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Effects of dietary strong acid anion challenge on regulation of acid-base balance in sheep.
J Anim Sci. 2007 Sep; 85(9):2222-9.JA

Abstract

The acid-base status of the extracellular fluid is directly affected by the concentrations of strong basic cations and strong acid anions that are absorbed into the bloodstream from the diet. The objective of this study was to develop and characterize a model for dietary acid challenge in sheep by decreasing the dietary cation-anion difference (DCAD) using NutriChlor (HCl-treated canola meal), an anionic feed supplement. Ten fully fleeced sheep (Rideau-Arcott, 54.3 +/- 6.7 kg of BW) were fed either a control supplement [200 g/d of canola meal, DCAD = 184 mEq/kg of DM, calculated as (Na+ + K+) - (Cl- + S2-)] or an anionic supplement (AS; 200 g/d of NutriChlor, DCAD = -206 mEq/kg of DM) offered twice daily at 0700 and 1100 in a randomized complete block design. The sheep were individually housed and limit-fed a basal diet of dehydrated alfalfa pellets (22% CP and 1.2 Mcal of NE(g)/kg, DM basis) at 1.1 kg of DM/d offered twice daily at 1000 and 1300. Two days before the beginning of the experiment, the sheep were fitted with vinyl catheters (0.86-mm i.d., 1.32-mm o.d.) in the left jugular vein to facilitate blood sampling. Blood and urine samples were obtained daily from 1100 to 1130 on d 1 through 9 and at 0700, 1000, 1300, 1600, and 1900 on d 10. Blood was analyzed for hematocrit, plasma pH, gases, strong ions, and total protein. Urine samples were analyzed for pH. The AS induced a nonrespiratory acid-base disturbance associated with lower (P < 0.05) plasma pH (7.47 vs. 7.39), lower (P < 0.05) urine pH (8.13 vs. 6.09), and lower (P < 0.05) strong ion difference (42.5 vs. 39.5). The AS reduced (P < 0.05) the concentration of plasma glucose, base excess, and bicarbonate and increased (P < 0.05) the concentration of K+ and Cl-. Lowering DCAD increased (P < 0.05) Ca2+ concentrations in plasma by 13%. In conclusion, this dietary model successfully induced a significant acid-base disturbance in sheep. Although the acidifying effects of negative DCAD in the diet may have short-term prophylactic effects of elevating the concentration of Ca2+ in plasma, negative DCAD may have detrimental effects on acid-base balance.

Authors+Show Affiliations

Department of Animal and Poultry Science, University of Guelph, Ontario, Canada, N1G 2W1.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17504956

Citation

Las, J E., et al. "Effects of Dietary Strong Acid Anion Challenge On Regulation of Acid-base Balance in Sheep." Journal of Animal Science, vol. 85, no. 9, 2007, pp. 2222-9.
Las JE, Odongo NE, Lindinger MI, et al. Effects of dietary strong acid anion challenge on regulation of acid-base balance in sheep. J Anim Sci. 2007;85(9):2222-9.
Las, J. E., Odongo, N. E., Lindinger, M. I., AlZahal, O., Shoveller, A. K., Matthews, J. C., & McBride, B. W. (2007). Effects of dietary strong acid anion challenge on regulation of acid-base balance in sheep. Journal of Animal Science, 85(9), 2222-9.
Las JE, et al. Effects of Dietary Strong Acid Anion Challenge On Regulation of Acid-base Balance in Sheep. J Anim Sci. 2007;85(9):2222-9. PubMed PMID: 17504956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary strong acid anion challenge on regulation of acid-base balance in sheep. AU - Las,J E, AU - Odongo,N E, AU - Lindinger,M I, AU - AlZahal,O, AU - Shoveller,A K, AU - Matthews,J C, AU - McBride,B W, Y1 - 2007/05/15/ PY - 2007/5/17/pubmed PY - 2007/9/25/medline PY - 2007/5/17/entrez SP - 2222 EP - 9 JF - Journal of animal science JO - J. Anim. Sci. VL - 85 IS - 9 N2 - The acid-base status of the extracellular fluid is directly affected by the concentrations of strong basic cations and strong acid anions that are absorbed into the bloodstream from the diet. The objective of this study was to develop and characterize a model for dietary acid challenge in sheep by decreasing the dietary cation-anion difference (DCAD) using NutriChlor (HCl-treated canola meal), an anionic feed supplement. Ten fully fleeced sheep (Rideau-Arcott, 54.3 +/- 6.7 kg of BW) were fed either a control supplement [200 g/d of canola meal, DCAD = 184 mEq/kg of DM, calculated as (Na+ + K+) - (Cl- + S2-)] or an anionic supplement (AS; 200 g/d of NutriChlor, DCAD = -206 mEq/kg of DM) offered twice daily at 0700 and 1100 in a randomized complete block design. The sheep were individually housed and limit-fed a basal diet of dehydrated alfalfa pellets (22% CP and 1.2 Mcal of NE(g)/kg, DM basis) at 1.1 kg of DM/d offered twice daily at 1000 and 1300. Two days before the beginning of the experiment, the sheep were fitted with vinyl catheters (0.86-mm i.d., 1.32-mm o.d.) in the left jugular vein to facilitate blood sampling. Blood and urine samples were obtained daily from 1100 to 1130 on d 1 through 9 and at 0700, 1000, 1300, 1600, and 1900 on d 10. Blood was analyzed for hematocrit, plasma pH, gases, strong ions, and total protein. Urine samples were analyzed for pH. The AS induced a nonrespiratory acid-base disturbance associated with lower (P < 0.05) plasma pH (7.47 vs. 7.39), lower (P < 0.05) urine pH (8.13 vs. 6.09), and lower (P < 0.05) strong ion difference (42.5 vs. 39.5). The AS reduced (P < 0.05) the concentration of plasma glucose, base excess, and bicarbonate and increased (P < 0.05) the concentration of K+ and Cl-. Lowering DCAD increased (P < 0.05) Ca2+ concentrations in plasma by 13%. In conclusion, this dietary model successfully induced a significant acid-base disturbance in sheep. Although the acidifying effects of negative DCAD in the diet may have short-term prophylactic effects of elevating the concentration of Ca2+ in plasma, negative DCAD may have detrimental effects on acid-base balance. SN - 1525-3163 UR - https://www.unboundmedicine.com/medline/citation/17504956/Effects_of_dietary_strong_acid_anion_challenge_on_regulation_of_acid_base_balance_in_sheep_ L2 - https://academic.oup.com/jas/article-lookup/doi/10.2527/jas.2007-0036 DB - PRIME DP - Unbound Medicine ER -