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RNA interference-mediated knock-down of transient receptor potential vanilloid 1 prevents forepaw inflammatory hyperalgesia in rat.
Eur J Neurosci. 2007 May; 25(10):2956-63.EJ

Abstract

Transient receptor potential vanilloid (TRPV)1 is a ligand-gated cation channel expressed by primary sensory neurons, including those in the dorsal root ganglia (DRG). TRPV1 plays an essential role in development of inflammatory thermal hyperalgesia after tissue injury and its expression in rat lumbar DRG is increased after hindpaw inflammation. However, the identity of factors mediating forepaw inflammatory hyperalgesia has remained elusive. Here, we examined behavioral responses to noxious thermal stimuli after forepaw inflammation in rats and found that inflammation induced by intraplantar injection of complete Freund's adjuvant significantly reduced hot-plate latency (HPL) at 50 degrees C. TRPV1 expression levels in the ipsilateral cervical DRG were also elevated after forepaw inflammation. By contrast, HPL at 56 degrees C was not shortened after forepaw inflammation and expression of TRPV2, a TRPV1 homolog, in the DRG was not increased. Paratracheal injection of short interfering RNA targeting TRPV1 blocked TRPV1 up-regulation in cervical DRG and abolished inflammation-mediated HPL reductions seen at 50 degrees C. However, thermal hyperalgesia previously established by inflammation was not reversed by short interfering RNA injection. These results indicate that: (i) enhanced TRPV1 expression in cervical DRG is closely associated with development of inflammatory thermal hyperalgesia in the forepaw after tissue injury and (ii) RNA interference targeting TRPV1 prevents inflammatory thermal hyperalgesia after forepaw injuries but does not ameliorate it when already established in a rat model of nociceptive pain representing upper limb injury in humans.

Authors+Show Affiliations

Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17509082

Citation

Kasama, Susumu, et al. "RNA Interference-mediated Knock-down of Transient Receptor Potential Vanilloid 1 Prevents Forepaw Inflammatory Hyperalgesia in Rat." The European Journal of Neuroscience, vol. 25, no. 10, 2007, pp. 2956-63.
Kasama S, Kawakubo M, Suzuki T, et al. RNA interference-mediated knock-down of transient receptor potential vanilloid 1 prevents forepaw inflammatory hyperalgesia in rat. Eur J Neurosci. 2007;25(10):2956-63.
Kasama, S., Kawakubo, M., Suzuki, T., Nishizawa, T., Ishida, A., & Nakayama, J. (2007). RNA interference-mediated knock-down of transient receptor potential vanilloid 1 prevents forepaw inflammatory hyperalgesia in rat. The European Journal of Neuroscience, 25(10), 2956-63.
Kasama S, et al. RNA Interference-mediated Knock-down of Transient Receptor Potential Vanilloid 1 Prevents Forepaw Inflammatory Hyperalgesia in Rat. Eur J Neurosci. 2007;25(10):2956-63. PubMed PMID: 17509082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA interference-mediated knock-down of transient receptor potential vanilloid 1 prevents forepaw inflammatory hyperalgesia in rat. AU - Kasama,Susumu, AU - Kawakubo,Masatomo, AU - Suzuki,Takefumi, AU - Nishizawa,Tomoko, AU - Ishida,Akiko, AU - Nakayama,Jun, Y1 - 2007/05/17/ PY - 2007/5/19/pubmed PY - 2007/8/19/medline PY - 2007/5/19/entrez SP - 2956 EP - 63 JF - The European journal of neuroscience JO - Eur J Neurosci VL - 25 IS - 10 N2 - Transient receptor potential vanilloid (TRPV)1 is a ligand-gated cation channel expressed by primary sensory neurons, including those in the dorsal root ganglia (DRG). TRPV1 plays an essential role in development of inflammatory thermal hyperalgesia after tissue injury and its expression in rat lumbar DRG is increased after hindpaw inflammation. However, the identity of factors mediating forepaw inflammatory hyperalgesia has remained elusive. Here, we examined behavioral responses to noxious thermal stimuli after forepaw inflammation in rats and found that inflammation induced by intraplantar injection of complete Freund's adjuvant significantly reduced hot-plate latency (HPL) at 50 degrees C. TRPV1 expression levels in the ipsilateral cervical DRG were also elevated after forepaw inflammation. By contrast, HPL at 56 degrees C was not shortened after forepaw inflammation and expression of TRPV2, a TRPV1 homolog, in the DRG was not increased. Paratracheal injection of short interfering RNA targeting TRPV1 blocked TRPV1 up-regulation in cervical DRG and abolished inflammation-mediated HPL reductions seen at 50 degrees C. However, thermal hyperalgesia previously established by inflammation was not reversed by short interfering RNA injection. These results indicate that: (i) enhanced TRPV1 expression in cervical DRG is closely associated with development of inflammatory thermal hyperalgesia in the forepaw after tissue injury and (ii) RNA interference targeting TRPV1 prevents inflammatory thermal hyperalgesia after forepaw injuries but does not ameliorate it when already established in a rat model of nociceptive pain representing upper limb injury in humans. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/17509082/RNA_interference_mediated_knock_down_of_transient_receptor_potential_vanilloid_1_prevents_forepaw_inflammatory_hyperalgesia_in_rat_ L2 - https://doi.org/10.1111/j.1460-9568.2007.05584.x DB - PRIME DP - Unbound Medicine ER -