.9 s(-1). The MB/ZnO/GC electrode acted as a sensor and displayed an excellent specific electrocatalytic response to the oxidation of nicotinamide adenine dinucleotide (NADH). The linear response range between 50 and 300 microM NADH concentration at pH 6.9 was observed with a detection limit of 10 microM (S/N=3). The electrode was stable during the time it was used for the full study (about 1 month) without a notable decrease in current. Indeed, dopamine (DA), ascorbic acid (AA), acetaminophen (AP) and uric acid (UA) did not show any interference during the detection of NADH at this modified electrode.

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Authors+Show Affiliations

Kumar SA
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC. sakumar80@gmail.com
Chen SM
No affiliation info available

MeSH

ElectrodesMicroscopy, Atomic ForceMicroscopy, Electron, ScanningNADOxazinesOxidation-ReductionZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17499068

Citation

Kumar, S Ashok, and Shen-Ming Chen. "Fabrication and Characterization of Meldola's Blue/zinc Oxide Hybrid Electrodes for Efficient Detection of the Reduced Form of Nicotinamide Adenine Dinucleotide at Low Potential." Analytica Chimica Acta, vol. 592, no. 1, 2007, pp. 36-44.
Kumar SA, Chen SM. Fabrication and characterization of Meldola's blue/zinc oxide hybrid electrodes for efficient detection of the reduced form of nicotinamide adenine dinucleotide at low potential. Anal Chim Acta. 2007;592(1):36-44.
Kumar, S. A., & Chen, S. M. (2007). Fabrication and characterization of Meldola's blue/zinc oxide hybrid electrodes for efficient detection of the reduced form of nicotinamide adenine dinucleotide at low potential. Analytica Chimica Acta, 592(1), 36-44.
Kumar SA, Chen SM. Fabrication and Characterization of Meldola's Blue/zinc Oxide Hybrid Electrodes for Efficient Detection of the Reduced Form of Nicotinamide Adenine Dinucleotide at Low Potential. Anal Chim Acta. 2007 May 29;592(1):36-44. PubMed PMID: 17499068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication and characterization of Meldola's blue/zinc oxide hybrid electrodes for efficient detection of the reduced form of nicotinamide adenine dinucleotide at low potential. AU - Kumar,S Ashok, AU - Chen,Shen-Ming, Y1 - 2007/04/21/ PY - 2007/02/04/received PY - 2007/04/03/revised PY - 2007/04/04/accepted PY - 2007/5/15/pubmed PY - 2007/6/1/medline PY - 2007/5/15/entrez SP - 36 EP - 44 JF - Analytica chimica acta JO - Anal Chim Acta VL - 592 IS - 1 N2 - We report the synthesis and the electrochemical properties of hybrid films made of zinc oxide (ZnO) and Meldola's blue dye (MB) using cyclic voltammetry (CV). MB/ZnO hybrid films were electrochemically deposited onto glassy carbon, gold and indium tin oxide-coated glass (ITO) electrodes at room temperature (25+/-2 degrees C) from the bath solution containing 0.1 M Zn(NO3)2, 0.1 M KNO3 and 1x10(-4) MMB. The surface morphology and deposition kinetics of MB/ZnO hybrid films were studied by means of scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical quartz crystal microbalance (EQCM) techniques, respectively. SEM and AFM images of MB/ZnO hybrid films have revealed that the surfaces are well crystallized, porous and micro structured. MB molecules were immobilized and strongly fixed in a transparent inorganic matrix. MB/ZnO hybrid films modified glassy carbon electrode (MB/ZnO/GC) showed one reversible redox couple centered at formal potential (E0') -0.12 V (pH 6.9). The surface coverage (gamma) of the MB immobilized on ZnO/GC was about 9.86x10(-12) mol cm(-2) and the electron transfer rate constant (ks) was determined to be 38.9 s(-1). The MB/ZnO/GC electrode acted as a sensor and displayed an excellent specific electrocatalytic response to the oxidation of nicotinamide adenine dinucleotide (NADH). The linear response range between 50 and 300 microM NADH concentration at pH 6.9 was observed with a detection limit of 10 microM (S/N=3). The electrode was stable during the time it was used for the full study (about 1 month) without a notable decrease in current. Indeed, dopamine (DA), ascorbic acid (AA), acetaminophen (AP) and uric acid (UA) did not show any interference during the detection of NADH at this modified electrode. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17499068/Fabrication_and_characterization_of_Meldola's_blue/zinc_oxide_hybrid_electrodes_for_efficient_detection_of_the_reduced_form_of_nicotinamide_adenine_dinucleotide_at_low_potential_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)00742-8 DB - PRIME DP - Unbound Medicine ER -