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Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection.
Phytochem Anal. 2007 May-Jun; 18(3):204-8.PA

Abstract

An analytical procedure was developed for the simultaneous determination of total hypericin (protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin in Hypericum perforatum (St. John's wort) extracts and its preparations. The determination of total hypericin and hyperforin in one step was achieved by exposing the samples to artificial daylight in amber glass vials. This procedure allows both the photoconversion of the protoforms into the appropriate hypericins and the protection of the photosensitive hyperforin. For quantification, an HPLC method with electrochemical detection was applied. As an example of the application of the principle, two preparations containing St. John's wort were assayed.

Authors+Show Affiliations

Institut für Pharmazeutische Wissenschaften, Karl-Franzens-Universität Graz, Schubertstrasse 1, A-8010 Graz, Austria.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17500362

Citation

Rückert, Ulla, et al. "Simultaneous Determination of Total Hypericin and Hyperforin in St. John's Wort Extracts By HPLC With Electrochemical Detection." Phytochemical Analysis : PCA, vol. 18, no. 3, 2007, pp. 204-8.
Rückert U, Likussar W, Michelitsch A. Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection. Phytochem Anal. 2007;18(3):204-8.
Rückert, U., Likussar, W., & Michelitsch, A. (2007). Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection. Phytochemical Analysis : PCA, 18(3), 204-8.
Rückert U, Likussar W, Michelitsch A. Simultaneous Determination of Total Hypericin and Hyperforin in St. John's Wort Extracts By HPLC With Electrochemical Detection. Phytochem Anal. 2007;18(3):204-8. PubMed PMID: 17500362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection. AU - Rückert,Ulla, AU - Likussar,Werner, AU - Michelitsch,Astrid, PY - 2007/5/16/pubmed PY - 2007/6/6/medline PY - 2007/5/16/entrez SP - 204 EP - 8 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 18 IS - 3 N2 - An analytical procedure was developed for the simultaneous determination of total hypericin (protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin in Hypericum perforatum (St. John's wort) extracts and its preparations. The determination of total hypericin and hyperforin in one step was achieved by exposing the samples to artificial daylight in amber glass vials. This procedure allows both the photoconversion of the protoforms into the appropriate hypericins and the protection of the photosensitive hyperforin. For quantification, an HPLC method with electrochemical detection was applied. As an example of the application of the principle, two preparations containing St. John's wort were assayed. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/17500362/Simultaneous_determination_of_total_hypericin_and_hyperforin_in_St__John's_wort_extracts_by_HPLC_with_electrochemical_detection_ L2 - https://doi.org/10.1002/pca.969 DB - PRIME DP - Unbound Medicine ER -