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Microsatellite instability markers for identifying early-onset colorectal cancers caused by germ-line mutations in DNA mismatch repair genes.
Clin Cancer Res. 2007 May 15; 13(10):2865-9.CC

Abstract

PURPOSE

Microsatellite instability (MSI) testing of colorectal cancer tumors is used as a screening tool to identify patients most likely to be mismatch repair (MMR) gene mutation carriers. We wanted to examine which microsatellite markers currently used to detect MSI best predict early-onset colorectal cancer caused by germ-line mutations in MMR genes.

EXPERIMENTAL DESIGN

Invasive primary tumors from a population-based sample of 107 cases of colorectal cancer diagnosed before age 45 years and tested for germ-line mutations in MLH1, MSH2, MSH6, and PMS2 and MMR protein expression were screened for MSI using the National Cancer Institute panel and an expanded 10-microsatellite marker panel.

RESULTS

The National Cancer Institute five-marker panel system scored 31 (29%) as (NCI)MSI-High, 13 (12%) as (NCI)MSI-Low, and 63 (59%) as (NCI)MS-Stable. The 10-marker panel classified 18 (17%) as (10)MSI-High, 17 (16%) as (10)MSI-Low, and 72 (67%) as (10)MS-Stable. Of the 26 cancers that lacked the expression of at least one MMR gene, 24 (92%) were positive for some level of MSI (using either microsatellite panel). The mononucleotide repeats Bat26, Bat40, and Myb were unstable in all (10)MSI-High cancers and all MLH1 and MSH2 mutation carriers (100% sensitive). Bat40 and Bat25 were unstable in all tumors of MSH6 mutation carriers (100% sensitive). Bat40 was unstable in all MMR gene mutation carriers (100% sensitive). By incorporating seven mononucleotide repeats markers into the 10-marker panel, we were able to distinguish the carriers of MSH6 mutations (all scored (10)MSI-Low) from the MLH1 and MSH2 mutation carriers (all scored (10)MSI-High).

CONCLUSIONS

In early-onset colorectal cancer, a microsatellite panel containing a high proportion of mononuclear repeats can distinguish between tumors caused by MLH1 and MSH2 mutations from those caused by MSH6 mutations.

Authors+Show Affiliations

Genetic Epidemiology Laboratory, Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17504984

Citation

Mead, Leeanne J., et al. "Microsatellite Instability Markers for Identifying Early-onset Colorectal Cancers Caused By Germ-line Mutations in DNA Mismatch Repair Genes." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 13, no. 10, 2007, pp. 2865-9.
Mead LJ, Jenkins MA, Young J, et al. Microsatellite instability markers for identifying early-onset colorectal cancers caused by germ-line mutations in DNA mismatch repair genes. Clin Cancer Res. 2007;13(10):2865-9.
Mead, L. J., Jenkins, M. A., Young, J., Royce, S. G., Smith, L., St John, D. J., Macrae, F., Giles, G. G., Hopper, J. L., & Southey, M. C. (2007). Microsatellite instability markers for identifying early-onset colorectal cancers caused by germ-line mutations in DNA mismatch repair genes. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 13(10), 2865-9.
Mead LJ, et al. Microsatellite Instability Markers for Identifying Early-onset Colorectal Cancers Caused By Germ-line Mutations in DNA Mismatch Repair Genes. Clin Cancer Res. 2007 May 15;13(10):2865-9. PubMed PMID: 17504984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microsatellite instability markers for identifying early-onset colorectal cancers caused by germ-line mutations in DNA mismatch repair genes. AU - Mead,Leeanne J, AU - Jenkins,Mark A, AU - Young,Joanne, AU - Royce,Simon G, AU - Smith,Letitia, AU - St John,D James B, AU - Macrae,Finlay, AU - Giles,Graham G, AU - Hopper,John L, AU - Southey,Melissa C, PY - 2007/5/17/pubmed PY - 2007/8/19/medline PY - 2007/5/17/entrez SP - 2865 EP - 9 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin. Cancer Res. VL - 13 IS - 10 N2 - PURPOSE: Microsatellite instability (MSI) testing of colorectal cancer tumors is used as a screening tool to identify patients most likely to be mismatch repair (MMR) gene mutation carriers. We wanted to examine which microsatellite markers currently used to detect MSI best predict early-onset colorectal cancer caused by germ-line mutations in MMR genes. EXPERIMENTAL DESIGN: Invasive primary tumors from a population-based sample of 107 cases of colorectal cancer diagnosed before age 45 years and tested for germ-line mutations in MLH1, MSH2, MSH6, and PMS2 and MMR protein expression were screened for MSI using the National Cancer Institute panel and an expanded 10-microsatellite marker panel. RESULTS: The National Cancer Institute five-marker panel system scored 31 (29%) as (NCI)MSI-High, 13 (12%) as (NCI)MSI-Low, and 63 (59%) as (NCI)MS-Stable. The 10-marker panel classified 18 (17%) as (10)MSI-High, 17 (16%) as (10)MSI-Low, and 72 (67%) as (10)MS-Stable. Of the 26 cancers that lacked the expression of at least one MMR gene, 24 (92%) were positive for some level of MSI (using either microsatellite panel). The mononucleotide repeats Bat26, Bat40, and Myb were unstable in all (10)MSI-High cancers and all MLH1 and MSH2 mutation carriers (100% sensitive). Bat40 and Bat25 were unstable in all tumors of MSH6 mutation carriers (100% sensitive). Bat40 was unstable in all MMR gene mutation carriers (100% sensitive). By incorporating seven mononucleotide repeats markers into the 10-marker panel, we were able to distinguish the carriers of MSH6 mutations (all scored (10)MSI-Low) from the MLH1 and MSH2 mutation carriers (all scored (10)MSI-High). CONCLUSIONS: In early-onset colorectal cancer, a microsatellite panel containing a high proportion of mononuclear repeats can distinguish between tumors caused by MLH1 and MSH2 mutations from those caused by MSH6 mutations. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/17504984/Microsatellite_instability_markers_for_identifying_early_onset_colorectal_cancers_caused_by_germ_line_mutations_in_DNA_mismatch_repair_genes_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=17504984 DB - PRIME DP - Unbound Medicine ER -