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Human fetal testis: source of estrogen and target of estrogen action.
Hum Reprod. 2007 Jul; 22(7):1885-92.HR

Abstract

BACKGROUND

Estrogens are involved in masculine fertility and spermatogenesis. However, little is known about estrogen involvement in human testicular organogenesis. Therefore the aim of this study was to investigate the cellular sources and targets of estrogens and their variations in the human testis during fetal development. Expression profiles of aromatase (CYP19) and estrogen receptors (ER) alpha and beta were analysed in human fetal testes at various gestational stages by immunohistochemistry and quantitative RT-PCR.

METHODS

Fifty-four archival paraffin-embedded and four frozen fetal testes were studied by immunohistochemistry and real-time PCR. Tissue quality was confirmed by histology and expression of specific functional markers: androgenic enzymes for Leydig cells, anti-Müllerian hormone for Sertoli cells and Steel factor receptor for germ cells.

RESULTS

We demonstrate that the human fetal testes express aromatase and ERbeta simultaneously in Sertoli, Leydig and germ cells but are devoid of ERalpha. Quantification of positive cells indicates a window of protein expression, especially between 13 and 22-24 weeks. Quantitative RT-PCR confirmed that the human fetal testis expresses CYP19 and ERbeta but not ERalpha mRNA.

CONCLUSIONS

Our findings suggest that locally produced estrogens influence human testicular development through autocrine and paracrine mechanisms, most notably during the period of maximal testicular susceptibility to endocrine disruptors.

Authors+Show Affiliations

University Paris-Sud, UMR-S 693 Le kremlin Bicêtre Cedex F-94276, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17504822

Citation

Boukari, Kahina, et al. "Human Fetal Testis: Source of Estrogen and Target of Estrogen Action." Human Reproduction (Oxford, England), vol. 22, no. 7, 2007, pp. 1885-92.
Boukari K, Ciampi ML, Guiochon-Mantel A, et al. Human fetal testis: source of estrogen and target of estrogen action. Hum Reprod. 2007;22(7):1885-92.
Boukari, K., Ciampi, M. L., Guiochon-Mantel, A., Young, J., Lombès, M., & Meduri, G. (2007). Human fetal testis: source of estrogen and target of estrogen action. Human Reproduction (Oxford, England), 22(7), 1885-92.
Boukari K, et al. Human Fetal Testis: Source of Estrogen and Target of Estrogen Action. Hum Reprod. 2007;22(7):1885-92. PubMed PMID: 17504822.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human fetal testis: source of estrogen and target of estrogen action. AU - Boukari,Kahina, AU - Ciampi,Maria Luisa, AU - Guiochon-Mantel,Anne, AU - Young,Jacques, AU - Lombès,Marc, AU - Meduri,Geri, Y1 - 2007/05/15/ PY - 2007/5/17/pubmed PY - 2007/9/27/medline PY - 2007/5/17/entrez SP - 1885 EP - 92 JF - Human reproduction (Oxford, England) JO - Hum Reprod VL - 22 IS - 7 N2 - BACKGROUND: Estrogens are involved in masculine fertility and spermatogenesis. However, little is known about estrogen involvement in human testicular organogenesis. Therefore the aim of this study was to investigate the cellular sources and targets of estrogens and their variations in the human testis during fetal development. Expression profiles of aromatase (CYP19) and estrogen receptors (ER) alpha and beta were analysed in human fetal testes at various gestational stages by immunohistochemistry and quantitative RT-PCR. METHODS: Fifty-four archival paraffin-embedded and four frozen fetal testes were studied by immunohistochemistry and real-time PCR. Tissue quality was confirmed by histology and expression of specific functional markers: androgenic enzymes for Leydig cells, anti-Müllerian hormone for Sertoli cells and Steel factor receptor for germ cells. RESULTS: We demonstrate that the human fetal testes express aromatase and ERbeta simultaneously in Sertoli, Leydig and germ cells but are devoid of ERalpha. Quantification of positive cells indicates a window of protein expression, especially between 13 and 22-24 weeks. Quantitative RT-PCR confirmed that the human fetal testis expresses CYP19 and ERbeta but not ERalpha mRNA. CONCLUSIONS: Our findings suggest that locally produced estrogens influence human testicular development through autocrine and paracrine mechanisms, most notably during the period of maximal testicular susceptibility to endocrine disruptors. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/17504822/Human_fetal_testis:_source_of_estrogen_and_target_of_estrogen_action_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/dem091 DB - PRIME DP - Unbound Medicine ER -